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Biomedical subjects

C Micouin

Publications and source records attributed to C Micouin.

At least 19 recordsLinked to original sources
PubMed1990

A human monoclonal IgM with autoantibody activities against heparan sulphate and the mitotic spindle.

A monoclonal IgM kappa from a patient with Waldenström's macroglobulinaemia (IgM-Rod) was found to react at temperatures below 28 degrees C with all tissue basement membranes and the cell coat of non-haematopoietic cells. IgM-Rod antibody was directed against heparan sulphate side chains of heparan sulphate proteoglycans as shown by binding in a solid-phase ELISA to heparan sulphate glycosaminoglycans but not to other purified subcomponents of the extracellular matrix; and by specific inhibition of the observed reactivity by heparitinase treatment. IgM-Rod showed cross-reactivity by indirect immunofluorescence with an as yet unidentified structure expressed in the nucleus during cell division and becoming associated with the mitotic spindle apparatus. The co-existence of both binding activities for heparan sulphate and nuclei determinants in the same IgM molecule was deduced from adsorption-elution experiments and from the inhibitory effect of a mouse monoclonal anti-idiotypic antibody directed against the paratope of IgM-Rod.

Antibodies, Anti-Idiotypic↗
PubMed1989

Establishment and characterization of a granulocytic subclone (UM 384) from the monoblastic cell line U 937.

The human cell line U 937 spontaneously expresses monocytic maturation and can be induced into macrophage-like cells when treated with retinoic acid, sodium butyrate, or 2,3-O-tetra decanoylphorbol-13-acetate. We have selected a subclone, designated UM 384, that expresses granulocytic characteristics and can be induced to mature to granulocytes after exposure to retinoic acid, actinomycin D, and dimethylsulfoxide, and to monocyte-like cells when treated with sodium butyrate and phytohemagglutinin-stimulated leukocyte-conditioned medium. These cells retain the same constitutive markers as the parent line including histocompatibility leukocyte antigens and karyotype but share numerous chromosomal abnormalities, mainly t(X;8) (p21;q12).

Antigens, Differentiation↗
PubMed1987

Human chronic myeloid leukemic cell line with positive Philadelphia chromosome exhibits megakaryocytic and erythroid characteristics.

A cell line (LAMA-84) has been established from the blood of a patient with chronic myeloid leukemia in acute phase. LAMA-84 cells retained the patient's chromosome abnormalities, i.e., triplication of all chromosomes except chromosome 18, the presence of Philadelphia (Ph) chromosome in 4-5 copies, and the presence of chromosome markers. LAMA-84 cells have morphological features of undifferentiated blast cells, but analyses have indicated that they belong to the megakaryocytic lineage; platelet peroxidase (PPO) was found in 8.5% of cells; LAMA-84 cells reacted spontaneously with poly- and monoclonal antibodies against the platelet glycoproteins (GP) IIb, IIIa, and the GPIIb/IIIa complex, whose presence was confirmed by crossed immunoelectrophoresis. LAMA-84 cells lack the membrane characteristics of lymphoid and mature granulocytic cells but do, however, react with certain antibodies to immature myeloid cells. Furthermore, they are positive with an antiglycophorin antibody, and contain alpha- and gamma-globin mRNA, thus demonstrating erythroid marker expression. Thus LAMA-84 is a tripotent, megakaryocytic, erythroid, and granulocytic cell line. The megakaryocytic and erythroid markers were enhanced by the addition of DMSO, butyrate, TPA, and hemin. The LAMA-84 cell line represents an interesting tool for the study of megakaryocytic and erythroid differentiation and the mechanisms of neoplastic growth.

Adult↗
PubMed1984

Monoclonal IgM with anti-muscle tissue activity in a patient with Waldenström's macroglobulinaemia: secretion of identical immunoglobulin by the established lymphoid cell line.

