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Biomedical subjects

C M Shih

Publications and source records attributed to C M Shih.

43 records · Page 3Linked to original sources
PubMed1988

Exsheathment of microfilariae of Brugia pahangi in the susceptible and refractory strains of Aedes aegypti.

Exsheathment of microfilariae of Brugia pahangi was studied in susceptible (Liverpool) and refractory (Bora-Bora) strains of Aedes aegypti. It was found that the microfilariae tend to carry their sheaths into the haemocoel of both strains of Ae. aegypti within two hours after the engorgement of mosquitoes from a rat parasitized by filariae. The percentage of sheathed microfilariae in the haemocoel then progressively decreased to 0% at eight hours and to 1% at 24 hours post-ingestion in the Bora-Bora and Liverpool strains, respectively. Those microfilariae that remained in the midgut more than two hours after ingestion were most likely to cast off their sheaths there. The percentage of microfilariae exsheathed in the midgut progressively increased to about 91 and 78% at 24 hours post-ingestion in the Bora-Bora and Liverpool strains, respectively. These results suggested that the exsheathment of microfilariae occurs both in the haemocoel and in the midgut of two strains of Ae. aegypti.

Aedes↗
PubMed1988

Biomechanical analysis of posterior instrumentation systems after decompressive laminectomy. An unstable calf-spine model.

Mechanical non-destructive cyclical testing in rotation, axial compression, and flexion were performed on twelve fresh spinal segments from calves. Each segment contained five motion segments. Each spine was destabilized with bilateral laminectomy and facetectomy of the fourth and fifth lumbar vertebrae, resection of the pars interarticularis of the fourth lumbar vertebra, and resection of the disc between the fourth and fifth lumbar vertebrae. Sequential stabilization of each spine was used to compare the stiffness of: (1) Harrington distraction instrumentation of five levels, (2) Luque rectangular instrumentation of five levels, (3) modified Steffee transpedicular notched-rod instrumentation of three and five levels, and (4) Cotrel-Dubousset transpedicular instrumentation of three and five levels with and without transverse approximating rods. This in vitro study of a calf-spine model led to three reproducible conclusions: (1) after laminectomy and discectomy, the instrumented spine was more unstable in rotation and flexion than when it was subjected to axial compressive loads; (2) the most rigid implant was the Cotrel-Dubousset transpedicular instrumentation of five vertebral levels (p less than 0.05); and (3) with the Steffee or the Cotrel-Dubousset transpedicular instrumentation of three vertebral levels, it was possible to restore torsional, compressive, and flexural rigidity to the destabilized spine of the calf. Furthermore, transpedicular fixation of only three vertebral levels provided more in vitro stability than either traditional Harrington or Luque rectangular instrumentation, which require fixation of five vertebral levels to stabilize a spine after laminectomy.

Animals↗
PubMed1988

Biomechanical analysis of anterior and posterior instrumentation systems after corpectomy. A calf-spine model.

UNLABELLED: To simulate the spinal instability that is found clinically after anterior corpectomy for the treatment of a fracture or a neoplasm, twelve fresh calf-spine segments, each containing five motion segments, were destabilized using a complete anterior corpectomy at the third lumbar level and anterior discectomies at the second and third and the third and fourth lumbar levels. Mechanical non-destructive cyclical testing in axial compression, rotation, and flexion was performed on each spinal segment after stabilization was accomplished. The three anterior-stabilization constructs that were compared were: (1) iliac strut grafting, (2) polymethylmethacrylate and anterior Harrington-rod instrumentation (the technique of Siegal and Siegal), and (3) the Kaneda anterior device. After anterior iliac-crest strut grafting, four types of posterior instrumentation were also tested sequentially: (1) Harrington distraction rods, (2) Luque rectangular instrumentation, (3) Cotrel-Dubousset transpedicular instrumentation, and (4) Steffee transpedicular screws and plates. Rotation, torque, axial displacement, and axial loads were measured during loading across the whole spinal segment between the grip points. Using an anterior extensometer, intervertebral displacement at the second, third, and fourth lumbar levels, and thus across the corpectomy defect at the third lumbar level, was recorded "on line" during testing in flexion and axial load. By recording the intervertebral displacement, the efficacy of each spinal construct in minimizing motion across the corpectomy defect could be quantified. The value for one-way analysis of variance for axial intervertebral displacement across the site of the third lumbar corpectomy was F = 10.5, p less than 0.001. The value for one-way analysis of variance for flexural intervertebral displacement across the corpectomy defect was F = 21.1, p less than 0.001. Homogeneous subsets of rigidity for torsional stiffness revealed that the least rigid constructs were iliac grafting alone, Harrington-rod instrumentation, and Luque rectangular instrumentation. The most rigid constructs were the anterior Kaneda device, transpedicular Cotrel-Dubousset instrumentation, and Steffee screws and plates. CLINICAL RELEVANCE: After corpectomy, spinal reconstructive surgery can restore axial, torsional, and flexural rigidity to normal levels. These experimental conclusions applied to the acute restoration of stability, rather than to rigidity after long-term cyclical loading. Using the most rigid anterior system, the Kaneda device, the fixation extended only one vertebral level cephalad and one level caudad to the corpectomy defect.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1987

Exsheathment of microfilariae of Brugia pahangi in Anopheles quadrimaculatus and Culex quinquefasciatus.

