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C Mainguené

Publications and source records attributed to C Mainguené.

At least 19 recordsLinked to original sources
PubMed1997

DNA ploidy by image cytometry in urothelial carcinomas. Comparison of touch imprints and paraffin-embedded biopsies from 31 patients.

OBJECTIVE: To compare DNA content measured by image cytometry from touch imprints and formalinfixed, paraffin-embedded samples in bladder carcinomas. STUDY DESIGN: Thirty-one biopsies of urothelial carcinomas were selected for a prospective study. Imprints of fresh specimens were performed. Cell suspensions were obtained from dewaxed samples by the procedure of Hedley. Sections 7 microns thick were used for carcinoma in situ and small biopsies. The DNA ploidy index was measured on Feulgen-stained slides using an image cytometer. RESULTS: From imprint analysis, seven grade 1 carcinomas (n = 9) were found to be diploid (78%). Nine grade 2 carcinomas (n = 12) exhibited aneuploidy (75%), as did all grade 3 and in situ carcinomas (n = 10). Multiploidy was demonstrated from imprints in four cases instead of the two detected from dewaxed tissue. In 27 cases (87%), G0/G1 peaks obtained from paraffin blocks showed a shift to the left. In five cases (16%), variations in the DNA index were responsible for discrepancies in the DNA ploidy evaluation between fresh imprints and dewaxed samples of the same tumors. CONCLUSION: Image cytometry on Feulgen-stained imprints of bladder biopsies is a simple and reliable procedure for assessing DNA ploidy in urothelial carcinomas, providing great sensitivity for detecting small aneuploid peaks and multiploid tumors. DNA image analysis of touch preparations is especially useful for carcinoma in situ and small biopsies unsuitable for Hedley's technique.

Aged↗
PubMed1996

[Adenomatoid tumors of the uterus. Study of 5 cases with immunohistochemical and ultrastructural confirmation of the mesothelial origin].

Five cases of adenomatoid tumors of the uterus (ATU) are reported. These benign lesions are discovered in 1% of the hysterectomy specimens, performed on 20- to 85-years-old women. Their frequency is certainly underestimated, since ATU have the same macroscopic appearance as leiomyomas. Histologically, ATU are formed by gland-like and pseudovascular lumens, lined by regular, cuboidal or flattened cells, and surrounded by hyperplastic smooth muscle bundles. Immunohistochemical coexpression of cytokeratin and vimentin confirms with a mesothelial histogenesis. The ultrastructural study, showing luminal microvilli and desmosomes supports the hamartomatous development of ATU, corresponding to mesothelial inclusions from the peritoneum into the myometrium. Some giant or diffuse ATU, which are clinically disturbing, are differentiated from carcinomatous or vascular proliferations by frozen section examination. The treatment is made by surgical excision.

Adenomatoid Tumor↗
PubMed1995

Thyroid spindle epithelial tumor with thymus-like differentiation (the "SETTLE" tumor). An immunohistochemical and electron microscopic study.

An intrathyroid primary epithelial spindle-cell tumor with mucous cysts is described in a 9-year-old child. Histologically, this well-circumscribed tumor exhibited a nodular pattern, a prominent spindle cell component with minimal pleomorphism, and well-differentiated mucinous glands within fibrous bands. The spindle cells demonstrated diffuse immunopositivity for cytokeratin and vimentin. Electron microscopy of tissue sections demonstrated that cells contained bundles of cytoplasmic tonofilaments and numerous desmosomes. The light and electron microscopic features and immunohistochemical profile of this tumor were similar to those of recently described thyroid tumors that have been called "SETTLE" tumors (i.e., spindle epithelial tumor with thymus-like differentiation). These uncommon tumors can be considered intrathyroid thymoblastomas and must be regarded as potentially malignant lesions.

Child↗
PubMed1995

[Colonic Histoplasma capsulatum pseudotumor in AIDS. Morphological and immunohistochemical diagnosis of an isolated lesion].

