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C Pardo

Publications and source records attributed to C Pardo.

26 records · Page 2Linked to original sources
PubMed1991

On the role of trehalose in yeast cells subjected to hypersomotic shock.

Plate counts on both high and low water activity (aw) media were performed during the growth of Candida utilis in batch culture. The results revealed a marked discrepancy between the counts on the two media in the logarithmic phase. The discrepancy almost disappeared in stationary phase revealing a higher resistance of these cells, as compared to growing cells, to the severe dehydration imposed by the hyperosmotic shock upon transfer to low aw medium. Since the two types of cells differ in the level of endogenous trehalose the relation between plating discrepancy and trehalose content of the cells was investigated. Treatments that changed the intracellular trehalose concentration did not modify the plate counts on low aw medium. It was therefore concluded that the amount of trehalose into the cells is not the only factor conferring resistance against the hyperosmotic shock. Glycerol content did not correlate with resistance to the water stress either. Congruent with the former conclusion, other yeast species (Sporobolomyces salmonicolor, Schizosaccharomyces pombe) showed no correlation between changes in the trehalose content and susceptibility or resistance to the osmotic stress in low aw medium.

Candida↗
PubMed1989

[Course of diabetic retinopathy and metabolic control with subcutaneous insulin infusion pump: 18-month study].

The effect of a good metabolic control in the natural history of diabetic retinopathy is discussed because, although the onset and the incidence are closely related to a bad metabolic control, there is no improvement related to a good control; progression having been reported with the good metabolic controls. 7 patients subjected to a strict control with continuous subcutaneous insulin infusion were compared with a group of 10 patients with a good conventional treatment in an 18 month study. The best metabolic control of the first group was obtained at 9, 12, 15 and 18 months (p less than 0,05). The evolution of retinopathy in this group did not change with the treatment and there was no proof of progression with the use of continuous subcutaneous infusion. Further follow-up evaluation is necessary to confirm this affirmation.

Diabetes Mellitus, Type 1↗
PubMedDate not supplied

Comparison of two low-calorie diets: a prospective study of effectiveness and safety.

OBJECTIVE: To evaluate the cost-effectiveness and safety of two distinct low calorie diets (LCD). DESIGN: Prospective controlled study. METHODS: 67 obese patients [body mass index (BMI) 40 kg/m2] were included in two study groups. Group A: 26 patients followed a 458 kcal diet given in three meals for 1 month. Group B: 41 patients followed a 800 kcal diet for 3 months and with outpatient control. MEASUREMENTS: Anthropometric, cardiovascular risk and nutritional profile changes were evaluated, as well as total direct and indirect costs, and the incidence of complications. RESULTS: No significant initial differences were observed between the two study groups. Eighty-six point two per cent of the patients completed the therapy correctly. After treatment a significant decrease was observed in the following variables for both groups, but no differences were detected between Groups A and B: mean weight loss (A= 9.28 kg, B= 8.7 kg), ponderal loss percentage (A/B= 7.2/6.8%), glycemia (A/B= 18.6/12.1 mg/dl), systolic blood pressure (SBP) (A/B= 11.8/6.5 mmHg), diastolic blood pressure (DBP) (A/B 5.9/6.8 mmHg), and final insulin-resistance (IR) index (A= 4.4, B= 4.3). Group A had the highest drop in total cholesterol (37.7 vs 8.1 mg/dl) and triglycerides (54.4 vs 2.5 mg/dl). No changes were observed in ureic acid, renal function and serum albumin. Thirty-six patients (55.3%) suffered trivial complications associated to the VLCD (16.9% gastrointestinal, 20% anxiety), with no differences between groups. Group A patients were on sick leave due to asthenia, and two patients in this group had serious complications (transient ischemic attack and atrial fibrillation). The total cost of Group A treatment was 3018.9 against 582.6 euros for Group B. CONCLUSIONS: The 3-month 800 kcal/day VLCD was more cost-effective and safer than the 1-month 458 kcal/day diet.

Adult↗
PubMedDate not supplied

[CD44s Cell surface expression in 55 lung adenocarcinomas. Negative correlation with tumor size and CA125 cytosolic levels].

INTRODUCTION: CD44s belongs to a family of cell adhesion molecules involved in cell adhesion, migration and cell-extracellular matrix interactions. In this work we attempt to study CD44s expression in lung adenocarcinomas and its possible correlation with other clinicobiological parameters. MATERIAL AND METHODS: Using an EIA, cell surface CD44s levels were determined in 55 lung adenocarcinomas, classified according to clinical stage, histological grade, ploidy and cellular S-phase fraction. CA125 cytosolic concentrations were also assayed. RESULTS: Forty two adenocarcinomas (76.4%) showed CD44s concentrations > 80 ng/mg prot, and did not differ significantly from those observed in 16 normal samples (93.7%). There were no differences in CD44s expression when clinical stage (I: 24/28, II: 6/9 and III: 12/17), lymph node involvement (N = 245/29, N+: 18/26), ploidy (diploid: 3/5, aneuploid: 32/39), histological grade (I: 6/7, III: 18/26) and cellular S-phase (> 8.8%: 24/31, < or = 8.8%: 17/24) were considered. Positive CD44s tumors had lower CA125 (p: 0.0072) cytosolic levels and a reduced tumor size (p: 0.0093). CONCLUSIONS: CD44s expressions in lung adenocarcinomas did not correlate with any clinicobiological parameters, but there was a negative correlation between this and reduced tumor size and lower CA125 cytosolic levels.

Adenocarcinoma↗
PubMedDate not supplied

[Jugular phlebectasia. Apropos a case].

We present a case of 6-years-old male with an internal jugular phlebectasia, diagnosed at our Hospital. Review of the bibliography, and complementary examination, like doppler, echography and digital intravenous angiography subtraction (DIVAS) was made.

Angiography, Digital Subtraction↗