Does the composition of propane-1,2-diol alter over time?
Explore the source record for details and available documents.
Cryopreservation↗
Biomedical subjects
C Mallidis
Publications and source records attributed to C Mallidis.
Collection of semen from men in acute phase of spinal cord injury.
In chronic spinal cord injury, semen obtained by assisted ejaculation is usually abnormal. We have assessed electroejaculation early after injury in seven patients. There were no adverse effects. Initial samples contained few or no spermatozoa but as patients emerged from spinal shock, semen improved and five had specimens cryopreserved. Thereafter sperm motility and viability decreased towards the pattern of chronic spinal cord injury by day 16. Cryopreservation was not possible in one patient with many medical complications and another who started electroejaculation 15 days after injury. Semen storage within the first 2 weeks after spinal cord injury is recommended for future fertility treatment.
Acute Disease↗
A simple technique to prevent retrograde ejaculation during assisted ejaculation.
The aim of this study was to develop a technique which would prevent retrograde ejaculation in chronic spinal cord injured (SCI) patients undergoing vibration and electroejaculation procedures. A balloon catheter was used to tamponade the bladder neck in 12 patients who underwent 100 assisted ejaculation procedures. Antegrade ejaculations were collected on all occasions with no incidences of urine contamination and no sperm were seen in post ejaculatory urine. Silicone catheters had minimal effects on sperm motility and viability. All lubricant gels were found to adversely affect sperm quality and were not used.
Adult↗
Immunohistological localization of clusterin in the male genital tract in humans and marmosets.
Clusterin is a multifunctional protein, first described in the reproductive tracts of the rat and the ram. It is produced by several cell types and exists in at least two differentially glycosylated forms. The aim of this study was to extend knowledge of clusterin expression in the primate (human and marmoset) male reproductive tracts by means of clusterin-specific immunohistochemical techniques. In both normal and abnormal testicular tissue, clusterin was found in association with Sertoli cells, lumenal sperm, proacrosomal Golgi complexes, residual bodies, and degenerating germ cells. The major differences observed between the two groups were attributable primarily to morphological differences rather than to clusterin expression specifically. There was no correlation between testicular clusterin content and the cause and severity of spermatogenic disorders. Within normal epididymides, regional differences in clusterin staining similar to those reported in the rat were observed. The seminal vesicles contained large amounts of positive clusterin staining, whereas normal human prostate was completely negative. Low levels of clusterin expression were observed in the marmoset prostate. This study suggests that clusterin is an important and widespread product in the human and marmoset reproductive tracts and is likely to have a role in spermatogenesis.
Animals↗
Fine needle tissue aspiration biopsy of the testis.
OBJECTIVE: To develop a fine needle tissue aspiration biopsy technique that provides sufficient tissue for accurate histological assessment and research purposes. DESIGN: After assessing different needles and developing a method for handling small amounts of tissue, 87 patients underwent fine needle tissue aspiration biopsy. The quantity, quality, and appearance of the tissue was examined and adverse effects of the procedure were noted. Fine needle tissue aspiration and open biopsy samples were compared in 26 patients. Suitability of the tissue for flow cytometry and electron microscopy was also assessed. RESULTS: The 20-gauge, 5-cm Menghini and Turner biopsy needles penetrated the testis more readily and recovered larger quantities of tissue. Adequate samples were recovered from 83% of cases (average of 21.8 tubule sections per testis) with the frequency of recovery and the amount of tissue obtained increasing through-out the study. Some distortions of the tissue were seen but these rarely interfered with the diagnosis. Although many patients suffered slight discomfort during the injection of the anesthetic, no serious complications were encountered during or after the procedure. Comparison of fine needle tissue aspiration and open biopsy diagnoses found either full agreement (56%) or slight differences (38%) in the degree of hypospermatogenesis. CONCLUSIONS: Fine needle tissue aspiration biopsy was found to be a quick, easy, repeatable, and reproducible method of obtaining testicular tissue. No serious adverse effects of the procedure were detected and the diagnoses were found to be comparable to those obtained by open biopsy.
Adult↗
Variation of semen quality in normal men.
To examine the amount of variability in semen analysis results and whether there is any effect of season, 673 specimens provided by seven normal, healthy men (61-205 specimens/subject) over 72-324 weeks were assessed for sperm concentration, ejaculate volume, motility and motility index. Noticeable sample to sample variations were found. The largest proportion of the overall variance was due to within-subject differences, e.g., sperm concentration (54%), ejaculate volume (59%), percentage motility (96%) and motility index (74%). Although changes in semen-analysis results occurred over the year, no consistent trend was seen. No evidence was found to suggest that the differences were due to modifications of the methods employed by the laboratory, or to the change of season.
Adult↗