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Biomedical subjects

C Nicholls

Publications and source records attributed to C Nicholls.

14 recordsLinked to original sources
PubMed1991

Waterborne outbreak of Campylobacter jejuni in Christchurch: the importance of a combined epidemiologic and microbiologic investigation.

Campylobacter is a common cause of gastroenteritis in New Zealand; however, the source of infection usually remains unknown. Reports of two cases of Campylobacter jejuni enteritis at a camp and convention centre near Christchurch were investigated. Through interviews of persons living at or attending the camp, 42 additional cases were identified. Epidemiologic and microbiologic data strongly suggested the water supply as the source of infection. The combined epidemiologic and microbiologic investigation was useful in quickly defining the magnitude and source of the outbreak, allowing for rapid implementation of control measures.

Campylobacter Infections↗
PubMed1990

The construction of Bacillus thuringiensis strains expressing novel entomocidal delta-endotoxin combinations.

Using our recently reported method of electroporation to transform Bacillus thuringiensis [Bone & Ellar (1989) FEMS Microbiol. Lett. 58, 171-178], cloned B. thuringiensis entomocidal delta-endotoxin genes have been introduced into several native B. thuringiensis strains. In many cases the resulting transformants expressed both their native toxins and the cloned toxin, producing strains with broader toxicity spectra. The introduction of the var. tenebrionis toxin gene into B. thuringiensis var. israelensis resulted in a strain with activity against Pieris brassicae (cabbage white butterfly), an activity which neither parent strain possesses. We discuss further the possibility of synergism and also the problems associated with introducing cloned DNA by this method.

Bacillus thuringiensis↗
PubMed1985

Genetic linkage analysis of epidermolysis bullosa dystrophica, Cockayne-Touraine type.

Genetic linkage relationships between 27 informative marker loci and the locus for epidermolysis bullosa dystrophica, Cockayne-Touraine type (EBD-CT), were examined in a single large kindred. Linkage could not be demonstrated to any of the marker loci, further adding to the exclusion map for EBD-CT. The dominant forms of EBD so far delineated by clinical criteria and electron microscopy remain genetically undefined in terms of loci and allelism. Further investigation will be undertaken using restriction fragment length polymorphisms mapped to regions outside the existing exclusion map.

Adolescent↗
PubMed1983

A search for linkage in families with fragile sites.

Linkage relationships to unassigned and provisionally assigned genetic markers were examined from 53 families segregating for various fragile sites. Fragile sites were at Xq27, 2q13, 6p23, 9p21, 9p32, 10q23, 10q25, 11q13, 11q23, 12q13 and 16p12. No new assignments were made but extensive exclusion data are presented for the markers F13A, F13B, CHE2, K, TF, GPT, C3 and Lu arising from linkage comparisons with fragile sites and other genetic markers. Exclusions of at least 10 cM involving fragile site markers were made for F13A (6p23, 9q32, 10q25), F13B (10q23, 10q25, 16p12), CHE2 (16p12), K (10q23), TF (10q23, 10q25, 11q13), GPT (10q23, 10q25, 11q13), C3 (10q23, 10q25) and Lu (10q25, 11q13).

Chromosome Banding↗
PubMed1982

Distribution of the GC (group-specific component) subtypes in cord bloods and blood donors.

The common allele, GC/1, was subtyped into GC*IF and GC*IS by isoelectric focusing, increasing the proportion of heterozygotes from 0.42 to 0.58. In a series of 200 blood donors and 200 cord bloods the allele frequencies were found to be: GC*IS = 0.59, GC*IF = 0.11, GC*2 = 0.30; and GC*IS = 0.54, GC*IF = 0.16, GC*2 = 0.30, respectively. These values were similar to frequencies obtained in studies of other Caucasian populations. The average probability of detecting non-paternity for this population is increased from 17% to 30% after subtyping GC 1.

Alleles↗
PubMed1982

Distribution of six TF C (transferrin) subtypes in cord bloods and blood donors.

Isoelectric focusing was used to subtype the common TF C phenotype into C1, C2 and C3, increasing the proportion of heterozygotes from virtually zero to 0.31. Five of the six subtypes of TF C were found in a series of 200 blood donors and 200 cord bloods. The frequencies of the three alleles were: TF*C1 = 0.80, TF*C2 = 0.17 and TF*C3 = 0.03 from cord bloods; and TF*C1 = 0.84, TF*C2 = 0.13 and TF*C3 = 0.03 from blood donors. These frequencies were comparable with estimates from other Caucasian populations. The average probability of detecting non-paternity for this population is increased to 11% by subtyping C into C1 and C2, and further increased to 15% by subtyping C into C1, C2 and C3.

Alleles↗