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C Pérez-Rico

Publications and source records attributed to C Pérez-Rico.

4 recordsLinked to original sources
PubMed1985

Choline acetyltransferase depletion in the rat retina after intraocular injection of neurotoxins.

The effects of kainic acid (KA), quisqualic acid (QA), and ibotenic acid (IBO) on histology of the retina and on the retinal choline acetyltransferase (ChAT) activity were studied in the rat. KA produced the highest number of altered cells in the ganglion cell layer (GCL) and in the inner nuclear layer (INL), with an almost complete depletion of ChAT activity. QA was less effective than KA in terms of both the number of altered cells and in ChAT depletion. In contrast, retinas injected with IBO showed the mildest morphological lesions together with the highest reduction in the enzyme activity. These results indicate that IBO affects nearly all the cholinergic neurons in the rat retina, whereas other populations, sensitive to KA or QA, are spared. Because of this higher specificity toward the cholinergic subpopulation, IBO may be a useful tool when cholinergic cells need to be destroyed in the retina.

Animals↗
PubMed1983

Morphological alterations of retinal cells accompanying inhibition of axonal transport by kainic acid.

The effect of kainic acid (KA) on rat retinal morphology was investigated with emphasis on the ganglion cell layer. Two hours after intraocular injection of KA, 70% of the neurones in the ganglion cell layer display morphological alterations. These alterations at the ultrastructural level include the absence of identifiable Golgi complexes and the presence of abundant round empty vacuoles among other nuclear and cytoplasmic modifications. These alterations of the Golgi complex are discussed in relation to axoplasmic transport inhibition by kainic acid. Seven days after KA treatment the retina showed a decreased number of cells in the inner nuclear layer and ganglion cell layer and the inner plexiform layer had virtually disappeared. Despite the disappearance of cells from the ganglion cell layer a week after KA injection, abundant ganglion cells could be retrogradely labelled with horseradish peroxidase. Thus although retinal ganglion cells seem to be sensitive to KA most of them survive KA treatment.

Animals↗