[Relations between Danubian familial endemic nephropathy and its amyloid].
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Adult↗
Biomedical subjects
C Rau
Publications and source records attributed to C Rau.
[Gallbladder cancer revealed by a jaundice caused by an endobiliary tumor thrombus].
Main bile duct neoplasic thrombosis is a rare cause of jaundice in case of gallbladder cancer. We report the case of 27-year-old woman in whom the endoluminal biopsy of biliary thrombus confirmed the suspected diagnosis of gallbladder cancer. An initial laparoscopic exploration found a localized peritoneal carcinomatosis. However, in this exceptional situation with an unknown prognostic, a surgical procedure has been performed including hepatectomy IV-V with biliary principal bile duct removal, hepatico-jejunal anastomosis (Roux-en-Y), with complete resection of localized peritoneal carcinomatosis. Post-operative course were uneventful and this patient was asymptomatic under chemotherapy with a six month follow-up.
Adult↗
The significance of positive controls in testing the oncogenic potential of cell material for virus vaccine production.
For all the parts involved in the production and control of virus vaccine it is important that controls of the cell material should be carried out under optimal conditions for the detection of any abnormal characteristics of the cells. The experiments reported here attempt to establish some of the optimal parameters for testing out the tumor-inducing properties of a human diploid cell culture. All experiments were carried out on 6-week old Syrian hamsters and the main objectives were: (i) to devise an optimal immunosupression scheme and (ii) to find a more suitable positive control for testing the oncogenic potential of the human diploid cells. The anti-hamster lymphocyte serum proved to be a more potent immunosuppresser than hydrocortisone succinate as seen from the development of tumors following injections with KB cells; this scheme was subsequently used in assessing the usefulness of KB and Swiss-12 cells as positive controls. The experiments lend support to the suggestion that a heteroploid fibroblastic culture (such as Swiss-12) should become a standard positive control since not only are the tumors so obtained larger and less prone to early necrosis but, more important, they bear a closer resemblance to the kind of tumors likely to be induced by the diploid cell cultures being tested.
Animals↗
The contribution of cytochemistry and electron microscopy to the detection of contaminants in the cell material used for the production of virus vaccines.
For the production of virus vaccine it is essential to use cellular material free of contaminants that could reach the final product. It is also important to check initial tissue for possible inherent infections. Studies on primary culture of chick embryo fibroblasts have shown in several cases that cultures which appeared normal by current cytological methods had a strongly positive reaction when investigated by the Feulgen reaction for nuclear DNA and acridine orange method proving intense RNA synthesis. Comparison of electron microscopic (EM) pictures of cell sections with results obtained from negatively stained preparations of identical cell material after pronase digestion have shown the presence of viruses, thus elucidating the nature of the inclusions. The combined approach of the above-mentioned problem by cytochemical and EM methods can usefully enlarge the rage of tests employed for the definition of cell populations acceptable for virus vaccine production.
Cells, Cultured↗