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Biomedical subjects

C M Jorgensen

Publications and source records attributed to C M Jorgensen.

17 recordsLinked to original sources
PubMed2001

Factors associated with sunburn in white children aged 6 months to 11 years.

OBJECTIVE: To determine the sunburn experience and factors associated with sunburn among white children aged 6 months to 11 years. METHODS: Telephone interviews were conducted with parents and primary caretakers of children, selected by random, stratified sampling, in the contiguous United States in the summer of 1998. Information was gathered on demographic characteristics of parents and children, and children's sunburn experience during the past year, protection from sun exposure, and hours per week spent outdoors. The proportion of children experiencing sunburn in the past year was calculated. Multivariate logistic regression analyses were conducted to determine factors associated with sunburn. Information for 1052 white children was available for the analyses. RESULTS: An estimated 42.6% of U.S. white children experienced one or more sunburns within the past year (95% CI 38.2-47.0). Sunburn was less common among children who ever wore hats (adjusted OR 0.59, 95% CI 0.40-0.87) and more common among children who did not always wear sunscreen (OR for using sunscreen sometimes compared with always, 2.25; 95% CI 1.31-3. 86). Sunburn was also more common among children with sun-sensitive skin and older children. CONCLUSIONS: A large proportion of U.S. white children experience sunburns. Parents and children may benefit from education about protection from sun exposure.

Age Distribution↗
PubMed2001

Observations from the CDC: CDC's Screen for Life: a National Colorectal Cancer Action Campaign.

Colorectal cancer is the second leading cause of cancer-related deaths in the United States. Despite the availability of several different screening tests for colorectal cancer, screening rates remain low. To raise awareness about colorectal cancer and encourage men and women aged 50 and older to speak with their physicians about being screened for colorectal cancer, the Centers for Disease Control and Prevention and the Health Care Financing Administration launched Screen for Life: A National Colorectal Cancer Action Campaign in 1999. The purpose of this paper is to outline the development of this multiyear, multimedia campaign, from conducting formative research to developing campaign messages and materials. Limited process evaluation results are presented.

Centers for Disease Control and Prevention, U.S.↗
PubMed2000

Colorectal cancer screening in older men and women: qualitative research findings and implications for intervention.

As part of the formative research for developing interventions to increase colorectal cancer screening in men and women aged 50 and older, 14 focus groups were conducted to identify (1) knowledge, attitudes, and beliefs about colorectal cancer and colorectal cancer screening, (2) barriers to screening, and (3) strategies for motivating and supporting behavior change. Participants had either private insurance or Medicare and reported different levels of experience with colorectal cancer screening. Overall, they were poorly informed about colorectal cancer and the possible benefits of screening, reporting little or no information from physicians or mass media, negative attitudes toward screening procedures, and fear of cancer. Despite references to the subject matter as embarrassing or private, both men and women, African Americans and whites, appeared to talk candidly and comfortably in the permissive context of the focus group. This study's findings suggest that public education campaigns, decision aids, and targeted interventions are urgently needed to put colorectal cancer screening on the public's "radar screen," to increase awareness of the prevention and early detection benefits of screening, and to encourage people 50 and older-and the health care providers who serve them-to make screening a high priority.

Aged↗
PubMed1999

Persistent transgene expression and normal differentiation of immortalized human keratinocytes in vivo.

Cells transduced ex vivo with transgenes encoded on retroviruses have constant and prolonged expression in vitro; however, in vivo expression is quickly lost. Much attention has been directed at methods to circumvent this problem. We have shown that loss of transgene expression does not occur when transduced immortalized 3T3 cells are transplanted to the in vivo setting of athymic mice. Ease of acquisition and potential for clinical application led us to assess the potential of using immortalized human keratinocytes for expression of transgenes in vivo. Human keratinocytes were immortalized with a HPV16-E6/E7 retrovirus, transduced with a lacZ retrovirus, cloned by limiting dilution, seeded onto a physiologic dermal substrate, and transplanted to athymic mice. Six weeks after transplantation, the immortalized transgene expressing keratinocytes had formed an epidermis that was indistinguishable from one formed by nonimmortalized keratinocytes; furthermore, there was no loss of expression of the lacZ gene. These observations show that methods to extend cell survival are an alternative approach to achieving stable and prolonged expression of transgenes in vivo and that HPV16-E6/ E7 immortalized keratinocytes generate an epidermis with normal morphology.

Animals↗
PubMed1998

Concerns and dilemmas of Hispanic AIDS information seekers: Spanish-speaking callers to the CDC National AIDS hotline.

