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C M Ignoffo

Publications and source records attributed to C M Ignoffo.

At least 19 recordsLinked to original sources
PubMed1992

Use of a colorimetric system to detect enzymes expressed by germinating conidia of entomopathogenic fungi.

An apiZYM system, with 19 substrates, was used to detect enzymes expressed by germinating conidia of Nomuraea rileyi (5 isolates), Nomuraea atypicola, Nomuraea anemonoides, Beauveria bassiana and Metarhizium anisopliae. Similar enzyme profiles were obtained for two of the N. rileyi isolates (Mississippi, Ecuador) regardless of whether culture medium (Sabouraud-maltose-yeast) or cuticle (from larvae of Trichoplusia ni, Heliothis zea or Heliothis virescens) were used as substrates. Centroid-clustering analysis revealed three distinct enzyme profiles.

Animals↗
PubMed1986

Restriction endonuclease cleavage patterns of commercial and serially passaged isolates of Heliothis baculovirus.

The restriction endonuclease patterns of three Baculovirus isolates (Br, Vh, El) from Heliothis zea having different passages and production histories were compared. Digestion of the ds DNA genomes of the three isolates with EcoRI, HindIII, and XhoI showed no major difference in the cleavage patterns, although submolar fragments were detected. One of the commercial isolates (Vh) was serially passaged 20 times through larvae of H. zea and a substitute host, H. virescens. EcoRI cleavage profiles of the ds DNA showed the absence of a band in the 3-megadalton region that was present in the original isolates. In addition, the cleavage pattern of the DNA from Vh passed in H. zea showed additional submolar fragments in the 15- and 6-megadalton regions. Differences also were observed in the HindIII and XhoI restriction patterns. No significant differences in virulence between the original isolate and passage isolates were detected after 20 serial passages in larvae of either H. zea or H. virescens.

Animals↗
PubMed1985

In vitro host range of five baculoviruses in lepidopteran cell lines.

The in vitro host range of five nuclear polyhedrosis viruses (NPV) was assessed in five lepidopteran cell lines from three genera. Multiple-enveloped baculoviruses of Autographa californica (ACMNPV), Trichoplusia ni (TNMNPV), and Galleria mellonella (GMMNPV) replicated in cells of T. ni, Spodoptera frugiperda, and Heliothis virescens to a titer of approximately 10(7) TCID50/ml. The multiple-enveloped baculovirus of S. frugiperda (SFMNPV) replicated only in S. frugiperda cells. The single-enveloped baculovirus of H. zea (HZSNPV) replicated in cells of H. zea and H. virescens but not in cells of H. armigera, T. ni, or S. frugiperda. Low levels of replication of ACMNPV, TNMNPV, and SFMNPV in cultures of H.zea, H. virescens, and T. ni, respectively, could not be detected by using a sensitive tritiated thymidine technique. However, two characteristically labelled peaks at densities of 1.145 and 1.245 g/ml were obtained in H. virescens cells inoculated with ACMNPV.60 min postinoculation ACMNPV particles were observed both entering and inside S. frugiperda cells but were not observed in H. zea or H. armigera cells. None of the five baculoviruses replicated in H. armigera cells.

Animals↗
PubMed1975

Entomopathogens as insecticides.

Entomopathogens, diseases of insects, are suggested as a possible new generation of safe, selective insecticides. Over a thousand pathogens have been isolated from insects. Many of these, associated with major insect pests, are potential candidates for development into microbial insecticides. Phases in the development of a microbial insecticide are discussed as well as factors (production, safety, efficacy, registration, non-technical) which may influence this development.

Bacteria↗