Biomedical subjects

C Riddell

Publications and source records attributed to C Riddell.

At least 19 recordsLinked to original sources

PubMed2005

Newborns with C8-acylcarnitine level over the 90th centile have an increased frequency of the common MCAD 985A>G mutation.

Medium chain acyl-coenzyme A dehydrogenase (MCAD) deficiency is the most commonly inherited defect of fatty acid oxidation. This autosomal recessive disorder is characterized by the tendency to become profoundly hypoglycaemic under fasting stress conditions, leading to lethargy, coma, brain injury and/or death. The most common mutation resulting in MCAD deficiency ascertained through individuals of northern European descent presenting with clinical symptoms is a single base-pair change (985A>G) that accounts for up to 90% of these abnormal alleles. In the general Nova Scotia population, which comprises largely individuals of northern European descent, this mutation is present at a frequency of 1 in 68. A recently implemented newborn screening programme for MCAD deficiency uses tandem mass spectrometry (MS/MS) to analyse blood spots from newborns for C8-acylcarnitine. After reviewing initial data from this newborn screening programme, we hypothesized that there was an unexpectedly high frequency of individuals with an MCAD 985A>G mutation and C8-acylcarnitine levels at the upper end of the normal range. A sample representing the upper 90th centile was screened for the presence of the 985A>Gmutation and 61 heterozygotes were identified, establishing a high frequency (1/10) of this 985A>G mutation in this selected population. We have demonstrated a relationship between heterozygosity for 985A>G and C8-acylcarnitine levels. These results contribute to the interpretation of C8-acylcarnitine levels and the establishment of a more clinically relevant screening cut-off point.

Acyl-CoA Dehydrogenase↗

PubMed2003

Predictive, pre-natal and diagnostic genetic testing for Huntington's disease: the experience in Canada from 1987 to 2000.

Predictive and pre-natal testing for Huntington's Disease (HD) has been available since 1987. Initially this was offered by linkage analysis, which was surpassed by the advent of the direct mutation test for HD in 1993. Direct mutation analysis provided an accurate test that not only enhanced predictive and pre-natal testing, but also permitted the diagnostic testing of symptomatic individuals. The objective of this study was to investigate the uptake, utilization, and outcome of predictive, pre-natal and diagnostic testing in Canada from 1987 to April 1, 2000. A retrospective design was used; all Canadian medical genetics centres and their affiliated laboratories offering genetic testing for HD were invited to participate. A total of 15 of 22 centres (68.2%), currently offering or ever having offered genetic testing for HD, responded, providing data on test results, demographics, and clinical history. A total of 1061 predictive tests, 15 pre-natal tests, and 626 diagnostic tests were performed. The uptake for predictive testing was approximately 18% of the estimated at-risk Canadian population, ranging from 12.5% in the Maritimes to 20.7% in British Columbia. There appears to have been a decline in the rate of testing in recent years. Of the predictive tests, 45.0% of individuals were found to have an increased risk, and a preponderance of females (60.2%) sought testing. A greater proportion of those at < or = 25% risk sought predictive testing once direct CAG mutation analysis had become available (10.9% after mutation analysis vs 4.7% before mutation analysis, p = 0.0077). Very few pre-natal tests were requested. Of the 15 pre-natal tests, 12 had an increased risk, resulting in termination of pregnancy in all but one. Diagnostic testing identified 68.5% of individuals to be positive by mutation analysis, while 31.5% of those with HD-like symptoms were not found to have the HD mutation. The positive diagnostic tests included 24.5% of individuals with no known prior family history of HD.

Adolescent↗

PubMed2002

Influence of whole barley and grit on live performance and health of turkey toms.

Live performance to 96 d was compared for 1,584 turkey toms reared on diets containing four levels of whole barley and two levels of insoluble grit (0 or 9 g/bird per wk). Nutrient specifications for all diets were similar. The six dietary treatments were 1) 0% whole barley plus grit, 2) Treatment 1 minus grit, 3) grit plus 5% whole barley at 0 d increasing to 35% by 19 d, 4) grit plus 5% whole barley at 0 d increasing to 50% by 19 d, 5) Treatment 4 minus grit, and 6) grit plus 5% whole barley at 19 d increasing to 50% by 40 d. The concentrate blended with the whole barley was fed as crumbles or pellets. Nutrients were not diluted. Compared to the control treatments, feeding 35% or more whole barley temporarily reduced weight gain and increased feed:gain prior to 68 d. Cumulative weight gain was reduced in Treatments 3 and 5 compared to treatments in which no barley was fed. Cumulative feed:gain was increased in Treatment 5 compared to Treatments 2 and 3. Total mortality and leg and skeletal problems were reduced in treatments where whole grain slowed early growth rate. Feeding grit had no effect on the live performance of birds fed similar levels of whole barley. In treatments in which whole barley was introduced at 0 d, gizzard pH was decreased at 18 d, and gizzard weight was increased at 32 d. Jejunal viscosity was unaffected by dietary treatment.

Animal Feed↗

PubMed2002

Feeding broiler chickens wheat and barley diets containing whole, ground and pelleted grain.