The monoclonal IgM kappa protein from a patient with Waldenström's macroglobulinaemia displayed antibody like activity against muscle and alpha-actinin. An Epstein-Barr virus associated lymphoid cell line, spontaneously established from blood lymphocytes, has been continuously secreting an immunoglobulin of the same isotype, isoelectric focusing spectrotype and reactivity; the cell line is probably leukaemic in origin since all cells display the translocation t (1; 19) and are aneuploid. We suggest these two monoclonal immunoglobulins have a common malignant origin.

Aged↗
PubMed1983

Herpes simplex virus glycoprotein D: human monoclonal antibody produced by bone marrow cell line.

Normal bone marrow cells from a donor positive for herpes simplex virus were transformed with Epstein-Barr virus. The resulting lymphoblastoid cell line has secreted immunoglobulin G1 of the kappa type continuously for 2 years. This immunoglobulin, detected both on the cell surface and in the cytoplasm, reacts with cells infected with herpes simplex virus. It defines an antigen that comigrates with the 55-kilodalton glycoprotein D of herpes simplex virus type 1 and neutralizes the infectivity of herpes simplex viruses 1 and 2.

Aged↗
PubMed1981

Heterogeneous distribution of bullous pemphigoid antigen during hair development in the mouse.

The appearance and localization of one of the basement membrane constituents, the bullous pemphigoid antigen, were studied during the development of hair follicles in the mouse. The analysis was performed, with the indirect immunofluorescent method, on frozen sections of dorsal skin, between 14 days of gestation and 10 days post partum. Specific labelling was restricted to the epidermal-dermal junction (EDJ). The earliest positive reaction was seen in 15-day embryos as a continuous underlining of the EDJ. At later stages throughout embryonic development and up to 10 days post partum, in hair rudiments labelling of the EDJ was interrupted. In particular, the EDJ along the underside of hair placodes and nodules, the lower half of follicles at the bulb and hair cone stage, as well as around the dermal papilla was not or very faintly labelled. By contrast, in the upper portion of follicles and interplumar skin, the EDJ was brightly labelled. It is concluded that bullous pemphigoid antigen is absent or scarce in zones of morphogenetic activity, where epidermal-dermal interactions are supposedly exerted, whereas it is present in zones of histological stability.

Animals↗
PubMed1980

Monoclonal cryoimmunoglobulin with anti-cytomegalovirus activity associated with T cell chronic lymphocytic leukaemia.

A patient with chronic T cell lymphocytic leukaemia developed a monoclonal immunoglobulin (IgG3 kappa = 14 g/l) which was in part cryoprecipitable. At the same time, a subclinical CMV infection occurred which was associated with a neutropenia and thrombocytopenia, and which led to a rise in anti-CMV antibodies. The F(ab')2 fragment of IgG3 kappa, obtained by enzymatic cleavage, was examined for several antiviral activities and it was found to have a strong anti-CMV activity using the immunofluorescence test with anti-kappa conjugate. This is one of the few examples of a cryoglobulin with specific antiviral activity. The leukaemia, possibly together with immunosuppressive therapy, may have been responsible for the uncontrolled proliferation of the clone producing the cryoimmunoglobulin.

Aged↗
PubMed1980

[Rheumatoid factors : a cause of non-specific results in IgM antitoxoplasma fluorescent tests (author's transl)].

This paper confirms the finding that rheumatoid factor (RF) can cause non-specific fluorescence in fluorescent-antibody tests for toxoplasmosis carried out with IgM antiserum (Remington test). There is a 20 to 25 p. cent risk of such error, depending on the conditions under which the test is read. Elimination of the risk by the subjection of all sera to preliminary absorption, as proposed by Camargo et al., does not seem justified as the proportion of positive Remington tests is very low in France (approximately 1,5 of every 1000 sera tested) and the work involved would be wasted is most cases. What is proposed here is to tackle the problem at the other end by taking every serum with a positive response to the Remington test, testing it for RE, absorbing it on heat-aggregated human globulin of placental origin, and determining its IgM toxoplasma antibody titre again.

Antibodies↗
PubMed1977

[Placental origin of the progesterone binding protein in the pregnant guinea pig; immunohistoligical study].

