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Biomedical subjects

C Messow

Publications and source records attributed to C Messow.

At least 19 recordsLinked to original sources
PubMed1992

[Morphometric studies of the proximal tubules of the rat kidney considering the effect of the method of killing].

120 male rats belonging to two different strains (Han: WIST, Han: SPRD) and two different weight groups (group I: lighter body weight, group II heavier body weight) were killed on three different methods (decapitation, Nembutal -overdose greater than 250 mg/kg b.w. i.p. less than, exsanguination in Nembutal -anaesthesia greater than 100 mg/kg b.w. i.p.). Subsequently, changes in tubuli contorti I of the left kidneys were examined morphometrically. The mean total area, luminal area and epithelial area were evaluated as well as the mean diameter of the tubular nuclei. Concerning the areas of the proximal tubules only the luminal areas show differences between the Wistar and the Sprague-Dawley strain. The total, luminal and epithelial area in the rats belonging to the weight group II were significantly larger than in the rats belonging to weight group I. With one exception this could be noticed within both strains and all three modes of killing. In both strains and weight groups the rats were killed by Nembutal -overdose or exsanguination showed significantly smaller total, luminal and epithelial areas than the rats that were decapitated. Possible cause heretofore are discussed. Differences between the tubular areas of the rats that were killed by Nembutal -overdose and those that were killed by exsanguination after Nembutal -anaesthesia could not be established. The measurements of nuclei diameters in the proximal tubules did not lead to clearly different results between both strains, weight groups and the various modes of killing.

Animals↗
PubMed1989

Hereditary polycystic kidney disease associated with osteorenal syndrome in rats.

Hereditary polycystic kidney disease (PKD) was seen in Han:SPRD rats and identified as an autosomal, incomplete dominant trait. Homozygous animals died at 3 to 4 weeks of age, while heterozygous males were severely ill or moribund within about 6 months. Heterozygous females developed PKD to a lesser extent than males and survived longer. Renal secondary hyperparathyroidism and osteodystrophia fibrosa were seen in most males but were absent in females. In male rats metastatic calcification was found in kidneys, lungs, glandular and forestomach, blood vessels, and heart. In a few uremic males ulcerative enteritis occurred in cecum and colon. Blood urea nitrogen values were significantly increased in both sexes.

Animals↗
PubMed1989

[Animal welfare relevance and the functional and morphological significance of the killing of laboratory animals].

Euthanasia of experimental animals at the end of an experiment or when an experiment is terminated is relevant for two reasons: firstly, because of ethical reasons and of animal protection, secondly because of scientific evaluation of experimental results. Functional and morphologic alterations have up to now been inadequately observed and considered. Gradually varying different results depend on the different methods of euthanasia. Additionally single parameters of reactions in isolated organs vary depending on the method of euthanasia. These facts have not yet been respected in the discussion of so called alternate methods. The significance of functional and morphologic effects as seen in different methods of euthanasia is discussed with examples.

Animal Welfare↗
PubMed1988

Blood coagulation studies in guineapigs (Cavia porcellus).

Blood coagulation studies were performed on 45 healthy, adult guinea pigs. Additionally thrombelastograms of 30 animals were recorded. Guineapigs revealed short partial thromboplastin times and euglobulin lysis times, but long prothrombin times and thrombin times. Fibrinogen values were within the range of human normal values. Biphasic ADP-induced aggregation of platelets, as occurs in man, was found in 29% of the animals. Short r (reaction time until the beginning of clot formation) and k times (time from the beginning of clot formation until an amplitude of 20 mm) of their thrombelastograms indicate, that whole blood clotting is enhanced in guineapigs. Higher maximum amplitudes in this species suggest a stronger clot stability than in man.

Adenosine Diphosphate↗
PubMed1983

Distribution of glomeruli in the renal cortex of Munich Wistar Frömter (MWF) rats.

The glomerular distribution in the renal cortex of the Munich Wistar Frömter (MWF) rat, a substrain of the Munich Wistar rat selected for superficial glomeruli, was compared to that of a control strain (LEW/Ztm) between 10 and 150 days of age. While there were no significant differences in body growth, kidney weight, or growth of the renal cortex in these two strains, the average diameter of Bowman's capsule and of the glomerular tuft was significantly larger (about 12%) in rats of the MWF strain which were older than 60 days. In histological sections of kidneys of MWF rats in all age groups, many glomeruli in direct contact with the renal capsule were found; thus, this developmental step must have taken place before 10 days of age. The appearance of superficial glomeruli was due to a shift of glomeruli to a position just under the renal surface, the subcapsular zone or 'cortex corticis', which is normally free of glomeruli. For the rest of the renal cortex there were only slight differences in glomerular density and in total number of glomeruli per section between these two strains. These results lead us to suppose that superficial glomeruli belong to a special nephron type; thus, these glomeruli are not enveloped by parts of the proximal tubule.

Animals↗
PubMed1982

Urethral plug--a new secondary male sex characteristic in rat and other rodents.

The plug is an eosinophilic mass, partly homogenous and partly porous, filling the proximal urethra in rats and occasionally extending into the bladder. Its average weight in 131 adult rats was 0.063 g. These plugs are normally present in the urethra of adult male rats, and this seems to be the case for all laboratory Muridae and Cavidae. The absence of a plug in an adult male may be a sign of abnormality associated with failing health. There is an interesting similarity between the amino acid composition of the content of seminal vesicles, that of the urethral plug, and that of the copulatory vaginal plug in female rodents.

Animals↗
PubMed1980

Sex differences in kidney morphology and glomerular filtration rate in mice.

The relative kidney weight (1.44% greater than 1.15% greater than 1.07%) and the GFR (44 greater than 39 greater than 35 ml/min . m2) are higher in adult males than in adult females or in orchiectomized males. In contrast, GFR based on kidney weight is smallest in healthy males (1.02 less than 1.22; 1.22 ml/min . g). Only the cortex tissue seems to be increased in males, because its width (1.67 mm) is greater than in females (1.26 mm), while medullary width is identical (2.4 mm) in both sexes. Enlargement of the cortex in males can be accounted for by the nonglomerular tissue, because glomerular diameters (120 micrometers) do not differ and the number of glomeruli in representative sections is lower than in females. Cuboidal cell transformation of the outer layer of Bowman's capsule was observed in 60% of the glomeruli of the males and in 5% of the females. It might be an indicator for hyperplasie of the proximal tubular cells in the males.

Animals↗