PubMed Health⌕ Search

Biomedical subjects

C Patel

Publications and source records attributed to C Patel.

At least 91 records · Page 5Linked to original sources
PubMed1983

Nucleoli, micronucleoli, and nucleolus-like structures in human oocytes at meiotic prophase I studied by the silver-NOR technique.

Meiotic nuclei preparations obtained from human fetal ovaries were studied with the silver-NOR technique. At leptotene, the NOR's were located at the periphery of the nucleoli. The mean number of NOR's per nucleus was 6.4. Moreover, 2-12 micronucleoli, each containing an Ag-positive zone, were observed. At pachytene, each nucleolus was divided into two zones, one of which was Ag-positive. The mean number of Ag-positive zones was 2.1 per nucleus. The subterminal region of the short arm of nucleolar bivalents was in contact with the argyrophilic nucleolar zone. In most cells, micronucleoli were dispersed between the bivalents. A single rounded body which stained heavily with silver was consistently observed. At early diplotene, the NOR's separated, appearing again as distinct rounded Ag-positive structures embedded in the peripheral part of the nucleoli. The silver-stained round body was still visible. Eight to twelve micronucleoli were observed. At a more advanced stage of diplotene, the number of micronucleoli increased, varying from 20 to 50 per nucleus. These observations suggest that the micronucleoli, already visible at the leptotene stage, are the morphological expression of an early transcription of amplified rDNA. The biological significance of the Ag-positive round body remains obscure, but the consistency of its appearance suggests that it has a specific function somehow related to nucleolar proteins.

Cell Nucleolus↗
PubMed1982

Augmentation of flap survival by thymoxamine.

An experimental study in rats showed that the use of the alpha-receptor antagonist, thymoxamine could increase the blood supply to an ischaemic flap. Thymoxamine has a very short half-life, must be given frequently to be effective, but has the significant advantage that its action can be reversed by noradrenaline. When a flap is in trouble, alpha-receptor blockade by thymoxamine might help dramatically in its salvage.

Animals↗
PubMed1982

Improved stability of methanolic Wright's stain solution with dual additives.

Degradation of methanolic Wright's stain solutions was greatly diminished with the addition of diethylamine hydrochloride and dimethylamine hydrochloride as costabilizers. Precipitation problems were eliminated by the dual additives. The stabilized stain solutions demonstrated good staining performance on blood smears. Methods for predicting the shelf life using calculated analytical parameters are described. Using these methods, the shelf life of a control stain solution was predicted to be 0.7 years; predicted shelf life was more than tripled with the addition of diethylamine hydrochloride and was increased approximately 27 times with the addition of both diethylamine hydrochloride and dimethylamine hydrochloride.

Blood Cells↗
PubMed1981

Controlled trial of biofeedback-aided behavioural methods in reducing mild hypertension.

Employees of a large industry were screened for the presence of coronary risk factors. A total of 204 employees, aged 35-64 years, with two or more such factors (serum cholesterol concentration greater than or equal to 6.3 mmol/l (243.6 mg/100 ml), blood pressure greater than or equal to 140/90 mm Hg, and current cigarette consumption greater than or equal to 10 cigarettes a day) were randomly allocated to a biofeedback group receiving training in relaxation and management of stress or a control group. Both groups received simple health education literature. After eight weeks of training, and again eight months later, the biofeedback group showed a significantly greater fall in systolic and diastolic blood pressures than the control group (p less than 0.001). Plasma renin activity and plasma aldosterone concentration were measured in a subsample at entry to the study and again at eight weeks and eight months; both showed a greater reduction in the biofeedback compared with the control group at eight weeks' follow-up. The greater reduction in blood pressure in the subjects in the biofeedback group compared with the control group (11.0 mm Hg systolic and 8.8 mm Hg diastolic), persisting eight months after the training, suggests that relaxation-based behavioural methods might be offered as a first-time treatment to patients with mild hypertension.

Adult↗
PubMed1981

Improved stability of methanolic wright's stain with additive reagents.

Additive reagents have been investigated to improve the stability of methanolic Wright's stain. The addition of ammonium halides, monoalkylamine hydrochlorides, dialkylamine hydrochlorides or trialkylamine hydrochlorides to methanolic Wright's stain was found to enhance the stability of stain components in methanol. No change in performance is observed with these additives present. Random precipitation in the stain solution was still observed with the addition of ammonium halides and monoalkylamine hydrochlorides. No precipitation was found in stain solutions containing hydrochlorides of most dialkylamines and trialkylamines. Of the compounds evaluated, 0.6% diethylamine hydrochloride added to methanolic stain solutions produced the most desirable overall results. Mechanisms of stabilization and precipitation in these stain solutions are proposed. Essentially, separation of the thiazine-eosinate ion pair through interaction with an appropriate additive increases stain stability. The solubilities of thiazine-eosinate or additive cation-eosinate ion pairs in methanol determine the formation of precipitate in such stain solutions.

Amines↗
PubMed1981

Routine cytodiagnosis of pulmonary malignancies.

Anesthesiologists normally discard the material routinely suctioned from the tracheal tube of patients under general endotracheal anesthesia. We preserved, and later examined microscopically, specimens from 10,621 patients. We found 11 cytologically abnormal smears from subjects with unsuspected pulmonary involvement, an incidence slightly more than 1:1,000. The accuracy of our method was assessed by calculation of the percent of abnormal smears obtained from patients with prediagnosed bronchogenic carcinomas: 40% when suctioned material was immediately spread on slides, and 67% when cellular concentration was achieved by mucolysis followed by filtration or centrifugation. Secretions normally discarded can reveal much information that would otherwise be missed. Our method may be useful if applied to patients at risk (heavy smokers, workers with asbestos, nitrosamine, or benzopyrine, miners of radioactive material, etc) who undergo general anesthesia for incidental surgery.

