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Biomedical subjects

C Neri

Publications and source records attributed to C Neri.

41 records · Page 3Linked to original sources
PubMedDate not supplied

Determination of morphine in the hair of heroin addicts by high performance liquid chromatography with fluorimetric detection.

A procedure has been presented for the quantitative determination of morphine contained in the hair of heroin addicts, by means of heat-acid hydrolysis, pre-column dansyl derivatization, straight phase liquid chromatography, and fluorescence detection. External standardization was used. Intra-assay and day-to-day variation coefficients were 5.6 and 7.8%, respectively (n = 10), when hair containing 1 ng/mg of morphine was assayed. Hair samples of 22 heroin addicts showed positive results in the range 0.08 to 15.7 ng/mg. No false positive results were found in 20 control subjects. A close correlation was shown between high performance liquid chromatography and radioimmunoassay results (y = 0.97x + 0.26)(r = 0.997, n = 15). Morphine hair content results significantly correlated with the grade of heroin use roughly estimated by means of serial determinations of morphine in urines during the last months before hair sampling.

Adolescent↗
PubMedDate not supplied

Distinct effects of gonadotropin-releasing hormone analogs and 4-hydroxytamoxifen on pS2 mRNA expression with respect to cell proliferation in MCF-7 breast cancer cells.

Gonadotropin-releasing hormone (GnRH) analogs and antiestrogens display direct antiestrogenic effects on the proliferation of hormone-sensitive breast cancer cells. This study aimed to determine whether growth inhibition of 17 beta-estradiol-stimulated MCF-7 breast cancer cells by the agonist D-Trp6 GnRH, the GnRH antagonist BIM 21009C and 4-hydroxy-tamoxifen (OHT) respectively occurred through alterations of the estrogen receptor (ER)-mediated intracellular pathway. The pS2 mRNA expression is primarily dependent on activated ER in MCF-7 cells, and pS2 protein could act as a growth factor. Drug effect on pS2 mRNAs were qualitatively compared to those on the cell cycle. Unlike OHT, GnRH analogs did not suppress the 17 beta-estradiol-induced pS2 mRNA expression whilst the cell cycle was blocked. The pS2 mRNA expression was induced by D-Trp6 GnRH alone without effect on the cell cycle. The outcome of our study is double. Firstly, GnRH analogs are distinct from OHT as regards their effects on the ER-mediated intracellular pathway. Secondly, pS2 mRNA expression is not strictly related to MCF-7 cell proliferation, suggesting that pS2 protein has a function other than that of critical growth regulator.

Antineoplastic Agents↗
PubMedDate not supplied

Unthinkability and psychosomatic symptoms.

W. R. Bion (1952) has pointed out a connection between alterations in the development of the "apparatus for thinking thoughts" and psychosomatic symptoms. Many authors have used this insight, from their own points of view, as a basis for describing this deficiency in thought and in the capacity to formulate images related to the development of psychosomatic symptoms (Krystal & McDougall 1979; Segal, 1950, 1958). This paper applies this hypothesis to a clinical case in which special emphasis is given to the symbolic deficiency, its effect on transference-countertransference, and its relation to falsification, "hyposymbolization," and to a specific phenomenon that could be called "hypersymbolization," in which many meanings are embodied in the same symbol.

Countertransference↗