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Biomedical subjects

C P Crum

Publications and source records attributed to C P Crum.

At least 145 records · Page 8Linked to original sources
PubMed1983

Atypical immature metaplasia (AIM). A subset of human papilloma virus infection of the cervix.

Atypical immature squamous metaplasia (AIM) is a poorly understood lesion with uncertain biological and clinical significance. This report reviews 170 cases of cervical condylomata and 60 cases of high-grade cervical intraepithelial neoplasia (CIN II/III); AIM was found in association with 34% of condylomata and 16% of high-grade CIN. Thirty-seven cases of AIM alone were reviewed and nearly all presented with a cytologic diagnosis of CIN I or condyloma and a colposcopic appearance of white epithelium with or without punctation and mosaic structure. The mean ages of patients with condylomata, AIM, CIN II, and CIN III were 27, 27, 32, and 37 years, respectively. By immunoperoxidase techniques 75% of condylomata, 16% of AIM, and 0% of CIN lesions were positive. The histologic criteria for the diagnosis of AIM and it's morphologic distinction from CIN and relationship to condylomata, are outlined. AIM is a distinct histologic entity that shares similar epidemiologic, morphologic and biologic characteristics with condyloma. When AIM is found alone in biopsy material, careful correlation of cytology, and biopsy results should be performed and therapy should be based on the size and distribution of the lesion.

Adult↗
PubMed1983

Human papilloma virus infection (condyloma) of the cervix and cervical intraepithelial neoplasia: a histopathologic and statistical analysis.

Two hundred fifty consecutive cases previously diagnosed as advanced intraepithelial neoplasia (CIN 2 and CIN III) were reviewed and examined for the presence of coexisting condyloma. When the mean ages and age distributions were analyzed, there was a consistent shift of the age distribution to a younger age among women with CIN and condyloma as opposed to CIN alone. Furthermore, when the relationship between age and case accumulation was normalized from a curvilinear to a linear relationship, there were significant differences in the slope of the accumulation rate between cohorts of CIN III with and without condyloma. These differences suggest that the concordant observation of both lesions in a given patient is not a random process resulting from two independent events but rather indicates an underlying dependence between the two (i.e., condyloma and CIN). It cannot, however, be determined statistically whether the associated condyloma is directly responsible for the CIN lesion or hastens its transit time.

Adult↗
PubMed1983

Cervical papillomavirus infection in diethylstilbestrol-exposed progeny.

Histologic sections from cervical and vaginal biopsies showing dysplasia in 37 women exposed to diethylstilbestrol (DES) in utero were stained for human papillomavirus structural antigens using an immunoperoxidase technique. Forty-three percent of the lesions had detectable papillomavirus antigens. These findings indicate that a significant proportion of cervical dysplasia observed in DES-exposed progeny is associated with papillomavirus infection.

Animals↗
PubMed1982

Intraepithelial squamous lesions of the vulva: biologic and histologic criteria for the distinction of condylomas from vulvar intraepithelial neoplasia.

We reviewed 65 intraepithelial lesions of the vulva and distal vagina and compared the presence of koilocytosis, abnormal mitoses, and parabasal or basal nuclear enlargement with DNA microspectrophotometric distribution patterns and the presence of human papillomavirus antigen as determined by immunoperoxidase. Abnormal mitoses and cytologically atypical nuclear enlargement were specific predictors of aneuploidy and were reliable for distinguishing vulvar intraepithelial neoplasia (VIN) from condylomas. Koilocytosis was present in 100% of condylomas and 71% of aneuploid (VIN) lesions, but there were qualitative and quantitative differences in the distribution of koilocytic cells in the two classes of lesions. On the basis of these findings, criteria for distinguishing between VIN and condyloma are proposed.

Antigens, Viral↗
PubMed1982

Treatment of vaginal carcinoma in situ with the carbon dioxide laser.

Thirty-six patients with vaginal intraepithelial neoplasia were treated with the carbon dioxide laser. More than half of the patients had grade 3 vaginal intraepithelial neoplasia (carcinoma in situ). The majority of patients were treated in a single session; six patients were treated twice, and two patients were treated three times. In 92% of the patients, the lesions were completely removed by the laser without significant side effects.

Adult↗
PubMed1982

Vulvar intraepithelial neoplasia: correlation of nuclear DNA content and the presence of a human papilloma virus (HPV) structural antigen.

Immunoperoxidase localization of a human papilloma virus structural antigen (HPV) was attempted in 68 intraepithelial lesions of the vulva, 39 of which were analyzed for nuclear DNA content by microspectrophotometry. Overall, 5.9% (4/68) stained positive for HPV. Ninety percent (35/39) of the cases tested were aneuploid, and, of these, 2.8% (1/35) stained positive for HPV. In contrast, 50% (2/4) of the polyploid lesions were positive. Hence DNA microspectrophotometry and immunoperoxidase localization of HPV are useful coparameters for distinguishing wart virus infection (condylomata) from vulvar intraepithelial neoplasia. HPV is detected infrequently within aneuploid lesions, in keeping with the concept that epithelial maturation is required for virion assembly. Whether the HPV genome exists in a nonreplicative state within the aneuploid cell population is unknown.