The authors report a case of a 35-year-old man with acquired immunodeficiency syndrome (AIDS) and a left colonic mass with Histoplasma capsulatum (H. capsulatum). The look-up performed looking for disseminated infection was negative. In the absence of positive cultures, the diagnosis was determined morphologically based on the presence of yeast observed by light and electron microscopy. The diagnosis was also verified by positive immunofluorescence using specific anti-Histoplasma antibodies. Gastrointestinal histoplasmosis is a frequent complication of AIDS, particularly in some endemic areas of America. Association with a disseminated mycotic infection is then common. Histoplasmosis is less frequently diagnosed in Europe and isolated involvement of the colon is exceptional. When the mycological study is not performed or is negative, only morphological and immunohistochemical methods are able to establish the diagnosis and eliminate other mycotic diseases occurring during AIDS.

AIDS-Related Opportunistic Infections↗
PubMed1994

Fibroblast-mediated differentiation in human breast carcinoma cells (MCF-7) grown as nodules in vitro.

The growth of cells in 3-dimensional form as nodules in vitro facilitates studies of in vivo cellular interactions. Taking advantage of this technique, human breast carcinoma cells (MCF-7) were co-cultured with stromal fibroblasts isolated from either normal or tumorous breast tissue to study the influence of such fibroblasts on tumor-cell growth and differentiation. Ten days after co-culture of carcinoma cells with fibroblasts from normal tissue at a 1:10 ratio, the size of nodules began to increase and stabilize by day 30 while the fibroblast number decreased and finally disappeared. Concurrently, the carcinoma cells underwent a progressive redifferentiation process which histologically resulted in the appearance of highly developed papillar and tubular structures after 2 months in culture. The production of mucins was further evidence that these cells had undergone differentiation. By contrast, when MCF-7 cells were grown alone or with fibroblasts isolated from a breast carcinoma, the nodules continued to exhibit their characteristic histodedifferentiation properties and did not grow. The re-establishment of a normal epithelial state of differentiation in MCF-7 carcinoma nodules indicates that the phenotypic characteristics of tumor cells are reversible and are influenced or controlled by the stromal environment by which these tumor cells are surrounded or in contact with. Overall, our results open the possibility of exploiting the effects that connective tissue cells have on tumor-cell differentiation for use in prevention and treatment of cancer.

Breast Neoplasms↗
PubMed1994

Lymphokine-activated killer cells induce differentiation in MCF-7 breast carcinoma nodules but not in mastosis nodules maintained in three-dimensional culture.

In order to better understand the interaction between activated lymphocytes and breast carcinoma cells, we studied the degree of infiltration, the membrane contacts established and their cytostatic and cytolytic effects in MCF-7 nodules maintained in three-dimensional culture. A comparison was made with nodules of a nonmalignant, immortalized mastosis cell line. Histological, immunohistochemical and electron microscopical observations were performed as well as DNA synthesis measurements in the two components of the coculture. The lymphokine-activated killer (LAK) cells adhered more frequently to the carcinoma nodules than to the mastosis nodules. They actively penetrated into both of them. The penetration remained peripheral, and only a few cells migrated more deeply. The LAK cells established close cell-to-cell contacts with the two types of nodules, and intercellular gaps were formed: damaged cells could be seen near the activated killer cells. In MCF-7 nodules, a 5-fold inhibition of proliferation occurred, and extensive necrotic zones developed; this was accompanied by a general tendency for glandular redifferentiation. In mastosis nodules, necrosis also developed but no cell differentiation occurred and proliferation was less inhibited (2 times). Interleukin-2 alone enhanced DNA synthesis in mastosis nodules but had no effect on MCF-7 nodules, and no extending necrosis could be seen in both types of nodules. The cytolytic effects of LAK cells combined with their redifferentiating effect in MCF-7 breast carcinoma nodules may be a useful indication for further breast cancer therapy research.

Adenocarcinoma↗
PubMed1993

Anatomic basis of the physiopathology of the epicondylalgias: a study of the deep branch of the radial nerve.

