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C Padilla

Publications and source records attributed to C Padilla.

24 records · Page 2Linked to original sources
PubMedDate not supplied

Recombinant antigens for specific and sensitive serodiagnosis of Latin American tegumentary leishmaniasis.

The diagnostic potential of recombinant leishmanial antigens for Latin American tegumentary leishmaniasis (LATL) was examined. Two Leishmania (Viannia) peruviana recombinant proteins, T26-U2 and T26-U4, were assessed by their reactivity to detect specific anti-leishmanial antibodies. Seventy-eight individual sera from persons with LATL, 39 from those with other diseases, and 10 negative control sera were tested by Western blotting and enzyme-linked immunosorbent assay. The sensitivity of the test using T26-U2 plus T26-U4 was similar to that obtained with whole parasite extract (92%). However, the specificity obtained using both recombinant antigens (87%) was higher than that of the whole parasite extract (65%). All tests using recombinant proteins (T26-U2, T26-U2 plus T26-U4 or T26-U4) had a higher positive predictive value (89%, 92% and 98%, respectively) than the value obtained using total parasites (81%). Eleven Colombian sera were also tested, and the results indicated that T26-U2 plus T26-U4 could be used successfully in Peru and in other Latin American countries.

Animals↗
PubMedDate not supplied

Acidic ribosomal proteins and histone H3 from Leishmania present a high rate of divergence.

Another additional peculiarity in Leishmania will be discussed about of the amino acid divergence rate of three structural proteins: acidic ribosomal P1 and P2b proteins, and histone H3 by using multiple sequence alignment and dendrograms. These structural proteins present a high rate of divergence regarding to their homologous protein in Trypanosoma cruzi. At this regard, L. (V.) peruviana P1 and T. cruzi P1 showed 57.4% of divergence rate. Likewise, L. (V.) braziliensis histone H3 and acidic ribosomal P2 protein exhibited 31.8% and 41.7% respectively of rate of divergence in comparison with their homologous in T. cruzi.

Amino Acids↗
PubMedDate not supplied

Effects of minimum inhibitory concentrations of three antimicrobials on the growth cell and fimbriation of uropathogenic Escherichia coli.

Uropathogenic E. coli 36692 cells synthesize fimbriae during the first half of the exponential growth curve. The presence of the appendices was detected by electron microscopy and it was correlated with a high haemagglutination activity. Norfloxacin, amoxicillin and gentamicin supply 1/4 of their respective MICs, inhibiting the cell growth. Also all these drugs with the same sub MICs alter the haemagglutination titre and inhibit the fimbriation process of the uropathogenic E. coli. Gentamicin showed the highest inhibition of the haemagglutinating activity and fimbriae synthesis.

Adhesins, Escherichia coli↗