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C Ramacciotti

Publications and source records attributed to C Ramacciotti.

4 recordsLinked to original sources
PubMed2006

[Diffuse uptake of brown fat on computed-tomography coupled positron emission tomoscintigraphy (PET-CT) for the exploration of extra-adrenal pheochromocytoma].

We report an observation of strong bilateral uptake on a PET-CT scan compatible with activation of brown adipose tissue in a patient with extra-adrenal pheochromocytoma. A 42-year-old man was hospitalized for hypersudation together with weight loss and palpitations. Heart rate was 120 bpm and fasting blood glucose 1.36 g/l. Endocrine explorations revealed elevated serum chromogranine which reached 517 ng/ml (19-38). The norepinephrine level reached 49.7 nmol/l (<4.00) and urinary norepinephrine and normetanephrine levels reached 13977 nmol/24h (<414) and 32 micromol/24h (0.4-2.5) respectively. The thoraco-abdominal and pelvic scan showed a 6 cm diameter paraaortic hypervascularized mass with an infiltrative lesion of both perirenal area and mediastinal tissue without adenopathies. The abdominal MRI revealed the mass with a low intensity signal in T1 and a slight high intensity signal in T2. MIBG and octreoscan scintigraphies were negative. 18F-DG PET showed intensed uptake in the tumor mass together with intense, diffuse and bilateral uptake above and below the diaphragm. The mass was resected. Histological examination of the surgical specimen confirmed the diagnosis of extra-adrenal pheochromocytoma with an index of 13% cellular proliferation without cell atypia. There was a hypervascularization with small islets of brown adipose tissue in the perirenal fat. Both plasmatic and urinary catecholamines decreased to the normal range after the operation and PET-scan normalized. Bilateral spread of the radiotracer uptake was probably due to brown adipose tissue activation by excessive sympathetic stimulation induced by catecholamines released by the tumor.

Abdominal Neoplasms↗
PubMed1984

Normoprolactinemic galactorrhea in a fertile woman with a copper intra-uterine device (copper IUD).

We report a case of galactorrhea in a normoprolactinemic fertile woman (30 years old) wearing a copper intra-uterine device (Gravigard). The Gravigard was first inserted in July 1977. In February 1979 our patient noted spontaneous galactorrhea, mainly on the left, but it was also present on the right, after breast pressure. X-ray film of the sella turcica, visual-field examination, thyroid function and basal prolactin levels were all within normal limits. In May 1979 the Gravigard was withdrawn and milk loss stopped finally in December 1979. In March 1980 the IUD was replaced; after only 3 days, mild spontaneous lactation again ensued, on the right side. The patient never took drugs which might have occasioned a prolactin rise. Possible explanations for this unusual phenomenon are discussed.

Adult↗
PubMed1983

Polymorphism of human thyroxine-binding globulin.

T4-binding globulin (TBG) consists of a single polypeptide chain containing 4 oligosaccharide units with an average of 10 terminal sialic acid residues. Isoelectric focusing (IEF) of TBG purified from pooled human plasma showed 4 main bands isoelectric at pH 4.2, 4.3, 4.5, and 4.6. Removal of 85% of carbohydrates by treatment with mixed glycosidases reduced these bands to 2, at pH 5.4 and 5.7. This residual microheterogeneity was not related to protein-ampholyte complexes, since it was still present in 8 M urea. It also did not represent an equilibrium mixture of interchangeable conformations, since each band obtained in the first dimension IEF gave a single spot when rerun in a second dimension. Therefore, the residual microheterogeneity of TBG after removal of carbohydrates can be attributed to variation in amino acid composition. Since genetic polymorphism of TBG was recently demonstrated, we further investigated whether the residual microheterogeneity was genetically determined. Plasma samples from 20 white donors and 17 black donors were labeled with [125I]T4 and submitted to IEF, followed by autoradiography. TBG-1, found in white donors and most black donors, showed the same 4 bands as TBG purified from pooled plasma. Two less frequent phenotypes were found in black individuals: TBG-2, with 4 bands at approximately pH 4.25, 4.45, 4.55, and 4.7; and TBG-1,2, having all of the bands present in TBG-1 and TBG-2. Electrophoretically homogeneous preparations of TBG of each type were obtained from 100 ml plasma; after deglycosylation, TBG-1 revealed 2 bands isoelectric at pH 5.4 and 5.7, TBG-2 had 2 bands at pH 5.7 and 5.9, and TBG-1,2 had 3 bands at pH 5.4, 5.7, and 5.9. The same TBG bands were found after neuraminidase treatment of whole plasma from the same donors. These data demonstrate two kinds of TBG polymorphism. The first is found in deglycosylated TBG from individual donors and is probably due to variation in amino acid composition. The second, also unrelated to the carbohydrate moiety, is a genetic polymorphism found in blacks.

Black People↗