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Biomedical subjects

C M Forsberg

Publications and source records attributed to C M Forsberg.

49 records · Page 3Linked to original sources
PubMed1980

An anticoagulant for transport of bovine blood.

Various anticoagulant were assessed in order to develop a transport media for bovine blood which would improve leukocyte stability. The effects of time, disease in the donor and different personnel and laboratory equipment were also tested. The transport media most effective for leukocyte stability was found to be Titriplex IV. This is a modification of Titriplex I by the inclusion of acetylsalicyclic acid to prevent cellular clumping and by the removal of methylene blue. Anticoagulant citrate dextroseformalin-ASA performed equally well for transport but cellular morphology was impaired. Febrile or neoplastic disease in the donor animal did not alter the efficiency of the formalin containing anticoagulants.

Animals↗
PubMed1980

The quantitation of Trypanosoma congolense in calves. Iv. In vitro culture of myeloid and erythroid marrow cells.

Myeloid and erythroid cultures were prepared from bone marrow aspirates taken from control and T. congolense infected neonatal and six-month-old calves. The aspirates obtained from the infected animals were more cellular than the controls and it was found that the removal of adherent cells prior to Ficoll separation improved the sensitivity of the cultures to colony stimulating factors and removed the background connective tissue cells and megakaryocytes from the resulting colonies. There was an apparent decrease in the number of myeloid colonies from the infected six-month-old calves, but an initial increase in myeloid colonies in the infected neonatal calves compared to their controls. There were no significant differences between infected and control calves for the numbers of erythroid colonies produced, but the degree of maturation as measured by hemoglobinization was decreased in two infected six-month-old calves at week 13 of infection.

Animals↗
PubMed1980

The quantitation of Trypanosoma congolense in calves. II. Biochemical changes.

Changes in serum electrolytes, osmolality, total proteins and protein fractions, lipid levels and organ function tests were determined in neonatal and 6-month-old calves infected with T. congolense TREU 112 and control calves. There were no consistent changes in electrolytes or osmolality for either age group of calves. There was a decrease in total serum protein in the infected 6-month-old calves, caused by decreases in the albumin and beta 2-globulin fractions. The total serum protein level remained constant in the infected neonatal calves, but there was a significant decrease in the albumin fraction which was compensated for by an increase in alpha-globulin and gamma-globulin fractions. Total serum lipids and cholesterol were decreased and triglyceride levels and erythrocyte phospholipid levels were significantly elevated in both age groups of infected calves. Tests of adrenal and hepatic function were not significantly different in infected calves compared to control calves of the same age group. Thyroid function tests showed that T3 was significantly elevated in infected 6-month-old calves, whereas T4 was significantly decreased in infected neonatal calves.

Adrenal Glands↗
PubMed1980

The quantitation of trypanosoma congolense in calves. III. A quantitative comparison of trypanosomes in jugular vein and microvasculature and tests of dispersing agents.

Hemocytometer and cytofluorometric methods were used to count trypanosomes in the blood of neonatal and six-month-old calves infected with T. congolense. The correlation between these methods was highest in the six-month-old calves because their parasitemia was greater and the accuracy of cytofluorometric counting is reduced below 1,000 trypanosomes per microliter of blood. Fine needle aspiration biopsies showed many trypanosomes marginated in cerebral cortical capillaries, but few in muscle or liver. Quantitatively, the cerebral capillaries in the infected six-month-old calves were significantly wider than their controls with fewer endothelial nuclei suggesting capillary dilatation. There were fewer trypanosomes and no significant changes in capillary width in the neonatal calves. Several drugs were tried for activity in dispersing marginated trypanosomes into axial flow. Berenil and dexamethasone had a significant effect on trypanosome counts in blood and tissue whereas, epinephrine caused a fleeting increase in parasitemia. Hetastarch, dextran and cyclophosphamide had no specific effect. Fine needle aspiration bipsies of brain, thirty minutes after Berenil injection showed that the capillaries were cleared of trypanosomes.

Animals↗
PubMed1979

The pathogenesis of Trypanosoma congolense infection in calves. III. Neutropenia and myeloid response.