Hispanic communities suffer disproportionately from the impact of human immunodeficiency virus infection (HIV). Each year, thousands of Spanish-speaking Hispanics call the Centers for Disease Control and Prevention National AIDS Hotline to ask questions about HIV. During 1995 alone, Spanish line staff answered more than 29,000 calls. This article presents a profile of callers and their concerns based on a systematic sample of these calls (N = 6,933) and qualitative data. The authors triangulated quantitative and qualitative results to provide a deeper understanding of the issues and dilemmas discussed with callers. Males and females called in equal numbers, but significant gender differences were observed in both situational and content variables. Gender roles, cultural values, and anxiety strongly affect the way that callers approach information and prevention. Findings suggest that health educators need to carefully examine whether prevention programs will reinforce or challenge traditional gender roles, sexual norms, and cultural values.

Acculturation↗
PubMed1997

Use of cloned genetically modified human fibroblasts to assess long-term survival in vivo.

Because human fibroblasts are easily brought to tissue culture conditions and can be stably transduced with retroviral vectors encoding transgenes ex vivo, genetically modified fibroblasts are frequently considered in strategies to correct disease with gene therapy. This enthusiasm has been dampened by studies showing that transgene expression by genetically modified fibroblasts diminishes with time in vivo, but not in vitro, for reasons that are unclear. We elected to study this problem using cloned human fibroblasts that had been cloned by limiting dilution and stably transduced with a retroviral vector encoding lacZ ex vivo. These were seeded onto a nonbiodegradable nylon matrix that was transplanted to nude mice. Transgene expression was followed prospectively by histologic exam. Data show that human fibroblasts can withstand the pressure of cloning by limiting dilution. In addition, they can be passaged from 10 to > 20 times, and > 1 x 10(20) of genetically modified fibroblasts can be generated as progeny of one cell. Loss of transgene expression by the cloned genetically modified fibroblasts in vivo occurs in an orderly and progressive fashion, but is not complete by 4 months. Neither the loss nor the persistence of expression appear to be random. These observations are most compatible with the thesis that a major cause of the loss of transgene expression in vivo is secondary to apoptosis of the genetically modified fibroblast. Loss of expression of transgenes in senescent genetically modified fibroblasts occurs more rapidly than in their presenescent counterparts in the age-neutral, in vivo setting of the nude mouse.

Animals↗
PubMed1997

The effects of HIV counseling and testing on risk-related practices and help-seeking behavior.

In an earlier review of the behavioral effects of HIV counseling and testing (HIV CT), Higgins and colleagues (1991) found that the evidence regarding the ability of HIV CT to influence HIV-risk related practices was largely inconclusive. This article reviews 35 domestic and international studies published since that time to reassess the scientific data regarding the ability of HIV CT to motivate changes in risk-related practices and to promote help-seeking behavior. The studies identified for this review were grouped into four categories according to subject population: (1) men who have sex with men, (2) injection and other drug users, (3) women and heterosexual couples, and (4) mixed samples recruited from sexually transmitted disease (STD) clinics and other settings. Findings from the studies reviewed were generally mixed--many provided at least some evidence supporting the ability of HIV CT to motivate risk-reducing and help-seeking behavior, but others did not. The pattern of results varied substantially across, and within, study populations and were often limited by considerable methodological weaknesses.

AIDS Serodiagnosis↗
PubMed1995

Sustained production of human transferrin by transduced fibroblasts implanted into athymic mice: a model for somatic gene therapy.

Somatic gene therapy has been proposed as a means of treating inherited diseases involving defective or absent plasma proteins, viral diseases, and cancer. Introduction of the gene of interest into fibroblasts and implantation of these genetically modified fibroblasts using a skin equivalent system may be an attractive model for gene therapy because skin fibroblasts are easily obtained and propagated in culture. This study evaluated expression of the gene for human transferrin (hTf) by genetically modified fibroblasts in vitro and in vivo. NIH 3T3 fibroblasts, which form non-metastasizing tumors in athymic mice, were transduced with a retroviral vector encoding hTf. The transduced cells were cloned by limiting dilution and hTf production by the cloned cells measured. Two clones of cells producing high levels of hTf were used to seed collagen-coated nylon matrices, which were maintained in culture for up to 53 d. The rate of synthesis of hTf by the seeded matrices was constant after 22 d in vitro. Matrices seeded with cloned, transduced cells were implanted subcutaneously into seven athymic mice, and plasma levels of hTf were assessed biweekly. In all animals, the plasma level of hTf was detectable at week 6 after implantation. Levels of hTf remained elevated in the animals until the implants were removed at week 12. At week 10, the level of hTf in the plasma correlated with tumor volume in tumors less than 2000 mm3 in size. The half-life of hTf in the mice was 39.5 h. In this model, gene expression did not decline for the 12-week observation period.

