A general-purpose pulsed field controller.
A pulsed field controller compatible with various types of electrophoresis tanks and power supplies is described. The system is based on a high-precision timing circuit, with an output isolated from the main high voltage supply, and four high voltage transistors. The switching time is programmed manually between 5-160 s. With a transverse alternating field electrophoresis system, intact yeast chromosomes could be completely separated after 24 h.
Chromosomes, Fungal↗