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Biomedical subjects

C Montero

Publications and source records attributed to C Montero.

At least 91 records · Page 5Linked to original sources
PubMed1978

Immunocytochemical and histochemical studies on intestinal epithelial cells producing both lysozyme and mucosubstance.

The simultaneous demonstration of lysozyme using the unlabelled antibody enzyme method and mucosubstances by staining with the periodic acid-Schiff (PAS) and Alcian Blue pH 2.5 techniques has led to the identification of a new subpopulation of mucus-producing cells containing lysozyme in small intestinal specimens from normal rats and from patients with inflammatory bowel diseases. These cells, containing both mucosubstances and lysozyme, are located in the lateral walls of the crypts of Lieberkuhn, but can occasionally be found also in or near the tips of the villi. The specific staining for lysozyme was observed in the apical and/or basal cytoplasm of these mucus-producing cells and was readily detected in sections counterstained for mucosubstances with Alcian Blue. The localization of these mucus-producing cells was similar both in the normal rat and in the pathological human specimens. Absorption controls and controls where a non-immune serum was substituted for the specific antilysozyme serum confirmed the specificity of the lysozyme localization.

Animals↗
PubMed1978

[Histochemical demonstration of acetylcholinesterase activity applied to the study of human biopsies. I. Rectal suction biopsy in the diagnosis of congenital megacolon].

The application of the histochemical demonstration of AChE activity to the diagnosis of congenital megacolon (Hirschsprung's disease) is discussed, both from the point of view of the technical aspects as well as the interpretation of the results obtained, using the method of Karnovsky and Roots, as modified by El Badawi-Schenk, applied to rectal suction biopsy. The authors adhere to the original ideas of Meier-Ruge et al., that the diagnosis of Hirschsprung's disease can be done solely with the evaluation of the AChE positive parasympathetic network of the lamina propria, with the additional consideration of the submucosal plexus to include cases of hypoganglionar megacolon, where that plexus is clearly diminished. The use of phase contrast is also emphasized.

Acetylcholinesterase↗
PubMed1976

Immunocytochemical identification and localization of immunoglobulin A within Paneth cells of the rat small intestine.

Light microscopic immunocytochemistry was used to identify Paneth cells by their lysozyme content and to detect immunoglobulin antigens within a subpopulation of these cells. Antisera specific for the heavy chains of rat or human immunoglobulin A and for immunoglobulin light chain antigens produced specific staining of rat Paneth cells. The distribution of immunoglobulin staining varied between adjacent Paneth cells in the same crypt and between Paneth cells in adjacent crypts, as well as between Paneth cell populations of different animals. No staining of rat Paneth cells was detected using antisera specific for the heavy chain of immunoglobulins G or M. The specific staining of Paneth cells for immunoglobulin A and light chain antigens was blocked by absorption of each antiserum with its respective purified antigen. Absorption of these antisera with purified rat lysozyme did not affect staining and thereby eliminated the possibility of immunologic cross-reactivity between lysozyme and immunoglobulin antigens. It is suggested, in light of current concepts of Paneth cell function, that the immunoglobulin staining of Paneth cells may reflect their ability to phagocytize immunoglobulin A-coated microorganisms or immune complexes containing immunoglobulin A.

Animals↗
PubMedDate not supplied

Laparoscopic and histologic findings in patients with the human immunodeficiency virus.

Hepatic dysfunction frequently develops in patients infected with the human immunodeficiency virus (HIV). This retrospective study was undertaken to determine the laparoscopic and histologic findings in a group of HIV-seropositive patients with or without the acquired immune deficiency syndrome (AIDS). Fifty-four patients, 44 with AIDS and 10 HIV-positive, underwent laparoscopic examination and visually guided biopsies for the assessment of clinical or biochemical evidence of liver injury. Significant abnormalities were detected in 31/44 (70%) AIDS patients and 3/10 (30%) HIV-positive patients. Overall, specific laparoscopic findings were described in 25/54 (46%). The most common findings were peritoneal involvement, massive intra-abdominal adhesions, focal lesions of the liver or spleen, and diffuse nodularity of the liver; these were usually related to opportunistic infections or neoplasms such as non-Hodgkin's lymphomas and Kaposi's sarcoma. No procedure-related deaths occurred. Laparoscopy is a safe and accurate method to detect underlying disease in a selected population of HIV-seropositive patients.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

[Benign fibrous mesothelioma: report of 8 cases].

