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C Raman

Publications and source records attributed to C Raman.

At least 19 recordsLinked to original sources

Detecting level crossings without looking at the spectrum.

In many physical systems it is important to be aware of the crossings and avoided crossings which occur when eigenvalues of a physical observable are varied using an external parameter. We have discovered a powerful algebraic method of finding such crossings via a mapping to the problem of locating the roots of a polynomial in that parameter. We demonstrate our method on atoms and molecules in a magnetic field, where it has implications in the search for Feshbach resonances. In the atomic case our method allows us to point out a new class of invariants of the Breit-Rabi Hamiltonian of magnetic resonance. In the case of molecules, it enables us to find curve crossings with practically no knowledge of the corresponding Born-Oppenheimer potentials.

Journal Article↗

A model for drug release from fast phase inverting injectable solutions.

A model is developed to describe protein release kinetics from injectable, polymer solution depots which undergo rapid phase inversion on injection. The model consists of a polymer-rich phase and a solvent-rich phase, consistent with experimentally observed phase inversion morphology. Equations in the polymer-rich phase are based on diffusion-reaction mass balances for solvent, water and dissolved drug, and the rate of dissolution of dispersed drug particles. Equations in the water-rich phase are also of the diffusion-reaction type. Transport parameters in the polymer-rich phase are coupled to the ternary thermodynamics through friction formalism, and remaining parameters are estimated from literature data, leaving two free parameters: volume fraction of water-rich phase (epsilon) and k, the mass-transfer coefficient for bath-side transfer of the protein. Variations of these parameters lead to predictions of release profiles that vary from a rapid, burst-like behavior followed by a locking-in of the polymer-rich phase, to a uniform, zero-order profile. Comparisons are made to lysozyme release data for three systems: PLGA solutions in N-methlypyrollidinone (NMP), PLA solutions in NMP, and the latter with added Pluronic. Good agreement between model predictions and data is shown; in particular, the transition from rapid release to zero-order kinetics that occurs on addition of Pluronic is illustrated.

Drug Delivery Systems↗

Formation and decay of vortex lattices in Bose-Einstein condensates at finite temperatures.

The dynamics of vortex lattices in stirred Bose-Einstein condensates have been studied at finite temperatures. The decay of the vortex lattice was observed nondestructively by monitoring the centrifugal distortions of the rotating condensate. The formation of the vortex lattice could be deduced from the increasing contrast of the vortex cores observed in ballistic expansion. In contrast to the decay, the formation of the vortex lattice is insensitive to temperature change.

Journal Article↗

Experimental observation of the Bogoliubov transformation for a Bose-Eeinstein condensed gas.

Phonons with wave vector q/(planck constant) were optically imprinted into a Bose-Einstein condensate. Their momentum distribution was analyzed using Bragg spectroscopy with a high momentum transfer. The wave function of the phonons was shown to be a superposition of +q and -q free particle momentum states, in agreement with the Bogoliubov quasiparticle picture.

Journal Article↗

Vortex nucleation in a stirred Bose-Einstein condensate.

We studied the nucleation of vortices in a Bose-Einstein condensate stirred by a laser beam. The vortex cores were observed using time-of-flight absorption imaging. Depending on the stirrer size, either discrete resonances or a broad response was visible as the stir frequency was varied. Stirring beams small compared to the condensate size generated vortices below the critical rotation frequency for the nucleation of surface modes, suggesting a local mechanism of generation. In addition, we observed the centrifugal distortion of the condensate due to the rotating vortex lattice and found evidence for bent vortices.

Journal Article↗

Realization of Bose-Einstein condensates in lower dimensions.

Bose-Einstein condensates of sodium atoms have been prepared in optical and magnetic traps in which the energy-level spacing in one or two dimensions exceeds the interaction energy between atoms, realizing condensates of lower dimensionality. The crossover into two-dimensional and one-dimensional condensates was observed by a change in aspect ratio and by the release energy converging to a nonzero value when the number of trapped atoms was reduced.

Journal Article↗

CD45 function is regulated by an acidic 19-amino acid insert in domain II that serves as a binding and phosphoacceptor site for casein kinase 2.

