Environmental pollution and health.
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Air Pollutants↗
Biomedical subjects
C Read
Publications and source records attributed to C Read.
High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro.
The first high resolution maps of the Xenopus laevis ribosomal promoter and its flanking regions (-179 to +14) have been created by assaying point mutants both in oocyte and in vitro. Within the promoter boundaries (-141(-145) to +3(+4)), domains analogous to the Core Promoter and "Upstream Control Element" (UCE) were clearly detected. The base pairs at -133, within the UCE, and -20, -10, -7, and +3, within the Core, were all shown to be especially important for promotion. Between the Core and UCE, two central promoter elements (CPEs) were also resolved. Surprisingly, these CPEs did not correspond to the highly conserved enhancer homology (approximately -70 to -110), but to CCCGGCC motifs immediately flanking it. Although xUBF was shown to be a limiting component for in vitro transcription, none of the point mutations was found to affect the interaction of this factor with the promoter.
Animals↗
Speech analysis systems: an evaluation.
Performance characteristics are reviewed for seven systems marketed for acoustic speech analysis: CSpeech, CSRE, ILS-PC, Kay Elemetrics model 5500 Sona-Graph, MacSpeech Lab II, MSL, and Signalyze. The characteristics reviewed include system components, basic capabilities (signal acquisition, waveform operations, analysis, and other functions), documentation, user interface, data formats and journaling, speed and precision of spectral analysis, and speed and precision of fundamental frequency analysis. Basic capabilities are also tabulated for three recently introduced systems: the Sensimetrics SpeechStation, the Kay Elemetrics Computerized Speech Lab (CSL), and the LSI Speech Workstation. In addition to the capability and performance summaries, this article offers suggestions for continued development of speech analysis systems, particularly in data exchange, journaling, display features, spectral analysis, and fundamental frequency analysis.
Diagnosis, Computer-Assisted↗
Point mutation analysis of the Xenopus laevis RNA polymerase I core promoter.
The core region of the Xenopus laevis pre-ribosomal RNA promoter was subjected to point mutation analysis. A total of 27 point mutants within a 78 base pair region from -64 to +14, (relative to the start of transcription at +1), were assayed by oocyte microinjection. The results locate the 3' boundary of the core promoter at +4 and the 5' boundary at between -33 and -39 and suggest that this region of the Xenopus promoter is generally similar in organisation to mammalian core promoters. In particular, the conserved guanidine nucleotides at -7 and -16 are clearly essential for promoter function. The data suggest that interactions between the transcription machinery and the promoter occur in four distinct regions around +2 to +4, -7, -17 to -20 and -28 to -33. This particular periodicity of functionally important nucleotides is consistent with a model in which all protein-DNA interactions take place from predominantly one side of the DNA helix.
Animals↗
Speech analysis systems: a survey.
Recently developed, relatively inexpensive systems make digital speech analysis available to many teachers, clinicians, and researchers who have not had access to previous systems. Addressing these prospective users, we survey five microcomputer programs and two dedicated devices for recording, editing, and analyzing speech. We review the capabilities, requirements, and user interface of each system. We recommend considerations for prospective users and for developers of the next generation of such programs and devices. Finally, we briefly survey seven additional systems.
Computers↗
The hospital trauma team: a model for trauma management.
A triage system was established as the initiating mechanism for a trauma team response to assist the assessment and early management of patients presenting to an accident and emergency department. A checklist of triage criteria was used. During a 6-month period, 342 patients (29.7% of trauma admissions) satisfied the triage criteria, which should have resulted in an average of 1.9 trauma team calls per day. Staff compliance with the triage tool was 75.4%. The overtriage rate for the checklist criteria was 52.7%. The triage tool identified patients with severe injuries who were not initially considered sufficiently injured to justify initiation of the trauma team response. The sensitivity of the triage tool in identifying patients with serious injury was 95.0%. Comparison of the review with a similar review performed 12 months earlier demonstrated that staff compliance with initiating the trauma team response had improved. Using data from 564 patients from both series, logistic regression analysis of the power of the triage criteria to predict serious injury contributed to a revision of the triage criteria. This trauma triage tool and trauma team response constitute a valid approach to the early hospital management of trauma patients. This system may be more appropriate or achievable in many hospitals than the construction of dedicated trauma reception units or permanent surgical staffing of general Accident and Emergency departments.
Adolescent↗