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C P van Beveren

Publications and source records attributed to C P van Beveren.

7 recordsLinked to original sources
PubMed1981

The nucleotide sequence of the gene for protein IVa2 and of the 5' leader segment of the major late mRNAs of adenovirus type 5.

We present here the primary structure of the region of human adenovirus 5 (Ad5) DNA from nucleotide 4001 through the HindIII site at nucleotide 6246 (map positions 0.11 to 0.17). The corresponding region in the closely related adenovirus type 2 (Ad2) encodes the spliced mRNA for viral protein IVa2 (Chow et al., 1979; Persson et al., 1979). Reverse transcription of the Ad5 pIVa2 mRNA localized the 5' terminus of the mRNA to approximately position 5840, and its splice coordinates to positions 5706 and 5427. From the data of Aleström et al. (1980) for Ad2, the 3' end of this mRNA was inferred to be specified by Ad5 nucleotide 4060. The nucleic acid data allow us to predict an Mr of 50873 for the IVa2 protein of Ad5, which is close to the experimentally determined value of 50000 (Persson et al., 1979). The DNA sequence described here also includes the information for the 5'-terminal leader segment of the major late mRNAs of Ad5.

Adenoviruses, Human↗
PubMed1981

Detection and elimination of cellular nucleic acids in biologicals produced on continuous cell lines.

Experiments have been conducted to determine the extent to which currently available purification techniques can remove contaminating substrate cellular DNA from inactivated poliovirus vaccine produced on continuous cell lines rising highly [32P]-labeled, nick-translated cellular DNA added to poliovirus suspensions, we found that purification procedures were capable, in small-scale experiments, of reducing contaminating DNA by factors of 10(3) (DNAse treatment followed by gel filtration) and 10(3)-3X10(5) (ion exchange chromatography). Sequential application of these purification steps should reduce contaminating cellular DNA to acceptable levels. We also examined the potential usefulness of immobilized nucleic acid hybridization techniques for the routine direct testing of residual cellular nucleic acids in final production lots of inactivated poliovirus vaccine and other biologicals. A filter hybridization test, using [32P]-labeled, nick-translated cellular DNA as a probe, was capable of detecting 40 pg of homologous cellular DNA. Using probes of higher specific activity the assay should be sensitive enough for use in routine quality control.

Animals↗
PubMed1980

The nucleotide sequence of the transforming early region E1 of adenovirus type 5 DNA.

The sequence of the leftmost 11.3% of the non-oncogenic human adenovirus type 5 (Ad5) DNA has been determined. This segment contains the entire early region E1 of the Ad5 genome which has been shown to be involved in in vitro transformation of non-permissive rodent cells (Van der Eb et al., 1980). From the DNA sequence, and from the mRNA sequence data obtained by Perricaudet et al, (1979, 1980) for the E1 mRNAs from the closely related adenovirus type 2 (Ad2), it is possible to predict the primary structure of the polypeptides encoded by this region. The function of these proteins in cell transformation is discussed. From the positions of mapped restriction endonuclease sites and termini of RNA segments in the nucleotide sequence the length of the Ad5 DNA is estimated to be 36.6 kb.

Adenoviruses, Human↗
PubMed1980

The nucleotide sequence of adenovirus type 5 early region E1: the region between map positions 8.0 (HindIII site) and 11.8 (SmaI site).

The nucleotide sequence of the region between map positions 8.0 (HindIII site) and 11.8 (SmaI site) of adenovirus type 5 (Ad5) has been determined. Together with the sequences reported earlier (Van Ormondt et al., 1978; Maat and Van Ormondt, 1979) it encompasses the entire leftmost early region E1 of Ad5 DNA (4126 base pairs). The total sequence revealed a number of potential regulatory signals (promoter sites, ribosome binding sites, 3'-poly(A)-associated sequences), which confirm that region E1 is divided into subregions, E1a and E1b, and a region coding for semi-late viral protein IX. By taking into account the adenovirus 2 (Ad2) RNA-splicing data of Perricaudet et al. (1979; 1980) and the Ad2 RNA mapping data of Chow et al. (1979) we predict that E1a codes for polypeptides of 32, 26 and ca. 13 kd, and subregion E1b for polypeptides of 67 kd and 20 kd; the expected molecular weight of protein IX is 14.4 kd.

Adenoviruses, Human↗