In this study experiments were conducted to elucidate the physical/functional relationship between CD45 and casein kinase 2 (CK2). Immunoprecipitation experiments demonstrated that CK2 associates with CD45 and that this interaction is inducible upon Ag receptor cross-linking in B and T cell lines as well as murine thymocytes and splenic B cells. However, yeast two-hybrid analysis failed to demonstrate a physical interaction between the individual CK2 alpha, alpha', or beta subunits and CD45. In contrast, a yeast three-hybrid assay in which either CK2 alpha and beta or alpha' and beta subunits were coexpressed with the cytoplasmic domain of CD45, demonstrated that both CK2 subunits are necessary for the interaction with CD45. Experiments using the yeast three-hybrid assay also revealed that a 19-aa acidic insert in domain II of CD45 mediates the physical interaction between CK2 and CD45. Structure/function experiments in which wild-type or mutant CD45RA and CD45RO isoforms were expressed in CD45-deficient Jurkat cells revealed that the 19-aa insert is important for optimal CD45 function. The ability of both CD45RA and CD45RO to reconstitute CD3-mediated signaling based on measurement of calcium mobilization and mitogen-activated protein kinase activation was significantly decreased by deletion of the 19-aa insert. Mutation of four serine residues within the 19-aa insert to alanine affected CD45 function to a similar extent compared with that of the deletion mutants. These findings support the hypothesis that a physical interaction between the CD45 cytoplasmic domain and CK2 is important for post-translational modification of CD45, which, in turn, regulates its catalytic function.

Animals↗

Observation of vortex lattices in Bose-Einstein condensates.

Quantized vortices play a key role in superfluidity and superconductivity. We have observed the formation of highly ordered vortex lattices in a rotating Bose-condensed gas. These triangular lattices contained over 100 vortices with lifetimes of several seconds. Individual vortices persisted up to 40 seconds. The lattices could be generated over a wide range of rotation frequencies and trap geometries, shedding light on the formation process. Our observation of dislocations, irregular structure, and dynamics indicates that gaseous Bose-Einstein condensates may be a model system for the study of vortex matter.

Journal Article↗

Observation of superfluid flow in a bose-einstein condensed Gas

We have studied the hydrodynamic flow in a Bose-Einstein condensate stirred by a macroscopic object, a blue-detuned laser beam, using nondestructive in situ phase contrast imaging. A critical velocity for the onset of a pressure gradient has been observed, and shown to be density dependent. The technique has been compared to a calorimetric method used previously to measure the heating induced by the motion of the laser beam.

Journal Article↗

Surface excitations of a bose-einstein condensate

Surface modes in a Bose-Einstein condensate of sodium atoms have been studied. We observed excitations of standing and rotating quadrupolar and hexadecapolar modes. The modes were excited with high spatial and temporal resolution using the optical dipole force of a rapidly scanning laser beam. This novel technique is very flexible and should be useful for the study of rotating Bose-Einstein condensates and vortices.

Journal Article↗

Differential CD5-dependent regulation of CD5-associated CK2 activity in mature and immature T cells: implication on TCR/CD3-mediated activation.

CD5 attenuates TCR-induced signals in immature thymocytes but functions as a costimulatory molecule potentiating TCR/CD3-mediated activation in mature, peripheral T cells. We have recently shown that the serine/threonine kinase, casein kinase 2 (CK2), a major regulator of cell growth and signaling, associates with and is activated by CD5. Therefore, we tested the possibility that differential regulation of CK2 activity by CD5 may be associated with these differences in CD5 modulation of TCR signaling. Consistent with our hypothesis, CD5-specific cross-linking activated associated CK2 in thymocytes but not active in mature splenocytes. Differential regulation of CD5-associated CK2 provides, for the first time, a potential mechanism for the differential effects of CD5 signaling in immature and mature cells. We propose that CD5 modulates Ag receptor activity through developmentally regulated activation of CD5-associated CK2.

Amino Acid Sequence↗

Regulation of casein kinase 2 by direct interaction with cell surface receptor CD5.

The transmembrane protein CD5, expressed on all T cells and the B1 subset of B cells, modulates antigen receptor-mediated activation. We used the yeast two-hybrid system to identify proteins that interact with its cytoplasmic domain and play a role in CD5 proximal signaling events. We found that the beta subunit of the serine/threonine kinase casein kinase 2 (CK2) interacts specifically with the cytoplasmic domain of CD5. Co-immunoprecipitation experiments showed activation-independent association of CK2 with CD5 in human and murine B and T cell lines and murine splenocytes. The interaction of CK2 holoenzyme with CD5 is mediated by the amino terminus of the regulatory subunit beta. CK2 binds and phosphorylates CD5 at the CK2 motifs flanked by Ser459 and Ser461. Cross-linking of CD5 leads to the activation of CD5-associated CK2 in a murine B-lymphoma cell line and a human T-leukemia cell line and is independent of net recruitment of CK2 to CD5. In contrast, CK2 is not activated following cross-linking of the B cell receptor complex or the T cell receptor complex. This direct regulation of CK2 by a cell surface receptor provides a novel pathway for control of cell activation that could play a significant role in regulation of CD5-dependent antigen receptor activation in T and B cells.