Antigens, Viral↗
PubMed1982

Endometrial hyperplasia and carcinoma. Are ultrastructural, biochemical and immunocytochemical studies useful in distinguishing between them?

Endometrial hyperplasia and carcinoma represent different points in a disease continuum which may be difficult to distinguish using standard histologic criteria. The role of ancillary biochemical, ultrastructural and immunocytochemical techniques in understanding the normal physiologic responses to hormonal stimuli is briefly considered in order to serve as a basis for an analysis of abnormal proliferative states. Not surprisingly, the ultrastructural and biochemical features of hyperplastic and carcinomatous endometria demonstrate a progressive continuum of abnormalities. The role of the immunohistologic detection of CEA, HCG, Casein, and other markers in distinguishing between the various endometrial diseases is discussed. The endometrium represents one of the more spectacular of endocrine target organs in which marked morphologic and ultrastructural changes occur throughout a women's lifetime. Such changes include those accompanying the menarche and the menopause as well as the extensive remodelling which occurs during the reproductive years. During the latter period, the endometrium cyclically proliferates, undergoes secretory differentiation, regresses, degenerates and regenerates. These regularly occurring changes are distinctive and have been described in histological detail, (Noyes et al., 1950, Noyes, 1973, Dallenbach-Hellweg, 1974), as well as ultrastructurally (Cavazos and Lucas, 1973, Ferenczy and Richart, 1974, Ferenczy, 1977). Morphological studies, coupled with advances in knowledge of steroid biochemistry, have confirmed that the cyclic morphological alterations are under the regulatory role of estrogens and progesterone.

Adenocarcinoma↗
PubMed1982

Herpesvirus-specific RNA and protein in carcinoma of the uterine cervix.

Cloned probes of herpes simplex virus type 2 DNA were used in cytological hybridization experiments to detect herpesvirus RNA transcripts in the neoplastic cells of tumors of the uterine cervix. Virus-specific RNA was shown to represent transcription of limited regions of the genome, of which one is known to code for a DNA-binding protein that can be found by immunoperoxidase staining in the neoplastic cells of these tumors and has also been detected in cells transformed in vitro by this virus.

Antigens, Viral↗
PubMed1982

Management of vulvar intraepithelial neoplasia by carbon dioxide laser.

Thirty-three patients with vulvar intraepithelial neoplasia were treated with the carbon dioxide (CO2) laser. In 31 of these patients the lesion was completely eradicated by the laser treatment. All patients experienced moderate pain following the procedure, and 2 had severe pain. The only other complications were 1 case of bleeding and 1 of infection. The CO2 laser appears to be an acceptable treatment modality for vulvar cancer precursors provided patients are carefully evaluated prior to treatment and invasive carcinoma is ruled out.

Adult↗
PubMed1982

The expression of human chorionic gonadotropin in the female genital tract: localization by the immunoperoxidase technique.

Four hundred eighty-eight lesions from 456 patients were surveyed for the production of hCG using the immunoperoxidase technique. HCG immunoreactivity was found in 9.1% of the total number of cases studied. The 10% of ovarian cancers expressing hCG included serous, endometrioid, and germ cell tumors. Eighteen point nine percent of neoplastic cervices were positive for hCG; the highest rate of expression was in invasive cancers. Eighteen point nine percent of endometrial cancers were hCG positive; poorly differentiated tumors had the highest percent of marker expression. The significance of "ectopic" hCG expression in nontrophoblastic gynecologic neoplasms is discussed.

Carcinoma, Squamous Cell↗
PubMed1981

Cryptococcal peritonitis complicating a ventriculoperitoneal shunt in unsuspected cryptococcal meningitis.

A 34 year old male was hospitalized because of the probability of a posterior fossa lesion that had increased intracranial pressure. A ventriculoperitoneal shunt was implanted, resulting in partial resolution of symptoms. Subsequently Cryptococcus neoformans was cultured from the cerebrospinal fluid and a diagnosis of cryptococcal meningitis was made. Despite amphotericin B therapy, the patient continued to deteriorate and died on the eighty-fifth day of hospitalization. Autopsy demonstrated cryptococcal meningitis and cerebral edema. An unexpected finding was cryptococcal peritonitis, which was not associated with disseminated disease. The case is unique because cryptococcal peritonitis is rare, and the spread of the organism occurred through a ventriculoperitoneal shunt.

Adult↗