Biomedical subjects

C Olson

Publications and source records attributed to C Olson.

At least 91 records · Page 5Linked to original sources

PubMed1977

B-lymphocytes and T-lymphocytes in three types of bovine lymphosarcoma.

Lymphoid cells of peripheral blood, lymph nodes, and thymus from clinically normal cattle, cattle infected with bovine leukemia virus (BLV), and cattle with lymphosarcoma were characterized for T- and B-cell surface markers. B-cells were detected by the erythrocyte-antibody-complement (EAC) rosette test and the surface immunoglobulin (sig) immunofluorescence assay. Peripheral blood from BLV-infected cattle had a higher than normal percentage of B-cells by both EAC rosette and sig immunofluorescence assays. Lymphoid cells from tumorous lymph nodes of cattle with the adult type of lymphosarcoma had a higher than normal percentage of sig-bearing cells, but in the same cell preparation the EAC rosette-positive cells were fewer than sig-positive cells. T-cells were detected by the erythrocyte rosette test. The percentage of T-cells by this test in lymph nodes of adult type lymphosarcoma was lower than that in normal cattle. A distinctly lower than normal percentage of lymphocytes could be characterized as either B- or T-cells in lymph nodes thymus, and peripheral blood from the calf type and thymic type of lymphosarcoma.

Animals↗

PubMed1977

Tumor-associated antigen in bovine and ovine lymphosarcoma.

Specific tumor-associated antigens were found on the membrane and in the cytoplasm of lymph node cells and peripheral blood lymphocytes (PBL) from cattle and sheep with lymphosarcoma by immunofluorescence tests. Materials from 15 cattle with the adult form of lymphosarcoma were examined. Cytoplasmic antigen was detected in fixed tumor cells from all 15 cases and in PBL from 9 cases tested. Membrane antigen was detected in living cells from 10 of the cases tested. In 3 calf-type cases, cytoplasmic antigen was found in a few (1 to 3%) of the tumor cells, while 1% of the cells from 2 thymic cases had cytoplasmic tumor antigen. In 15 cattle infected with bovine leukemia virus (BLV) but with no evidence of tumor, PBL from 3 cattle had the tumor-associated antigen in the cytoplasm. Negative results were obtained with similar tests done with 9 normal cattle that had no detectable BLV or BLV antibody. Cells from tumors induced with BLV in 5 sheep also had cytoplasmic antigen and membrane tumor-associated antigen. Tumor-associated antigen was found in PBL from 1 or 7 BLV-infected sheep with no clinical evidence of tumor. Similar tests were negative on 4 normal sheep.

Animals↗

PubMed1977

Fetal infection with bovine leukemia virus in sheep.

Several sheep fetuses were thymectomized, and their tails were removed at 58 to 65 days of gestation for tissue culture. Bovine leukemia virus (BLV) antigens were detected in serial culture of tissues from fetuses whose dams and sires were both BLV positive. However, no BLV antigens were detected in serial cultures of tissues from fetuses whose dams were negative but whose sire was positive. Precolostral serums from 3 of 16 neonatal lambs, whose sire and dams were both BLV positive, were BLV antibody positive. Thus, BLV may be vertically transmitted from a positive dam to her lamb via the placenta and/or germinal cells but not from sire to lamb.

Animals↗

PubMed1977

Bovine leukemia virus-associated antigens in lymphocyte cultures.

Short-term lymphocyte cultures from bovine leukemia virus (BLV)-infected cattle were tested for BLV-associated antigens at various times after incubation. Several immunologic methods were used, including fluorescent antibody tests, immunodiffusion, and radial immunodiffusion. Antigens were not detected in uncultured lymphocytes. The BLV-associated antigens were detected as early as 3 hours, with maximum antigen production occurring at 18 to 24 hours after incubation. These results indicate that culturing of lymphocytes in vitro is necessary for the expression of the virus.

Animals↗

PubMed1976

Effect of pasteurization and heat treatment on bovine leukemia virus.

Four sheep inoculated with mixtures of bovine leukemia virus (BLV) and milk that was treated by a simulated high-temperature short-time pasteurization procedure did not become infected with BLV or develop tumor. Four sheep inoculated with unpasteurized BLV-mil mixtures became infected with BLV and 3 died with tumor at 15, 21, and 27 months, respectively, after inoculation. Fluid from a BLV-infected cell culture was heated to 56, 60, 65, and 73 C for 1/2 minute and 1 minute (in separate trials) and transferred to noninfected cells. Culture fluid heated to 60 C and higher did not infect the cells.

Animals↗

PubMed1976

Lack of infectivity of bovine leukemia (C-type) virus to rats.

Sprague-Dawley rats were given ip injections of bovine culture and sheep cultures of bovine leukemia virus (BLV) and Gross passage-A leukemia virus [MuLV(G)]. Sera were tested to BLV antigens. BLV did not induce tumors in Sprague-Dawley rats, but the rats were susceptible to MuLV(G) at low doses.

AKR murine leukemia virus↗

PubMed1976

Properties of two isolated antigens associated with bovine leukemia virus infection.