Benign fibrous mesothelioma (BFM) is a primary, isolated tumor of the pleura. In 80% of patients the tumor originates in the visceral pleura. BFM is rare and localized malignant mesothelioma, whose prognosis and treatment is significantly different, must be considered as a differential diagnosis. We report 8 cases of BFM excised by thoracotomy. In 6 asymptomatic patients, diagnosis was based on radiological images. One patient with a large tumor suffered dyspnea, acropachia and hypertrophic osteoarthropathy. The last patient experienced long-lasting chest pain even though the tumor was small (3 x 3 x 1 cm). Diagnosis was before thoracotomy in 3 cases, 2 by punch biopsy (tru-cut) and the other by thoracoscopy. The results of pleural fluid analysis were nonspecific in 2 of the 3 cases in which pleural effusion was present. Thoracotomy allowed removal of the entire tumor in all patients.

Aged↗
PubMedDate not supplied

Chronic calf pain in athletes due to sural nerve entrapment. A report of 18 cases.

We retrospectively analyzed the charts of 13 athletes (18 limbs) who had sural nerve entrapment localized in the passage of the nerve through the superficial sural aponeurosis. There were 11 men and 2 women (average age, 43 years; range, 31 to 59). All patients reported chronic calf pain that was exacerbated during physical exertion. Delay to diagnosis averaged 9 months (range, 5 to 24). Tenderness in the calf was identified along the course of the sural nerve in all cases. In 10 patients (15 limbs) electrodiagnostic testing before surgery was positive. After failure of nonoperative treatment, surgery was conducted under local anesthesia. Neurolysis was performed by incising the superficial sural aponeurosis and the fibrous band in it through which the nerve passes. The results of the operation were evaluated in terms of residual symptoms, ability to return to the former sport, and degree of patient satisfaction. A final follow-up examination was performed an average of 14 months (range, 6 to 30) after the operation. The final result was excellent in 9 limbs (2 bilateral), good in 8 limbs (2 bilateral), and fair in 1 case. The differential diagnosis of sural nerve entrapment in athletes is discussed. Increase in sural muscle mass or development of local fibrous scar tissue compromised the sural nerve in its course through the unyielding and inextensible superficial sural aponeurosis.

Adult↗
PubMedDate not supplied

[Feeding pattern of Triatoma rubrovaria (Blanchard, 1843) (Hemiptera, Triatominae) in peridomiciliary habitats, of a rural area of Uruguay].

It has been studied the feeding patterns of Triatoma rubrovaria (wild triatominae bug, and intradomiciliary secondary vector of T. cruzi) in a rural area of "La Bolsa" (Department of Artigas). Agar double diffusion test were used for analyze the blood meal, from 120 insects, which were confronted to 13 antisera. It has 251 identify blood meals, and the most frequently was mammalian host (73%), but it has a variable source of feeding (mammals, birds, reptiles and cockroach), including humans rate 8% of the total. The identify of haemolimphe as feed, place this triatominae bug as an intermediary vector between predator and haematophagous. The most usual mammals are dasypodides and bovines. The frequence of human blood meal near the dwellings, shows its potentiality as vectors. Therefore, the peridomicile is an area of interaction between, domestic, wild and sinantropic host. The trypanosomic infection's rate at peridomiciles was lesser those at wild areas.

Animals↗
PubMedDate not supplied

Ecology of Triatoma rubrovaria (Hemiptera, Triatominae) in wild and peridomestic environments of Uruguay.

This paper describes population structure, spatial distribution and habitat selection of wild and peridomestic populations of Triatoma rubrovaria (Blanchard, 1843). Field studies were carried out at Las Piedras and La Bolsa in the Northern Department of Artigas, Uruguay. A semicircular sampling area, divided in seven or eight triangular sectors was sequentially examined from October 1990 to November 1991. At Las Piedras (typical wild habitat) 1063 T. rubrovaria bugs were collected from 84% of the rocky outcroops ("pedregales"). Abundance varied by season peaking in October-November (spring). Throughout the year, most of the population was made up of third, fourth and fifth instar nymphs; adults were found from October to March. In the peridomestic environment of La Bolsa, however T. rubrovaria was less common and showed a more irregular instar distribution. Colonized ecotopes, were those close to houses. In both sites T. rubrovaria was mainly associated with the geckonid Homonota uruguayensis and the cockroach Blaptica dubia.

Animals↗
PubMedDate not supplied

Extradural spinal meningiomas with intrathoracic extension. Report of two cases.

Two cases of extradural spinal meningiomas with intrathoracic extension are presented. Both cases were operated on previously for spinal epidural meningiomas. The first one showed predominantly intrathoracic growth and the second, dural invasion with protrusion through the root exit. In these cases meningiomas arose from the thoracic root exit. The surgical procedure is discussed. We advise postoperative X-ray therapy in these tumors and a long term follow-up of the patients.

Adult↗