Compression of the deep branch of the radial n. during its passage through Fröhse's arcade in the supinator m. is one of the classical explanations advanced for epicondylalgia. The object of this study was to define the anatomy of the deep branch of the radial n. from its origin up to the origin of the branches to the lateral epicondylar mm. 34 upper limbs were dissected and three segments of the deep branch of the radial n. were distinguished: segment I, from its origin to its entry into the supinator m.; segment II, corresponding to its passage through the supinator m.; and segment III, extending from its exit from the supinator m. to the origin of the nn. to the lateral epicondylar mm. The lengths of these three segments were defined, as well as the level of division of the radial n. in relation to the joint-line, the thickness of the arcade of the supinator m., and the number of nerve branches to the supinator m. It was found that segment III is closely related to the radial head, around which it winds in supination and extension. The three nerve segments thus defined were studied separately microscopically for evidence of lesions. The results were as follows. Segment I: absence of any marked histologic lesions; segment II: marked fibrous thickening of the perineurium and the interstitial connective tissue, replacing certain nerve fibers; segment III: persistence of moderate interstitial fibrosis. These findings appeared constant in all the specimens examined. These results indicate that the deep branch of the radial n. is most affected during its passage through the supinator m.(ABSTRACT TRUNCATED AT 250 WORDS)

Forearm↗
PubMed1993

[Effects of LAK cells activated by IL-2 on MCF-7 human breast cancer cell line maintained in organotypic culture].

Lymphokine Activated Killer (LAK) cells, stimulated by interleukin 2 (IL-2) have a pronounced antitumor effect in the therapy of melanoma and renal cancers. LAK cells were cultivated in presence of the nodules of the human breast adenocarcinoma cell line MCF-7 maintained in organotypic culture to study the interactions between lymphocytes and breast tumor cells. After two days of co-culture, the proliferation of MCF-7 nodules and that of LAK cells was diminished about five folds. The cytotoxic effect of the latter, appreciated by Chrome 51 release was unchanged after the coculture. In histological sections, the penetration of the LAK cells into the MCF-7 nodules was accompanied by an increase of tumor necrosis but also by a glandular differentiation of cancerous tissue. Polarized epithelial cell formations bording neoplasic lumens with intracytoplasmic vacuoles filled with mucus, appeared in the nodules. The immunohistochemistry underlines the presence of T lymphocytes marked by UCHL1 and CD3 antibodies and of Natural Killer (NK) cells marked by IOT10, located between the MCF-7 cancer cells. In electron microscopy, the membrane contacts were tight and were accompanied by the appearance of secondary lysosomes and nuclear alterations. The relatively low infiltration level of the nodules may lead to the supposition that an indirect mechanism will intervene in this dual action of a LAK cells: increase of necrosis, although partially, and development of glandular and functional differentiation.

Breast Neoplasms↗
PubMed1993

[A rare ovarian pseudotumor: massive edema of the ovary].

The authors report a case of massive ovarian edema which declared itself by pain in the abdomen and pelvis and an ovarian mass measuring 13 cm in diameter, occurring in a 22-year-old woman. Since it was not possible to make a diagnosis by any frozen-section examination, histology was carried out on the ovary that had been removed. This showed that the stroma of the ovary had become separated by massive edema preserving the albuginea and the superficial cortex. This case history of massive edema of the ovary shows the characteristics of this ovarian pseudotumour as described in the literature. The principal differential diagnoses of the condition are oedematous fibroma, and myxoma of the ovary. Apart from the fact that torsion of the adnexae can occur in some of these cases, the pathogenesis is still unexplained. When an ovarian tumour is found in a young woman a frozen-section examination must be carried out to make the diagnosis and perhaps avoid oophorectomy, particularly when untwisting a torsion can lead to resorption of the edema.

Adult↗
PubMed1993

[Carcinosarcoma of the breast. Anatomic-clinical study of a case].

The authors report the case of a 66 year old woman, who underwent surgery for carcinosarcoma of the breast. This tumour consisted of an infiltrating ductal carcinoma intermingled with sarcomatous proliferation, resembling malignant fibrous histiocytoma, without evidence of any transition between the two. Carcinosarcomas of the breast are rare tumors. Their definition and their histogenesis are discussed in the literature. Their prognosis is worse than that of metaplastic carcinomas of the breast, particularly spindle cell carcinomas, justifying precise histological classification of these biphasic tumors.

Aged↗
PubMed1990

Virginal breast hypertrophy at puberty; a case report.

Plastic surgery reduction with free nipple graft was performed on an eleven and a half-year-old girl referred to us with massive bilateral macromastia. Hormonal studies revealed no abnormalities. Histopathological examination confirmed the diagnosis of virginal hypertrophy, an infrequent etiology in adolescent macromastia.

Breast↗