The effect of grain form (whole, mash, or pelleted) on the live performance of broiler chickens was determined. In the first trial, six regimens compared the feeding of whole wheat: 1) 0% of diet for the whole trial (0 to 48 d); 2) 5% at 6 d, 20% at 13 d, 35% at 27 d; 3) 5% at 6 d, 35% at 13 d, 50% at 27 d; 4) 5% at 0 d, 20% at 6 d, 35% at 13 d, 50% at 27 d; 5) 5% at 6 d, 50% at 13 d, 65% at 27 d; and 6) 5% at 0 d, 20% at 6 d, 50% at 13 d, 65% at 27d. Each feeding regimen was replicated with steam-pelleted and mash supplements. None of the feed was diluted. The second trial was similar, except that whole barley was fed instead of whole wheat. Feeding mash supplements slowed growth at all ages and lowered mortality caused by sudden death syndrome and ascites plus right heart failure. Cumulative feed:gain was increased by feeding mash supplements in Trial 1. Total weight gain was unaffected by feeding whole wheat but was decreased by most levels of whole barley. Whole-grain diets increased cumulative feed:gain. Feeding whole wheat decreased skeletal problems. Whole-grain diets increased gizzard size but did not alter carcass yield. Feeding whole-grain and mash supplements caused at least a temporary loss in growth rate and feed efficiency but in some cases improved bird health.

Animal Feed↗

PubMed2001

Noninvasive estimation of the aorta input function for measurement of tumor blood flow with.

Quantitative measurement of tumor blood flow with [15O]water can be used to evaluate the effects of tumor treatment over time. Since quantitative flow measurements require an input function, we developed the profile fitting method (PFM) to measure the input function from positron emission tomography images of the aorta. First, a [11C]CO scan was acquired and the aorta region was analyzed. The aorta diameter was determined by fitting the image data with a model that includes scanner resolution, the measured venous blood radioactivity concentration, and the spillover of counts from the background. The diameter was used in subsequent fitting of [15O]water dynamic images to estimate the aorta and background radioactivity concentrations. Phantom experiments were performed to test the model. Image quantification biases (up to 15%) were found for small objects, particularly for those in a large elliptical phantom. However, the bias in the PFM concentration estimates was much smaller (2%-6%). A simulation study showed that PFM had less bias and/or variability in flow parameter estimates than an ROI method. PFM was applied to human [11C]CO and [15O]water dynamic studies with left ventricle input functions used as the gold standard. PFM parameter estimates had higher variability than found in the simulation but with minimal bias. These studies suggest that PFM is a promising technique for the noninvasive measurement of the aorta [15O]water input function.

Aorta↗

PubMed2001

Phenotypic and genotypic characterization of virulence factors of Escherichia coli isolated from broiler chickens with simultaneous occurrence of cellulitis and other colibacillosis lesions.

The objective of this study was to characterize virulence factors of Escherichia coli isolates from broilers with simultaneous occurrence of cellulitis and other colibacillosis lesions. Thirty flocks were sampled and 237 birds with cellulitis were examined. Eighty-two (34.6%) of 237 birds condemned for cellulitis had gross lesions in the heart, air sacs, joints, or liver. In 58 chickens, E. coli was isolated from both the cellulitis and other lesions of colibacillosis, and 18.9% of the E. coli isolates from the 2 types of lesions belonged to the same O group. Escherichia coli of serogroups O78, O1, and O2 predominated. Isolates of the same serogroup that were derived from different lesions in the same birds had similar patterns of biotype, aerobactin production, serum sensitivity profile, antibiotic sensitivity, and K1 capsule production. Escherichia coli derived from cellulitis lesions produced virulence factors similar to those found in E. coli isolated from other colibacillosis lesions in poultry.

Animals↗

PubMed2001

Noise reduction in oncology FDG PET images by iterative reconstruction: a quantitative assessment.

UNLABELLED: Tumor detection depends on the contrast between tumor activity and background activity and on the image noise in these 2 regions. The lower the image noise, the easier the tumor detection. Tumor activity contrast is determined by physiology. Noise, however, is affected by many factors, including the choice of reconstruction algorithm. Previous simulation and phantom measurements indicated that the ordered-subset expectation maximization (OSEM) algorithm may produce less noisy images than does the usual filtered backprojection (FBP) method, at equivalent resolution. To see if this prediction would hold in actual clinical situations, we quantified noise in clinical images reconstructed with both OSEM and FBP. METHODS: Three patients (2 with colon cancer, 1 with breast cancer) were imaged with FDG PET using a "gated replicate" technique that permitted accurate measurement of noise at each pixel. Each static image was acquired as a gated image sequence, using a pulse generator with a 1-s period, yielding 40 replicate images over the 10- to 15-min imaging time. The images were or were not precorrected for attenuation and were reconstructed with both FBP and OSEM at comparable resolution. From these data, images of pixel mean, SD, and signal-to-noise ratio (S/N) could be produced, reflecting only noise caused by the statistical fluctuations in the emission process. RESULTS: Noise did not vary greatly over each FBP image, even when image intensity varied greatly from one region to the next, causing S/N to be worse in low-activity regions than in high-activity regions. In contrast, OSEM had high noise in hot regions and low noise in cold regions. OSEM had a much better S/N than did FBP in cold regions of the image, such as the lungs (in the attenuation-corrected images), where improvements in S/N averaged 160%. Improvements with OSEM were less dramatic in hotter areas such as the liver (averaging 25% improvement in the attenuation-corrected images). In very hot tumors, FBP actually produced higher S/Ns than did OSEM. CONCLUSION: We conclude that OSEM reconstruction can significantly reduce image noise, especially in relatively low-count regions. OSEM reconstruction failed to improve S/N in very hot tumors, in which S/N may already be adequate for tumor detection.