The origin of the high affinity progesterone binding protein (PBP) occurring during pregnancy in the guinea pig serum has been investigated. An indirect immunofluorescence technique has been developed using a specific antiserum raised in rabbits. An accumulation of PBP-like immunofluorescent material was detected in the trophoblastic syncytium of the placenta tissue, whereas all other examined pregnant guinea pig organs gave negative reactions. It is concluded that this placental accumulation of immunoreactive PBP-like material may express a placental biosynthesis of this glycoprotein.

Alpha-Globulins↗
PubMed1977

Quantitative study of T and B lymphocytes in Hodgkin's disease.

Peripheral blood lymphocytes forming E rosettes in the presence of sheep red blood cells and those bearing surface immunoglobulins (SIg) have been studied quantitatively as an evaluation of T and B lymphocytes in Hodgkin's disease. 62 patients were investigated, 21 of whom before any treatment. It appears that the lymphocytes forming E rosettes are significantly lower in percentage in 86% of the patients and in absolute count in 65%. SIg bearing lymphocytes are elevated in percentile in 61% of the cases, but the absolute count is normal in 50% of the patients and elevated in 30% only. At diagnosis the T/B lymphocytes equilibrium is modified in 13 among 21 patients but, after the initial treatment of the disease, the ratio is modified in 90% of the patients in complete remission and remains unchanged for years even in the absence of relapse or immnunosuppressive treatment. It is suggested that SIg + lymphocytes from the peripheral blood are actually B lymphocytes and not anti-T-antibody coated T lymphocytes or antigen-antibody lacking of membrane markers, which are numerous in one third of the investigated patients, might be T lymphocytes with qualitative abnormality.

Adult↗
PubMed1977

A human immunoglobulin in myeloma protein with anti-gastric parietal cell autoantigen activity.

A previously characterised IgA Kappa myeloma protein was isolated and purified. The (Fab) alpha fragments of this immunoglobulin were obtained. The IgA and Fab fragments reacted in vitro with gastric parietal cells (GPC) using animal gastric sections and with smooth cytoplasmic membranes obtained from rabbit fundus (gastric) mucosa. This was seen macroscopically and by light and electron microscopy. Evidence is thus provided which supports the concept that this homogeneous immunoglobulin is a typical monoclonal autoantibody.

Autoantibodies↗
PubMed1975

[Anti-nuclear antibodies in collagen diseases].

The authors sought serum antinuclear antibodies by the indirect immunofluorescent method in 3,260 patients, and found these antibodies in 293 of them. 90 patients had a level equal or greater than 1/50. Among the latter, 54 had some form of collagen disease. The following facts are emphasized: -- antinuclear antibodies are always present in lupus erythematosus. Their levels fall under the influence of treatment when nephritis occurs; -- their frequency is greater (13 cases out of 16) during scleroderma, with often a hazy appearance; -- they are lower during rheumatoid arthritis (23% of cases had a significant level), and their presence is not a sign of worse prognosis; -- they were absent in other forms of collagen diseases.

Adult↗
PubMed1975

Mixed IgG-IgM cryoglobulinemia with glomerulonephritis. Immunochemical, fluorescent and ultrastructural study of kidney and in vitro cryoprecipitate.

Comparative studies of renal biopsy specimens and in vitro cryoprecipitate were carried out in a patient with mixed immunoglobulin G (IgG)-immunoglobulin M (IgM) cryoglobulinemia associated with glomerulonephritis. The IgM isolated from the cryoprecipitate was an antibody with anti-IgG activity. Proliferative endocapillary glomerulonephritis was found in the kidney, with large amorphous deposits in the capillary walls. On immunofluorescent examination, these deposits contained IgG and C3. Ultrastructural studies of both cryoprecipitate and glomerular deposits revealed unusual structures designated as "cylindrical or annular bodies." The morphologic characteristics of these bodies were exactly the same in the kidney and in the cryoprecipitate. These findings suggest an identity between the glomerular deposits and the circulating cryoglobulin, supporting the hypothesis that the glomerulonephritis reported here is an immune-complex disease.

Aged↗