Nursing care study: best at bathtime.
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Biomedical subjects
Publications and source records attributed to C R Curtis.
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Verrucarins A (4) and B (5) and verrucarin A acetate, in vivo antileukemic inactive fungal metabolites, have been oxidized with m-chloroperoxybenzoic acid to give verrucarin beta-9,10-epoxides which show high in vivo activity against P388 mouse leukemia.
An action spectrum was determined for the photoinduced formation of perithecia in a homothallic strain of Nectria haematococca Berk. and Br. var. cucurbitae (Snyder and Hansen) Dingley. Dose-response curves for perithecial formation were obtained from 340 to 510 nanometers at 10-nanometer intervals. Radiation longer than 510 nanometers was not effective for inducing perithecial formation. The action spectrum indicated peaks of activity near 360, 440, and 480 nanometers with shoulders near 420 and 460 nanometers. Minima occurred near 350 nanometers, 390 to 410 nanometers, and 470 nanometers. The general shape of this action spectrum appears to be similar to those obtained for many different near ultraviolet-blue-sensitive organisms in which a flavoprotein molecule was postulated to be the photoreceptor.
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Medical records of 210 horses that survived ventral midline celiotomy for at least 4 months were examined and owners were queried to determine factors contributing to incisional hernia formation. The incidence rate of incisional hernias within 4 months was 16%. Factors significantly associated with occurrence of incisional hernias were incisional drainage, closure of the linea alba with chromic gut suture material, previous midline celiotomy, excessive incisional edema, castrated male sex, postoperative leukopenia, and postoperative pain (colic). Factors not significantly associated with occurrence of incisional hernias were suture pattern used for linea alba closure, concurrent enterotomy or intestinal resection, postoperative bandage or stent, postoperative fever, hypoproteinemia, diarrhea, respiratory disease (coughing), and peritonitis. Hernias developed in horses within 12 weeks of surgery, with the earliest hernia recognized at week 2. Of 30 horses for which information was available, only one hernia developed in 24 (80%) and two or more hernias developed in 6 (20%) along the incision. Multiple hernias tended to be smaller than single hernias.
The accuracy of an immunoglobulin (Ig) G test kit for the semiquantitative measurement of IgG concentration was evaluated with serum from 88 foals. Failure of passive transfer (IgG less than 400 mg/dl) was correctly identified in each of 34 samples, and partial failure of passive transfer (400 less than or equal to IgG less than 800 mg/dl) was correctly identified in each of nine samples. Evidence of adequate passive transfer (IgG greater than or equal to 800 mg/dl) was detected in 44 of 45 samples. One sample with 800 mg/dl or more of IgG was incorrectly classified as a partial failure of passive transfer (Kendall Tau - b = .975). The high degree of accuracy, especially without any errors of overestimation of IgG concentrations, indicated that the IgG test kit should be a useful assay for rapidly determining the passive transfer status of foals.
Splenomegaly confirmed by surgery or necropsy in 100 dogs was diagnosed histologically as benign neoplasia (n = 1), primary splenic malignancy (n = 59), neoplastic metastases (n = 6), and nonneoplastic disease (n = 34). Dogs with known systemic disease, such as lymphoma and mast cell tumor, that caused splenomegaly were not included in the study. Hemangiosarcoma was the most common splenic disease (43 cases). Overall mean age of the dogs was 10.7 years, the most common breed was German Shepherd dog, and 72 of the dogs weighed more than 21 kg. Dogs with anemia, nucleated red blood cells, abnormal red blood cell morphology, or splenic rupture had a significantly greater chance of having splenic neoplasia (P less than 0.002). A multivariable logistic regression analysis found that the presence of anemia and splenic rupture in dogs with splenomegaly was up to 69% accurate in predicting presence of splenic neoplasia. After splenectomy, the median survival time of dogs with splenic neoplasia was 13 weeks. For dogs with nonneoplastic splenomegaly it was at least 36 weeks.
This study was designed to evaluate serum bile acid measurements as indicatory, of liver function and/or hepatic fat infiltration in dairy cattle. Serum bile acid concentrations were measured in healthy dairy cattle at different stages of lactation after fasting or feeding. Bile acid concentrations were compared with liver fat content and sulfobromophthalein (BSP) half-life (T 1/2). Serum bile acid concentrations were higher in cows in early lactation and with higher daily milk production. Compared with prefasting values, bile acid concentrations were decreased at 8, 14, and 24 hours of fasting. Blood samples from fed cows at 1- to 2-hour intervals had wide and inconsistent variations in bile acid concentration. Because serum bile acids correlated well with BSP T 1/2, it is suggested that both measurements evaluate a similar aspect of liver function. Neither bile acids nor BSP T 1/2 correlated with differences in liver fat content among cows. Because of large variability in serum bile acid concentrations in fed cows and the lack of correlation of measured values with liver fat content, bile acid determinations do not appear useful for showing changes in hepatic function in fed cows with subclinical hepatic lipidosis nor serve as a screening test for this condition.