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C Merlino

Publications and source records attributed to C Merlino.

46 records · Page 3Linked to original sources
PubMedDate not supplied

[Cytotoxic activity of Trichomonas vaginalis supernatants toward cellular monolayers].

In the present paper we analyzed the cytopathogenic activity of 3 recently isolated strains of T. vaginalis on cellular monolayers of human and animal origin. Proliferation of protozoon was accompanied by the progressive and total disintegration of cellular monolayers. Supernatants obtained by incubating cellular monolayers and Trichomonas or Trichomonas alone showed a similar cytopathic effect. A less lytic activity was shown with supernatants containing fetal calf serum. Cytopathogenicity started at 9th hour and was completed at 24th hour. Both this activity and adherence to cells may contribute to the pathogenicity mechanism of T. vaginalis.

Animals↗
PubMedDate not supplied

[Stability of specific antibodies in blood collected on filter paper disks].

Immunoglobulin G (IgG) and M (IgM) stability in serum samples collected on filter paper disks and stored at different temperatures was evaluated. IgG and IgM concentration measured by immunonephelometry was fairly stable for 4 weeks at 4 degrees C, whereas it decreased to about 50% of total at room temperature and at 37 degrees C. By ELISA, no significative loss in antibody activity of IgG was observed after 6 months at 4 degrees C. At 37 degrees C the loss was greater but still acceptable. Antibody activity of IgM decreased more rapidly than that of IgG: IgM were stable at -20 degrees C, whereas had a significative loss of activity after 5 weeks both at 4 degrees C and 37 degrees C. Finally, it is to note that a storage in saturated water vapour seems not to interfere with immunoglobulins stability.

Blood Specimen Collection↗
PubMedDate not supplied

[A fractionation method for human blood for the separation of subclasses of IgG].

A serum fractionation method which permits a good separation among IgG subclasses is proposed. By loading serum on a Protein A-sepharose column, IgG3 are readily recovered as they do not bind to the Protein A. IgG of the other subclasses are eluted, equilibrated at pH 6 and loaded either on a chromatofocusing column, or on an anion exchanger gel. In these conditions IgG4 are retained, and then recovered by lowering the pH, whereas IgG1 and IgG2 pass through the column and can be recovered separately by absorption on a Protein A column and elution with a decreasing pH gradient.

Blood Specimen Collection↗
PubMedDate not supplied

[Fractionation of serum by gel filtration: contamination of IgM fractions by non-specific inhibitors of hemagglutination of the rubella virus].

Rubella virus hemagglutination non-specific inhibitors, in sera fractionated on Bio-Gel A 5 m, elute in IgM containing fractions. In addition, in heparin-MnCl2 treated sera, the same inhibitors display a different chromatographic behaviour on Bio-Gel A 5m, being eluted earlier than IgM. Therefore, in order to avoid false-positive results due to the presence of non-specific inhibitors in IgM containing fractions it is necessary to verify the disappearance of hemagglutination-inhibition activity after 2-ME reduction.

Blood Chemical Analysis↗
PubMedDate not supplied

[Serum fractionation by gel filtration at a high flow rate: use for diagnostic purposes].

High flow rates permit drastic reduction of time required in human serum fractionation by gel filtration giving relatively pure IgM. Fractionation of human serum on Bio-Gel A5m, Superose 6B and Sephacryl S-400, packed in 0.9 X 30 cm columns, at flow rate of 24 cm/h gives IgM fractions employable in diagnosis, in one hour time only.

Acrylic Resins↗