Biomedical subjects

C M Nolte

Publications and source records attributed to C M Nolte.

3 recordsLinked to original sources

PubMed2002

Clitellocephalus americanus n. gen., n. sp. (Apicomplexa: Eugregarinida: Gregarinidae) from Cratacanthus dubius (Coleoptera: Carabidae: Harpalinae) in the Nebraska sandhills and Clitellocephalus ophoni n. comb. (Apicomplexa: Eugregarinida: Gregarinidae) from Ophonus pubescens (Coleoptera: Carabidae: Harpalinae) in Sète, France.

Clitellocephalus americanus n. gen., n. sp. (Apicomplexa: Eugregarinida: Gregarinidae) is described from Cratacanthus dubius (Coleoptera: Carabidae) collected from Keith County in the Sandhills of western Nebraska. Clitellocephalus ophoni n. comb. is redescribed using original type material from Ophonus pubescens (Coleoptera: Carabidae) collected in Sète, France. Clitellocephalus n. gen. is distinguished by a deltoid epimerite with an internal anterior obconoid structure and a basal toroidal vacuole, which is retained in gamonts. Protomerites are broadly elliptical to cylindrical; deutomerites are narrowly obovate. Association is precocious, caudofrontal, and biassociative, with the satellite protomerite engulfing the posterior end of the primite deutomerite to form an interlock. Gametocysts are spherical. Sporoducts are present but reduced and irregular in number. Oocysts are dolioform, dehiscing in chains. The species described herein are differentiated by their overall size and relative proportion of cellular structures.

Animals↗

PubMed1998

Magnetic field conditioned taste aversion in rats.

Conditioned taste aversion is a common classic conditioning procedure used to identify noxious stimuli. When a rat is given a taste solution, the conditioned stimulus (CS), followed by an unpleasant experience, the unconditioned stimulus (US), the rat will avoid consumption of the CS in future presentations. These experiments use the taste aversion procedure to examine the effect of exposure to a high magnetic field. A solution consisting of 3.0 g glucose and 1.25 g saccharin per 1 L of solution (G+S) was used as the CS and a 9.4-T magnet served as the US. In Experiment 1, all rats received a 10 min presentation of the G+S solution followed by either a 30 min exposure to the magnetic field (Magnet, n = 8), a 30-min exposure in a container with similar conditions but lacking the magnetic field (Sham, n = 8), or no exposure (Control, n = 8). The Magnet Group showed a taste aversion on the first day of preference testing (p < 0.05). Experiment 2 employed the same US-CS protocol for 3 consecutive days of conditioning. The Magnet Group demonstrated a taste aversion for the postexposure Days 1-8 (p < 0.01). There was no difference between the Sham and Control Groups in either experiment. The results of this study clearly demonstrate that the rats associated the G+S solution with the experience of being exposed to the high magnetic field and avoided the solution in subsequent presentations.

Animals↗

PubMed1991

Epidermis generated in vitro: practical considerations and applications.

The technology for culture of epidermis is one of the most advanced to date for generation of a tissue in vitro. Cultured epidermis is already used for a number of applications ranging from use as a permanent skin replacement to use as an organotypic culture model for toxicity testing and basic research. While simple epidermal sheets have been grafted successfully, more advanced models for skin replacement consisting of both dermal and epidermal components are in development and being tested in a number of laboratories. One of the most advanced in vitro models is the living skin equivalent, an organotypic model consisting of a collagen lattice contracted and nourished by dermal fibroblasts overlaid with a fully formed epidermis.

Culture Techniques↗