3T3 Cells↗
PubMed1994

Genetically modified skin to treat disease: potential and limitations.

Molecular definition of disease at the level of the gene and advances in recombinant DNA technology suggest that many diseases are amenable to correction by genes not bearing the defective elements that result in disease. Many questions must be answered before this therapy can be used to correct chronic diseases. These questions fall into safety and efficacy categories. Experience with transplanting cellular elements of skin or skin substitutes (defined as skin that possess the cell types and a dermal structure to develop into a functioning skin) to athymic rodents is considerable and is seen as a system where these questions can be answered. This paper reviews these questions and presents our early analysis of genetically modified cells in skin substitutes in vivo and in vitro. Experimental data demonstrate that both a matrix of woven nylon, housing a fibroblast generated collage, and dead dermis can be utilized to shuttle genetically modified human fibroblasts from the laboratory to an in vivo setting. Genetically modified fibroblasts do not migrate from the shuttle to the surrounding tissue. The survival of significant numbers, approximately 70%, of genetically modified fibroblasts for at least 6 weeks in these shuttles, supports this general approach as having clinical utility. It is also concluded that skin substitute systems can be used to generate a genetically modified skin in vitro that has the capacity to develop into functional skin in vivo. Further, as genetically modified keratinocytes differentiate there is increased production by the transgene, supporting the concept that keratinocytes have true potential as shuttles for therapeutic genes. This work demonstrates that transplantation of systems containing genetically modified cells of the skin can be used to experimentally define many aspects of gene therapy using skin before this technology is taken to the clinic. Examples include determining the effect of gene transduction and expression on structure and function of the genetically modified skin as well as on distant skin and an assessment of the translational capacity of the transgene as function of time and cell number.

3T3 Cells↗
PubMed1994

Health education: what can it look like after health care reform? 1993 SOPHE presidential address.

In the fall of 1993 the plans for the Health Security Act were unveiled: health education was referenced no fewer than 18 times. This 1993 SOPHE Presidential Address examines the role of health education under the plans for and principles of health care reform. As Bill Clinton stated, "an intensified health education system must be designed to educate and encourage the American people to change behavior that results in ill health and high costs." It is argued that health education has been demonstrated to be effective at reducing risk behaviors associated with each of the leading causes of death. Likewise health education should, can, and does play a role in each of the health goals and objectives for the year 2000. Health reform provides new opportunities to invest in prevention, public health and health education--not only in medical care settings--but in schools, at worksites, and in the community. Health education in these settings can help create supportive environments that make healthy choices the easy choices, ensuring that health reform can succeed.

Cost Savings↗
PubMed1990

Experimental models for psoriasis.

Evidence suggests that inherent in skin of psoriatic subjects are cells, architectural structures, and/or mediators, which are, at a minimum, responsible for its hyperproliferative epidermis. An objective of our laboratory has been to establish an in vitro definition of this inherent aberration. Fibroblasts are important to epidermal proliferation/differentiation. This, and an unconfirmed report that fibroblasts from psoriatic subjects might drive the abnormal epidermal proliferation in psoriasis, have caused further focus on the fibroblast. Data show that fibroblasts from patients with psoriasis, both involved and uninvolved, in the presence of human serum, either normal or psoriatic, have an increased rate of proliferation. Fibroblasts from uninvolved psoriatic sites are most responsive. To determine if fibroblasts from psoriatics could induce the psoriasiform phenotype on normal keratinocytes, an interactive skin equivalent system has been developed. With this system, fibroblasts from uninvolved and involved sites cause normal keratinocytes to have an enhanced outgrowth. Uninvolved fibroblasts cause the greatest changes. The nature of the skin equivalent system calls for this to occur via message over distance. We conclude that fibroblasts from psoriatic subjects can induce a psoriasiform phenotype via a soluble message.

Animals↗
PubMed1989

Approach for and assessment of interactive communication via cytokines of cellular components of skin.

Structure and function of skin are dependent upon interaction of the cells and matrix components that are unique to skin which, in turn, are dependent upon an interactive message system of cytokines acting over distance. Our laboratory has utilized a system which is sufficiently complex to permit a component of the skin to mature into a functional unit that can be indirectly influenced by cell types inherent to the skin, message over distance. The system, human epidermal cells, maturing into an epidermis on an overlying Millicell-HA membrane in the presence of normal fibroblasts or peripheral blood mononuclear cells in a lower chamber has been used to assess the role of interactive cytokines in skin. Data demonstrate: (a) normal fibroblasts enhance the outgrowth and induce a more organized phenotype in squamous cell carcinoma cells; (b) keratinocytes enhance fibroblast proliferation while squamous cell carcinoma cells suppress fibroblast proliferation, and (c) both activated and nonactivated mononuclear cells affect the outgrowth and organization of the squamous cell carcinoma cell line. It is concluded that cells of the skin, by their mediators, do affect the growth of one another in a highly interactive way.