Amino Acid Sequence↗

Cloning and characterization of HUPF1, a human homolog of the Saccharomyces cerevisiae nonsense mRNA-reducing UPF1 protein.

Levels of most nonsense mRNAs are normally reduced in prokaryotes and eukaryotes when compared with that of corresponding functional mRNAs. Genes encoding polypeptides that selectively reduce levels of nonsense mRNA have so far only been identified in simple eukaryotes. We have now cloned a human cDNA whose deduced amino acid sequence shows the highest degree of homology to that of UPF1, a bona fide Saccharomyces cerevisiae group I RNA helicase required for accelerated degradation of nonsense mRNA. Based on the total sequence of the shorter yeast UPF1 protein, the overall identity between the human protein and UPF1 is 51%. Besides NTPase and other RNA helicase consensus motifs, UPF1 and its human homolog also share similar putative zinc finger motifs that are absent in other group I RNA helicases. Northern blot analysis with the human cDNA probe revealed two transcripts in several human cell lines. Further, antibodies raised against a synthetic peptide of the human polypeptide detected a single 130 kDa polypeptide on Western blots from human and mouse cells. Finally, immunofluorescence and Western blot analyses revealed that the human and mouse polypeptides, like yeast UPF1, are expressed in the cytoplasm, but not in the nucleus. We have thus identified the first mammalian homolog of yeast UPF1, a protein that regulates levels of nonsense mRNA, and we tentatively name this protein human HUPF1 (for human homolog of UPF1).

Amino Acid Sequence↗

Sequence of the rabbit glyceraldehyde-3-phosphate dehydrogenase-encoding cDNA.

Two full-length cDNA clones encoding rabbit glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were isolated from a lambda gt10 rabbit spleen cDNA library and sequenced. As predicted from the open reading frame (ORF) in vitro translation of a sense orientation GAPDH cDNA clone yielded a protein product with a molecular mass of 37 kDa. Rabbit GAPDH exhibits a high degree of homology to the mouse, rat, hamster, chicken and human GAPDH on both the nucleotide (nt) and amino acid (aa) levels.

Amino Acid Sequence↗

Preferential VH gene usage in rabbit Ig-secreting heterohybridomas.

B cells from leukemic rabbits preferentially use a single VH gene, VH1, in their VDJ gene rearrangements. To determine whether Ig-secreting B cells from normal rabbits also preferentially use VH1, we generated rabbit X mouse heterohybridomas that stably secreted rabbit Ig that expressed VHa allotypic specificities and analyzed the VH genes used in their VDJ gene rearrangements. We cloned the VDJ genes from nine heterohybridomas, and by comparing the restriction map of the DNA immediately 5' of the translational start site of these VDJ genes to that of the same region 5' of VH1, we showed that eight of the nine heterohybridoma clones use VH1. Comparison of the nucleotide sequences of the eight VH1-using VDJ genes with the nucleotide sequence of germ-line VH1 showed that each of them had somatically diversified. The diversified regions included clustered nucleotide changes and codon insertions and deletions, such as would be expected if the diversification process involved somatic gene conversion. We searched our database of germ-line VH genes for genes that could serve as donors for the gene conversion events, and we identified potential VH donor genes for five regions of diversification. One of these regions of diversification spanned at least 132 bp and included a codon insertion as well as 15 nucleotide changes. The data confirm that Ab diversity may be generated by a somatic gene conversion-like mechanism. The results directly demonstrate that Ig-secreting B cells from normal rabbits preferentially use VH1 in their VDJ gene rearrangements.

Amino Acid Sequence↗

CD5+ B cells predominate in peripheral tissues of rabbit.

Restricted usage of VH genes is observed in rabbit B lymphocytes and in human and murine CD5 B lymphocytes. This observation raised the possibility that most rabbit B lymphocytes were CD5+. To investigate this we cloned the CD5 gene from a rabbit cosmid library, using a probe derived from human CD5 cDNA. The rabbit CD5 gene was transfected into a murine T cell line and then we used the transfectants to develop anti-rabbit CD5 mAb. By Western blot analysis, the mAb reacted with a 67-kDa protein in lysates prepared from mesenteric lymph node and spleen cells. We determined the frequency of CD5+ B lymphocytes in peripheral lymphoid tissues of adult rabbits by two-color immunofluorescence analysis using anti-CD5 mAb and anti-L chain antibodies. The analysis showed that essentially all peripheral B lymphocytes in adult rabbits express CD5. The observation that CD5 is expressed on nearly all rabbit B lymphocytes contrasts markedly to mouse and human, where only a small number of B lymphocytes express CD5. We propose that most peripheral B lymphocytes in rabbit, as in chicken, develop early in ontogeny and are maintained throughout life by a self-renewing process.

Animals↗