We isolated an ether-resistant internal antigen and an ether-sensitive antigen previously described in relation to bovine leukemia virus infection. These two antigens have now been isolated by isoelectric focusing and concanavalin A affinity chromatography, respectively. The ether-resistant antigen exhibited isoelectric heterogeneity with a major peak at pH 7.2 and a minor peak at pH 6.2. Its molecular weight, estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), was 23,000 (p23), and it gave a sedimentation value of 2.3s. For material containing ether-sensitive antigen, analyzed by SDS-PAGE, protein staining revealed four components with molecular weights of 18,000, 25,000, 45,000, and 55,000. Two of these [45,000 (gp45) and 55,000 (gp55)] were stained by periodic acid-Schiff reagent. Isoelectric point and sedimentation value of the major glycoprotein (gp45) were pH 5.0 and 3.4s, respectively; no immunologic cross-reactivity was found between p23 and glycoprotein antigen.

Animals↗

PubMed1976

Subtypes of hepatitis B surface antigen in native-born and immigrant Israelis.

A survey of HBsAg among Jerusalem blood donors revealed a prevalence of 0.89% and a predominance of subtype ay (85%) compared with ad (15%). A simultaneous survey of patients with HBsAg-positive viral hepatitis revealed a similar predominance of ay (85%) compared with ad (15%). The uniform distribution of the dominant ay subtype among both carriers and patients, representing a diversity of ethnic and national origins, supports the premise that ad and ay subtypes are preferentially correlated with regional epidermiologic, rather than host or disease-related factors.

Blood Donors↗

PubMed1976

Quantitation of bovine papilloma viral DNA in viral-induced tumors.

Bovine papilloma virus (BPV) DNA was labeled in vitro under conditions of repair synthesis and subsequently used as a "probe" in DNA-DNA reassociation studies to detect BPV-specific DNA sequences in a viral-induced calf meningioma and hamster fibroma. In vitro labeled BPV DNA had denaturation characteristics expected for duplex DNA and denatured DNA reassociated with apparent second-order kinetics. Analysis of in vitro labeled BPV DNA reassociation rates in the presence of excess tumor DNA revealed that the calf meningioma contained approximately 700 to 800 BPV genome equivalents per diploid cell whereas the hamster fibroma contained about 150 incomplete BPV genome equivalents per diploid cell. Thermal denaturation of in vitro labeled BPV DNA which reassociated in the presence of the two tumor DNA preparations indicated less than 1.5% base pair mismatching.

Animals↗

PubMed1976

Precipitin response of cattle to commercial wart vaccine.

Fifty young bulls were given commercial wart vaccine upon their arrival at a bull stud establishment. The bulls were given 2 additional vaccinations 2 and 24 weeks later. The precipitin antibody response of these bulls to bovine papilloma virus was monitored. Antibody levels increased significantly, particularly after the 3rd vaccination was given at 24 weeks, but decreased markedly within 20 weeks after the last vaccination was given. These results demonstrated that formalin-inactivated bovine papilloma virus was capable of inducing precipitin antibody similar to infective bovine papilloma virus. Multiple, repeated vaccination provided the best serologic response.

Animals↗

PubMed1976

Pathology of lymphosarcoma in sheep induced with bovine leukemia virus.

Sixty-nine sheep were infected with bovine leukemia virus from bovine lymphosarcoma materials. Twenty-four developed lymphosarcoma and died from 13 to 66 (average, 29) months later. Circulating lymphocytes were increased to leukemia levels (70,000 to 403,000/cu mm blood) in only eight sheep within 2 to 3 months of death. Various lymph nodes and visceral organs including heart, abomasum, uterus, kidneys, and urinary tract were commonly affected as in cattle with the adult form of lymphosarcoma. In one sheep the skin was involved. The liver was involved in only one case. This was in contrast to more frequent involvement reported in literature for naturally occurring lymphosarcoma. The neoplasms in experimental sheep are regarded as a mixture of reticulum or histiocytic cells and lymphoid cells with transitional forms supported by a usually sparse and diffuse fibroplasia and a web of silver-staining reticulin fibers.

Animals↗

PubMed1975

Survey for antibodies to leukemia (C-type) virus in cattle.

Serums from 4,394 dairy cattle in 100 herds and from 2,794 beef cattle in 50 herds were tested for antibody to the bovine (C-type) leukemia virus (BLV), using the agar gel immunodiffusion test. Reactors were found in 66% of the dairy herds (10.2% of the cattle) and in 14% of the beef herds (1.2% of the cattle). The prevalence of reactors was examined with respect to age, herd size, and sex. Few of the reactors were less than 2 years old. There was a high percentage of reactors in small dairy herds (less than 50 cattle). In 22 dairy herds (1,354 cows and 96 bulls), the rate of infection in cows was compared with that in bulls. In those herds, 13.5% of the cows and 10.4% of the bulls were reactors.

Age Factors↗

PubMed1975

Inhibition of bovine leukemia virus release.

Sera from 3 cows with the adult form of lymphosarcoma inhibited release of leukemia virus from a cell line of fetal lamb spleen infected with bovine leukemia virus (BLV). Sera from 5 to 7 cattle experimentally infected with BLV also suppressed virus release. The inhibition of virus release was reversible. Sera from cattle with the calf form and the thymic form of lymphosarcoma and normal bovine control sera did not repress virus release.

Animals↗

PubMed1975

An ether-sensitive antigen associated with bovine leukemia virus infection.

An ether-sensitive antigen (es-Ag) associated with bovine leukemia virus infection was detected by immunodiffusion tests. This antigen was sensitive to ether, sodium periodate, and trypsin treatment. Based on column chromatography, es-Ag was a larger molecule than that of ether-resistant antigen (gs-Ag). With infection, precipitating antibody against es-Ag developed earlier than the precipitating antibody against the gs-Ag.

Animals↗

PubMed1975