In order to determine whether the exsheathment patterns described in our previous study occurred in other microfilaria-mosquito systems, exsheathment of microfilariae of Brugia pahangi was studied in two species of mosquitoes. The results of the quantitative observation revealed that the microfilariae of Brugia pahangi tend to carry their sheaths into the haemocoel of Anopheles quadrimaculatus and Culex quinquefasciatus within 4 hr after infected blood meals. The percentage of the sheathed microfilariae in the haemocoel progressively decreased to 0% at 24 hr post-ingestion. Microfilariae remaining in the midgut of both species of mosquitoes were recorded most frequently casting off their sheaths in the midgut 2 hr post-ingestion. The percentage of microfilariae exsheathed in the midgut progressively increased to about 100% and 40% 24 hr post-ingestion in Anopheles quadrimaculatus and Culex quinquefasciatus respectively. These results confirm that exsheathment of microfilariae of Brugia pahangi occurs both in the haemocoel and in the midgut of two species of mosquitoes.

Animals↗
PubMedDate not supplied

Hepatitis B viral polymerase fusion proteins are biologically active and can interact with the hepatitis C virus core protein in vivo.

Hepadnaviruses and retroviruses are evolutionarily related families because they both require a process of reverse transcription for genome replication. However, hepadnaviruses produce polymerase (pol) and core proteins separately, while retroviruses synthesize a gag-pol fusion protein that is subsequently cleaved by a virally encoded protease to release a functional polymerase. To test whether an additional sequence at the N-terminus of pol in hepatitis B virus (HBV) interferes with its function, we created two plasmids expressing core-pol fusion proteins, core144-pol and core31-pol. Secreted particles obtained from HuH-7 cells, which were cotransfected with a core-pol fusion protein-expressing plasmid and a core-expressing plasmid, showed a positive signal of HBV DNA by the endogenous polymerase assay, indicating that the core-pol fusion proteins retain DNA priming, polymerization and RNase H activities. The fusion protein was detected in the cytoplasm of transfected cells and in secreted virions by immunoprecipitation. Furthermore, we found by immunofluorescence staining that the HBV core-pol fusion protein colocalized with the hepatitis C virus (HCV) core protein in cytoplasm and in lipid droplets. Immunoprecipitation studies showed that the anti-HCV core complex contained the HBV core-pol fusion protein while the anti-HBV pol complex contained the HCV core protein, which supports the hypothesis that the HCV core protein can form a complex with the HBV core-pol fusion protein.

Cytoplasm↗
PubMedDate not supplied

Role of pleural fluid C-reactive protein concentration in discriminating uncomplicated parapneumonic pleural effusions from complicated parapneumonic effusion and empyema.

The aim of this study was to determine whether pleural fluid C-reactive protein (CRP) is useful in distinguishing complicated parapneumonic pleural effusion (CPPE) and empyema from uncomplicated parapneumonic pleural effusions (UPPE). A total of 69 consecutive patients with parapneumonic effusions were enrolled in the study: 29 with UPPE, 29 with CPPE, and 11 with empyema. Concentrations of standard biochemical parameters together with CRP in the pleural fluid were measured using an immunoturbidimetric assay. Pleural CRP was significantly higher in CPPE (11.6 mg/dl) and in empyema (12.2 mg/dl) than in UPPE (3.9 mg/dl). A cutoff value of 8.7 mg/dl for pleural CRP in the diagnosis of CPPE and empyema resulted in a sensitivity, specificity, and area under the receiver-operating characteristic curve (AUC) of 0.80, 0.97 and 0.94, respectively. Traditional lactic dehydrogenase (LDH) > or = 1000 U/L and glucose < or = 60 mg/dl can differentiate CPPE and empyema from UPPE, with the sensitivity, specificity, and AUC achieving 0.75/0.60.1.00/1.00,0.95/0.22, respectively. However, for the detection of CPPE and empyema, the combination of pleural fluid CRP > or = 8.7 mg/dl and LDH > or = 1000 U/L was valuable in achieving a sensitivity, specificity, and AUC of 0.97/1,00/0.95. This study suggests that measurement of pleural CRP can be useful in the workup of patients with a parapneumonic effusion in order to differentiate CPPE from UPPE.

Adult↗