Calves infected with Trypanosoma congolense TREU 112 had, at the onset of anemia, a very low total white cell count and neutropenia but with chronicity there was lymphocytosis. Infected calves had a marked reduction in granulocyte mobilization for the first 14 weeks of infection and there was reduced ability to mount an inflammatory response during the onset of anemia. Bone marrow aspiration biopsies showed marked erythroid hyperplasia in response to the anemia with a relative and likely absolute reduction in myeloid precursors and marrow granulocyte reserves.

Agranulocytosis↗
PubMed1979

The pathogenesis of Trypanosoma congolense infection in calves. IV. The kinetics of blood coagulation.

Blood coagulation studies showed there was a pronounced thrombocytopenia and hypofibrinogenemia in Holstein calves infected with Trypanosoma congolense TREU 112. There was also ineffective thrombopoiesis characterized by an increased megakaryocytic mass, reduced uptake of 35S-methionine into peripheral blood platelets and a normal platelet lifespan. There was an increased uptake of isotopic label into fibrinogen and a shortened half life indicating a consumptive error with increased peripheral use of fibrinogen. No consistent abnormalities were found in ethanol gelation, partial thromboplastin time, clot retraction and lysis or plasminogen assay. Fibrin split products were rarely detected. These findings suggest that in the chronic form of bovine trypanosomiasis there is a partially compensated consumption coagulopathy.

Animals↗
PubMed1979

The pathogenesis of Trypanosoma congolense infection in calves. V. Quantitative histological changes.

Calves infected with Trypanosoma congolense TREU 112 had generalized microvascular dilation which was most prominent in the liver and the mesentery; there also was increase in cellularity in the lung with significant alveolar thickening and accumulation of hemosiderin-bearing cells in the alveolar septa, focal accumulations of lymphocytes primarily at the corticomedullary junction of the kidneys and a well developed glomerulonephritis, likely membranoproliferative. Reticuloendothelial changes were marrow hyperplasia with an erythroid shift, moderate hemosiderosis and moderate dysthrombopoiesis, marked thymic cortical atrophy, hypersplenism and enlarged nodes with reduced cellular density, paracortical atrophy and medullary sclerosis.

Abomasum↗
PubMed1978

The pathogenesis of Trypanosoma congolense infection in calves. I. Clinical observations and gross pathological changes.

Holstein calves infected with Trypanosoma congolense TREU 112 had intermittent fever, debility and a poor hair coat. At postmortem examination the most significant findings were enlargement of the liver, kidneys and spleen and increased volume of hematopoietic marrow. A group of 13 calves infected for 18 weeks had marked enlargement of lymph nodes throughout the body and hemal nodes were prominent in the sublumbar area caudal to the kidneys. Another group infected for 7 weeks had no increase in the weight of lymph nodes. Studies with 51Cr labelled autologous red cells given shortly before postmortem examination showed that the liver was the most active organ with respect to sequestration of labelled red cells.

Animals↗
PubMed1978

The pathogenesis of Trypanosoma congolense infection in calves. II. Anemia and erythroid response.

The anemia caused by Trypanosoma conogolense TREU 112 in Holstein calves was of moderate severity and normochromic, macrocytic in the acute phase changing to normochromic, normocytic with chronicity. The anemia was hemolytic and responsive as shown by sharply decreased myeloid:erythroid ratio and increased mean corpuscular volume. 51Cr red cell labelling studies showed that red cell lifespan was halved in the acute phase and there was an increase in plasma volume. Surface organ counting of liver showed it to be the major site of red cell destruction. The anemia was partly dilutional as a result of the increased plasma volume and primarily hemolytic likely of a non-specific immune (innocent bystander) pathogenesis.

Anemia↗
PubMed1972

The effect of passage and iron on the virulence of Pseudomonas aeruginosa.

The factors concerned with the virulence of Pseudomonas aeruginosa are relatively labile and can be enhanced by passage. Virulence is further enhanced if the serum transferrin of the host is saturated with iron. These results draw attention to the possible serious consequences of accidental passage in hospitals, and focus attention on the importance of transferrin as a factor in non-specific resistance.

Animals↗