Adolescent↗
PubMed1980

Effects of minute volume increases on the rebreathing characteristics of the Bain anaesthesia circuit during controlled ventilation.

In the course of a study on the carbon dioxide rebreathing characteristics of the Bain anaesthesia circuit, it was noted that raising the minute volume without changing the fresh gas inflow invariably led to increased rebreathing of expired gases. Altering tidal volume and rate in order to reproduce a given minute volume had the same effect on rebreathing. A nomogram constructed to quantitate increases in rebreathing in function of carbon dioxide production per minute, fresh gas flow from the anaesthesia machine, and minute volume is produced. It can be used to assess the amount of fresh gas flow necessary to mantain a steady and inspired gas composition.

Anesthesiology↗
PubMed1980

A screening test for influenza immunity: preimmunization antibody titers to influenza virus antigens in atopic patients.

Antibody titers to three common strains of influenza virus, including the Swine Flu strain, were determined in 152 patients in an allergy and pediatric allergy practice prior to planned immunization with influenza vaccine. Because allergic patients are considered to be more prone to hypersensitivity reactions than individuals who are non-allergic, it was deemed prudent to screen for patients who might have pre-existing protective levels of antibody to the viruses. A considerable number of the 152 patients were found to have titers of 1:20 or 1:40 to one or more of the three influenza strains, including the Swine Flu strain. The largest percentage of positive antibody levels was found to be to the A-Victoria strain which has recently been most prevalent in the northeastern United States. The lowest numbers of patients with protective antibody levels were for the Swine Flu strain. Nevertheless, the highest titers occurred with patient specimens tested against the Swine Flu strain. Approximately one-quarter of the patients showed significant levels of antibody (1:40 or more) to one or more of the virus antigens and thus were not vaccinated, avoiding the possibility of untoward areactions which habe been observed in some individuals who have been given the vaccine.

Adolescent↗
PubMed1979

Investigation of reaction intermediates of the urea-diacetylmonoxime reaction.

1. An investigation of the intermediates of the urea-diacetylmonoxime reaction and the isolation of the protochromogen has been described. A comparative spectral study of the protochromogen with that of the literature values suggested the structure of the protochromogen to be a diureide. 2. The diureides of 1-phenyl-1,2-propanedione-monoxime and 2,3-pentanedione were synthesized and their visible, U.V., I.R., NMR, and mass desorption spectrometry data were analyzed. The study suggests that the structure of each of these compound is that of a diureide. When these diureides were redissolved in acid media, they gave the color expected of the corresponding chromogens found from the respective diketones and urea. 3. The 3a-methyl-6a-phenyl-glycoluril was synthesized by a base-catalyzed reaction. When this glycoluril was dissolved in concentrated HCl, a pink color was obtained, similar to that of the corresponding diureide. These studies indicate that both the diureide and the glycoluril (if formed at all in acid-catalyzed reaction) are converted to the same chromogen, possibly via a rearrangement. 4. When 2,3-butanedionemonoximethiosemicarbazone was reacted with urea in acid medium, characteristic spectral data were obtained which were very similar to those of the urea-diacetylmonoximethiosemicarbazide reaction. This tends to indicate that the possible structures of the protochromogens are similar and of the open chain diureide type.

Acetone↗
PubMed1979

Humidity and the anesthetized patient.

Damage to the ciliated cells of the tracheobronchial tree and incidence of postoperative pulmonary complications were measured by point-scoring systems in 202 patients who breathed dry and humidified anesthetic gases for 225 +/- 78 min. The incidence of postoperative pulmonary complications decreased as the humidity of administered anesthetic gases increased from 0 to 32.5 mg H2O/l. A similar relationship was found between the amount of inhaled moisture and the damage to the ciliated epithelium of the tracheobronchial tree. These results appear to indicate that a high inspired humidity is beneficial for operations on normothermic patients, and that cellular damage caused by dryness is a possible contributory factor in the production of the pulmonary atelectasis that follows stoppage of the mucociliary transport system in the immmediate postoperative period.

Adolescent↗
PubMed1979

Muscular dystrophy in an X; 1 translocation female suggests that Duchenne locus is on X chromosome short arm.

A unique combination of a Duchenne-like muscular dystrophy in a girl with a translocation-inversion rearrangement involving an X chromosome and a no 1 chromosome appeared as a result of both gene mutation and chromosome mutation in the mother. The X-autosome rearrangement would permit full expression of an X-linked recessive gene, such as that for Duchenne muscular dystrophy, in a female, and this would satisfactorily explain the characteristic Duchenne-like course of our patient's illness. The simultaneous de novo appearance of the Duchenne mutation and the X;1 rearrange suggests possible sites for the Duchenne locus on the X chromosome short arm (at Xp1106 or Xp2107).

Child↗
PubMed1978

Disseminated Cryptococcus treated with transfer factor.

Cardiac toxic reactions and pulmonary consolidation in the left lower lobe developed in a patient who was receiving amphotericin B therapy for cryptococcal meningitis. Following surgical resection of the lobe, multiple subcutaneous cryptococcal abscesses appeared. Flucytosine administered intravenously failed to eradicate the lesions. Transfer factor therapy and multiple drainage procedures elimniated the skin abscesses. Transfer factor therapy was administered for one year; the patient was asymptomatic 16 months after therapy was discontinued.

Abscess↗