Biological Factors↗
PubMedDate not supplied

Protection from sun exposure in US white children ages 6 months to 11 years.

OBJECTIVES: To estimate the prevalence of protection from sun exposure among US white children ages 6 months to 11 years. METHODS: During the summer of 1998, using telephone directory lists supplemented by random-digit dialing, the authors surveyed parents living in the contiguous United States. They calculated weighted prevalence estimates for protection methods and conducted logistic regression analyses to determine parent and child characteristics predictive of protection behaviors. RESULTS: Parents of 1,055 white children were interviewed. Children spent a median of 20 hours per week outdoors during the summer, of which 10 hours were at school. Sunscreen (61.8%, 95% confidence interval [CI] 57%, 66%) and shade (26.5%, 95% CI 22%, 31%) were the most frequently reported protection methods. Parents reported higher rates of protection for younger children and children who sunburn easily. CONCLUSIONS: Parents report that a large proportion of white children is protected from sun exposure by one or more methods. Health care providers and educators might encourage the use of all methods of protection, not just sunscreen use, and educate older children to protect themselves from the sun.

Adult↗
PubMedDate not supplied

Health promotion programs in small worksites: results of a national survey.

PURPOSE: This study documents the prevalence of workplace health promotion activities at small worksites with 15 to 99 employees. DESIGN: A random sample of U.S. worksites stratified by size and industry (n = 3628) was drawn using American Business Lists. MEASURES: Each worksite was surveyed using a computer-assisted telephone interview system to document activities related to health promotion and related programs, worksite policies regarding health and safety, health insurance, and philanthropic activities. SUBJECTS: Participation varied by industry and size, with an overall response rate for eligible worksites of 78% for a total sample of 2680 worksites. DATA ANALYSIS: Data were analyzed using SUDAAN statistical software. RESULTS: Approximately 25% of worksites with 15 to 99 employees offered health promotion programs to their employees, compared with 44% of worksites with 100+ employees. As with the larger worksites, the most common programs for worksites with 15 to 99 employees were those related to occupational safety and health, back injury prevention, and CPR. The majority of worksites in both size categories had alcohol, illegal drug, smoking, and occupant protection policies. The majority of both small and large worksites also offered group health insurance to their employees (92% and 98%, respectively), with many of the worksites also extending benefits to family members and dependents (approximately 80% for both business sizes). CONCLUSIONS: The results indicated that small worksites are providing programs to their employees, with a primary focus on job-related hazards. Small worksites also have formal policies regarding alcohol, drug use, smoking, and seatbelt use and offer health insurance to their employees at a rate only slightly lower than that of large worksites.

Female↗
PubMedDate not supplied

Monitoring the exposure of "America Responds to AIDS" PSA campaign.

The "America Responds to AIDS" campaign is the focal point of an integrated mass communications system for AIDS education and information dissemination developed by the National AIDS Information and Education Program of the Centers for Disease Control. Television and radio public service announcements are an integral part of the campaign. One measure of their success is the extent to which they are aired on both national and local levels. Since 1987, the total dollar value for air time donated to the "America Responds to AIDS" campaign is more than $65 million, representing 47 percent of all donations of air time for AIDS public service announcements. These results suggest that the campaign has been successful in reaching a large proportion of the public.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

The menopausal transition: guidelines for researchers.

We believe that the menopausal transition can be eased for women if they have information about normal changes they can anticipate. Until now, research on the subject of menopause has been fragmented and flawed, in large part because an "oppositional approach" to the study of menopause has pitted biology against culture and has slowed progress toward a more fruitful interactive approach. Failure to agree on basic definitions has contributed further to fragmentation. Future research efforts must employ healthy, non-clinical samples and prospective designs that will avoid recall problems. Women must be at the center of this research, as respondents whose experiences and interpretations of these experiences become the focus of study. It is the normalcy of menopause that remains undocumented (Voda & George, 1986). When research has identified a range of experiences common to the menopausal transition for healthy women, it will be possible for aging women and their health care providers to make accurate assessments of their individual patterns of change. It is anticipated that studies will document a wider range of changes as normal than is currently accepted, perhaps reducing the number of interventions occurring when pathology is identified incorrectly. Studies with a bi-directional focus will make possible an understanding of the mutual influences of biology and culture on premenopausal women's changing experiences. Knowledge of which symptoms are inevitable conditions of hormonal shifts and which are not will remove the burden of ignorance from women and empower them to better cope with this life stage.

Female↗