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C M Murphy

Publications and source records attributed to C M Murphy.

59 records · Page 4Linked to original sources
PubMedDate not supplied

A strategy for characterization of polyethylene glycol-derivatized proteins. A mass spectrometric analysis of the attachment sites in polyethylene glycol-derivatized superoxide dismutase.

Base treatment of polyethylene glycol-derivatized superoxide dismutase in which the polyethylene glycol is linked to the protein via a succinyl bridge, removes the polyethylene glycol leaving a succinyl marker. Exhaustive succinylation with d4-succinic anhydride completes the derivatization in order to minimize fractionation in proteolysis, chromatography, and desorption in the mass spectrometer. Production of peptides from the derivatized protein for high resolution and high-resolution tandem MS allows identification of the site that had been derivatized by polyethylene glycol and the determination of the amount of polyethylene glycol originally at each site. The mass spectrometric strategy outlined herein can be applied to other proteins derivatized for therapeutic administration.

Amino Acid Sequence↗
PubMedDate not supplied

Tobacco erases 30 years of progress: preliminary analysis of the effect of tobacco smoking on Alaska Native birth weight.

OBJECTIVE: Investigate the relationship between tobacco and/or alcohol use and Alaska Native birth weight. METHODS: Data on weight, tobacco smoking and alcohol use among Alaska Natives were abstracted from 1989-91 Indian Health Service natality records based on birth certificates. RESULTS: Birth certificate data were available for 9,175 live births. Single live births were analyzed for 8,994 Alaska Natives. In women with no tobacco smoking the mean birth weight of their infants was 3,571 g; 1-5 cigarettes/day 3,429 g; 6-10 cigarettes/day 3,332 g (p < .05); and > 10 cigarettes/day 3,260 g (p < .05). Infants of Alaska Natives who reported no alcohol and no tobacco use had a mean birth weight of 3,579 g; alcohol use but no tobacco use 3,452 g; no alcohol but tobacco use 3,388 g; and both alcohol and tobacco use 3,281 g. (p < .001). CONCLUSIONS: The mean birth weight of infants born to Alaska Native women with the highest use of tobacco were reduced by over 300 g compared to non-smoking Alaska Native women. Mean infant birth weight of tobacco smoking Yup'ik women in 1989-91 were reduced by over 400 g, comparable to weights reported in the 1960s.

Alaska↗