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Biomedical subjects

C M McCarthy

Publications and source records attributed to C M McCarthy.

At least 19 recordsLinked to original sources
PubMed1993

Low temperature protocol for efficient transformation of Mycobacterium smegmatis spheroplasts.

Spheroplasts of Mycobacterium smegmatis LM15, strain 607, were prepared by a combined treatment with glycine and lysozyme. The spheroplasts were tested for ability to take up and express purified mycobacteriophage DNA. Exposure of 1.0 x 10(8) to 1.0 x 10(9) spheroplasts to saturating DNA (1 microgram) for 15 min at 5 degrees C resulted in a transfection efficiency of approximately 0.009% . The transfer of the beta-lactamase marker with DNA purified from strain LM15 to spheroplasts of a beta-lactamase-negative mutant, strain LM144, was achieved. The DNA-treated cultures, after reversion to the bacillary form, contained 20-fold more penicillin-resistant cells than the nontreated control culture. Approximately 80% of the penicillin-resistant colonies from the DNA-treated cells were positive for beta-lactamase Cell-free extracts of penicillin-resistant transformants contained beta-lactamase activity that ranged from 0.046 to 0.134 micromol of benzylpenicillin hydrolyzed/min per mg protein. This low temperature procedure is recommended for high efficiency transformation of M. smegmatis.

Mycobacteriophages↗
PubMed1993

Incorporation of 5-fluorouracil into RNA by Mycobacterium avium complex strain LM1.

The incorporation of 5-fluorouracil (FUra) into RNA of Mycobacterium avium complex strain LM1 was evaluated. Cells were labeled with either [14C]FUra or [3H]uracil and the ribonucleosides were analyzed by high-performance liquid chromatography. The identification of the ribonucleosides was facilitated by the use of an isocratic system that provided unambiguous identification of the RNA pyrimidine components. Uracil was incorporated into RNA as uridine, but an equal amount was converted to cytidine. [14C]FUra was incorporated directly into RNA as 5-fluorouridine and there was no evidence of its conversion to other pyrimidines. The ratio of 5-fluorouridine:uridine was 2.8-fold greater for cells grown in 100 micrograms FUra/mL than for cells grown in 20 micrograms FUra/mL. Analysis of the RNA nucleotides was performed and deoxyribonucleotides were present; DNA contamination was estimated to range from about 2 to 8% of the RNA preparations.

Biotransformation↗
PubMed1991

The AHA's priorities for 1991.

The next decade presents formidable challenges for hospitals and for American health care in general. Whatever emerges from the current health care reform debates, a fundamental shift is taking place toward outpatient and non-hospital site care. Carol M. McCarthy, J.D., Ph.D, AHA president, outlines the association's plans for the coming year.

American Hospital Association↗
PubMed1991

Multilocus enzyme electrophoresis analysis of the Mycobacterium avium complex and other mycobacteria.

Multilocus enzyme electrophoresis analysis was used to evaluate the Mycobacterium avium complex (MAC), M. paratuberculosis, and nine other mycobacterial species. The average number of alleles per locus was 2.8 for the 35 MAC and 2 M. paratuberculosis strains which represented 24 electrophoretic types (ETs) and two distinct groups. The M. avium group was resolved into 17 ETs and contained the M. paratuberculosis ET. The M. intracellulare group consisted of six ETs. There was complete agreement between Gen-Probe identification and group placement by multilocus enzyme electrophoresis. The mean genetic diversity per locus for the 24 MAC ETs was 0.38. This procedure subdivided some serovars and, if implemented, should prove to be a powerful epidemiologic tool for the MAC. Eleven additional ETs were formed after the data for the other mycobacterial species were pooled with those for the MAC.

Alleles↗
PubMed1990

The AHA's priorities for 1990.

The American Hospital Association will confront a number of key issues in the coming year, writes Carol McCarthy, AHA president. The association expects to face unprecedented challenges in its efforts to promote an environment conducive to the continuation of high-quality health care services. In addition, a number of forces may combine to create a sense of urgency--inside and outside of government--for major reforms in our current system.

American Hospital Association↗
PubMed1987

Sweet's syndrome, acute leukemia, and t(3;5).

We report herein the case of a 36-year-old woman who was diagnosed as having Sweet's syndrome 13 months prior to developing acute myeloid leukemia (FAB type M2). Her bone marrow karyotype was 46,XX,t(3;5)(q21;q31). Translocation t(3;5) has been reported in seven other cases of acute nonlymphocytic leukemia. None of these cases have been associated with Sweet's syndrome.

Adult↗