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Biomedical subjects

C M Eneroth

Publications and source records attributed to C M Eneroth.

89 records · Page 5Linked to original sources
PubMedDate not supplied

The relationship between the nuclear DNA content in smears of aspirates and the prognosis of mucoepidermoid carcinoma.

Cytophometric analysis of nuclear DNA content was performed in a series of mucoepidermoid carcinomas with an extreme difference in the clinical course, which was observed during a follow-up period of 6-13 years. The prerequisite for such a study was the development of a method, which made it possible to study the nuclear DNA content in tumour cells obtained at the time of diagnosis, i.e. 6-13 years ago in the present material. A special cytochemical procedure with destaining of the original Giemsa stain, refixation and subsequent Feulgen staining of the smears of aspirates in the original cytological material was developed. The cytophotometric nuclear DNA analysis of tumour cells in smears of aspirate from mucoepidermoid carcinomas showed that higher ploidy tumours (near-triploid) had a worse prognosis when compared with near-diploid tumours. These data are supported by our previous findings that a shift from a near-diploid to a near-triploid DNA content of the tumour cell nuclei was associated with the prognostic important property of invasive growth. Thus, the cytophotometric nuclear DNA analysis of aspirated tumour cells seems to give valuable information about the malignancy degree of the individual tumour, which is not possible to obtain only from the cytomorphological features.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

Mucosal malignant melanomas of the head and neck with special reference to cases having a prolonged clinical course.

A histological re-examination and re-classification of primary mucosal tumours of the head and neck region, treated at Radiumhemmet and Karolinska Sjukhuset during the period 1927-1970, revealed that 41 tumours were malignant melanomas. All these 41 tumours were located in the nasal cavity, paranasal sinuses and oral cavity and not a single case of primary mucosal malignant melanoma was found in other locations of the head and neck region. In the present study, the long-term prognosis has been analysed. The follow-up period was at least 5 years and ranged up to 48 years. It was found that mucosal malignant melanomas had a very poor prognosis with a five year survival rate of 17% (7 of the total 41 cases) and a ten year survival rate of 7% (3 of the total of 41 cases). The unpredictability of the clinical behaviour of this tumour type is elucidated by cases with a prolonged clinical course despite a primary relatively limited surgery, repeated local recurrences and regional lymph node metastases in an early stage of the disease. Thus, there is always a never-ceasing risk of death in the tumour disease when once a malignant melanoma has occurred. For this reason a meticulous and lifelong follow-up of tumour patients is stressed, and also the value of repeated surgery of local recurrences and regional lymph node metastases.

Adult↗
PubMedDate not supplied

Radiosialometry. 1. A method for evaluation of salivary gland disorders.

The temporal course of the total 99Tcm40 acumulation in the parotoid glad after fast intravenous injection of the readionucleotide, is studied by examination with quantitative radiosialometry. The method adopted is based on the use of two collimated, symmetrically located and opposite placed NaI-detectors. The normal ranges of five evaluation parameters are obtained from a control material of 100 parotoid glands in 50 individuals without disorders of the parotoid glands. The reproducibility of these five evaluation parameters is estimated and the sensitivity and the detectability of the parameters as well as different sources of variation are discussed. Classification boundaries between normal and abnormal valves values are arbitrarily chosen form the normal ranges.

Adolescent↗
PubMedDate not supplied

Radiosialometry. II. The diagnostic accuracy of different evaluation parameters.

The temporal course of 99TcmO4 accumulation in the parotid gland after fast intravenous injection of the radionuclide was measured quantitatively by radiosialometry in 32 patients suffering from inflammatory or neoplastic diseases of the parotid glands. The diagnostic accuracy of five different evaluation parameters was compared. It was found that without reducing diagnostic accuracy, the method used could be simplified by omitting three of the five evaluation parameters. This implies a shortening of the measuring time from 18 to 10 minutes. The diagnostic accuracy of radiosialometry in 14 cases with a general engagement of the parotid parenchyma (irradiation damage, collagen sialosis and chronic recurrent parotitis) was high. Thus, all these 14 cases had abnormal radiosialometric values. Parotid tumours not causing extensive destruction of the parotid parenchyma were not detected by this method.

Collagen Diseases↗
PubMedDate not supplied

The antibacterial effect of antibiotics in treatment of maxillary sinusitis.

The antibacterial effect of antibiotic treatment in maxillary sinusitis has been assessed by studying the elimination of bacteria from the maxillary sinus during treatment with penicillin and tetracycline. As adequate antibiotic concentrations are the prerequisite for maximal antibacterial effect, the antibiotic concentrations were controlled. The antibiotic concentrations were determined in maxillary sinus secretions and/or mucosas of 113 patients with maxillary sinusitis, treated with single or repeated doses of penicillin or tetracycline, or the two antibiotics in combination. In 30 patients treated with either penicillin or tetracycline, the bacterial growth in sinus secretion was controlled before and after treatment. It was established that ordinary clinical doses of penicillin and tetracycline may, although not invariably, result in antibiotic concentrations in sinus secretion and sinus mucosa than can be regarded as adequate for treatment, i.e. for penicillin greater than or equal to 0.25 mug/ml and for tetracycline greater than or equal to 1.0 mug/ml. The necessity of achieving adequate antibiotic concentrations in sinus secretion and sinus mucosa to ensure therapeutic success was demonstrated. Thus, bacterial growth remained in the secretions of most maxillary sinuses when the concentrations of penicillin or tetracycline in the secretions were not regarded as adequate for treatment. In contrast, the bacteria were exterminated in most cases where concentrations were regarded as adequate.

Drug Therapy, Combination↗
PubMedDate not supplied

A cytochemical method of grading the malignancy of salivary gland tumours preoperatively.

Aspiration biopsy is an accurate but still a somewhat controversial method of diagnosing salivary gland tumours preoperatively. During the last few years this method has been more and more accepted dependent on its increasing reliability in diagnosing the different types of tumours, but for an adequate treatment it is also important to know the malignancy degree of the individual tumour. Since invasiveness is the most significant morphological criterion in evaluating the prognosis of a salivary gland tumour it has, however, been difficult to grade the malignancy morphologically on a cytological basis. Thus the diagnostic value of the aspiration biopsy method would increase, if beside the morphological determination of the tumour type it was possible to find a cellular criterion which reflects the grade of malignancy. In the present investigation we have studied the nuclear DNA content in non-invasive tumours and invasive tumours. In general, invasive tumours were characterized by a higher degree of abnormality with respect to the DNA content than the non-invasive tumours. In two tumour types especially studied-mucoepidermoid carcinoma and acinic cell carcinoma-the property of non-invasive growth was found to be associated with a diploid or near-diploid DNA content, whereas invasive growth was associated with a triploid or near-triploid DNA content. These data suggest that the nuclear DNA Content may be related to the morphological differentiation and particularly to the invasive properties of the salivary gland tumours. Thus, cytophotometric DNA analysis of DNA content in the smears from aspirates might be valuable in grading the malignancy of the tumours preoperatively by aspiration biopsy.

Carcinoma↗