PubMed Health⌕ Search

Biomedical subjects

C M KUNIN

Publications and source records attributed to C M KUNIN.

At least 19 recordsLinked to original sources
PubMed1963

SEPARATION, CHARACTERIZATION, AND BIOLOGICAL SIGNIFICANCE OF A COMMON ANTIGEN IN ENTEROBACTERIACEAE.

An antigen common to Enterobacteriaceae and closely associated with endotoxin fractions has been separated by chromatography on DEAE cellulose employing elution with a NaCl gradient. The purified common antigen fails to coat erythrocytes, is poorly, if at all antigenic, it is non-dialyzable and excluded from sephadex G-100 gel. It is composed of polysaccharide and polypeptide. The most important property of this antigen thus far determined appears to be its interference with the specificity of the hemagglutination test commonly employed to measure antibody to O antigen of Enterobacteriaceae. It may also have taxonomic significance in classification of this family of bacteria.

Antibodies↗
PubMed1963

SEROLOGICAL STUDIES OF O ANTIGENS OF ESCHERICHIA COLI BY MEANS OF THE HEMAGGLUTINATION TEST.

Kunin, Calvin M. (University of Virginia, Charlottesville) and Mary V. Beard. Serological studies of O antigens of Escherichia coli by means of the hemagglutination test. J. Bacteriol. 85:541-548. 1963.-The serological interrelationships among most of the known O antigens of Escherichia coli were studied by the hemagglutination test. Good agreement with the bacterial agglutination test was found with the major antigens, but cross reactions among groups were much more limited with the hemagglutination test. Heterogenetic cross reactions with many Enterobacteriaceae species were observed with rabbit antisera to E. coli O14, O56, O124, and O144. This effect tended to elevate antibody titers observed in human serum against almost all O antigen groups, but could not by itself account for the wide diversity of antibodies to them. E. coli antibodies were localized in human gamma-globulin, but were present in the beta-globulin fractions of a number of domestic animals. Rabbits differed from other animals studied in that antibodies to E. coli were rarely found in their sera; this was attributed in part to the low density of E. coli populations in this animal. The hemagglutination test may be used in identification of unknown O antigens, but does not have special merit over the bacterial agglutination test; it does not further characterize rough or untypable strains other than by demonstrating that they also possess heterogenetic antigens.

Agglutination Tests↗
PubMed1961

The tetracyclines.

Explore the source record for details and available documents.

Anti-Bacterial Agents↗