Algorithms↗

PubMed1999

An automatic classification technique for attenuation correction in positron emission tomography.

In this paper a clustering technique is proposed for attenuation correction (AC) in positron emission tomography (PET). The method is unsupervised and adaptive with respect to counting statistics in the transmission (TR) images. The technique allows the classification of pre- or post-injection TR images into main tissue components in terms of attenuation coefficients. The classified TR images are then forward projected to generate new TR sinograms to be used for AC in the reconstruction of the corresponding emission (EM) data. The technique has been tested on phantoms and clinical data of brain, heart and whole-body PET studies. The method allows: (a) reduction of noise propagation from TR into EM images, (b) reduction of TR scanning to a few minutes (3 min) with maintenance of the quantitative accuracy (within 6%) of longer acquisition scans (15-20 min), (c) reduction of the radiation dose to the patient, (d) performance of quantitative whole-body studies.

Brain↗

PubMed1998

Can I drive after those drops, doctor?

PURPOSE: To document the experience of patients driving after drops to dilate the pupils. There are no documented guidelines on driving after pupillary dilatation. This is a study of patients who drove a car after attending the eye casualty for an ocular examination during which their pupils were dilated. METHODS: Thirty patients were identified who felt confident to drive after their pupils were dilated. Distant visual acuities, colour vision and confrontation visual fields were assessed before and after pupillary dilatation with 2.5% phenylephrine and 1% tropicamide. These patients met the legal requirements for driving after pupillary dilatation. The patients completed a questionnaire that recorded their subjective experiences of driving while their pupils were dilated. RESULTS: The mean age of the patients was 51.9 +/- 19.7 years (range 20-73 years). Twenty-seven of the 30 patients undertook the journey on familiar roads, and 14 of 30 wore spectacles. The mean spherical equivalent was +2.00 dioptre sphere for distance and a mean additional spherical equivalent of 1.75 dioptre sphere for near. Twenty patients experienced glare, which was severe enough to cause difficulty driving in three cases. Two of these patients drove in sunny weather conditions and one in light (not sunny) weather. Six patients had difficulty with road signs, two had difficulty judging distances and one reported difficulty with traffic lights. CONCLUSIONS: Dilating the pupils did not reduce distance visual acuity. However, it would seem appropriate to warn patients of the problems associated with glare and, if driving is their only option for transport home, recommend that they allow sufficient time to adapt to a dilated pupillary state, drive only on familiar roads and avoid driving in sunny weather.

Adult↗

PubMed1997

A study of electrocardiographic patterns in a population of commercial broiler chickens.

A study was completed to characterize the electrocardiographic (ECG) patterns of male broiler chickens. Data were collected from 300 commercial broilers. ECG readings were collected from all birds between 12 and 15 days of age and then twice more at 10-day intervals. The measurements. included heart rate and rhythm, QRS complex duration, amplitude and mean electrical axis (MEA), incidence of ascites and incidence of sudden death syndrome (SDS). Eight birds died from SDS and 4 birds died from ascites. Twelve birds were condemned for ascites at the processing plant. The overall population heart rate declined with age. Birds that died of SDS had a higher heart rate, whereas those that developed ascites had a lower heart rate than the remainder of the population. The normal MEA was found to be between 0 degree and 180 degrees. On average 30% of birds showed left or right QRS axis deviation, and this pattern was observed in 14 of the 16 birds that developed ascites. Several types of cardiac arrhythmias were observed, the most common being premature ventricular contractions (PVC). The incidence of PVC increased with age, ranging from 1% at 12-15 days of age to 8.9% at 32-35 days of age. QRS axis deviation was present in 5 SDS birds. It is concluded that some 30% of the broiler flock tested was at risk of developing heart failure or heart-related disease.

Aging↗

PubMed1997

Evaluation of pre- and post-reconstruction count-dependent Metz filters for brain PET studies.

In this study we evaluated pre- (PR-R) and post-reconstruction (PO-R) count-dependent Metz filters for PET brain studies in order to increase signal-to-noise ratio. A set of studies using a 3D Hoffman brain phantom was performed at various count levels, and a reference image set was created from extremely high count images. Several combinations of PR-R and PO-R filtering were considered to find the optimal means of processing, including: Hann filter alone; PR-R Metz filter without or with a PO-R low pass filter; and PO-R Metz filter without or with a PR-R low pass filter. A formula was established to correlate the optimal PO-R Metz filter order with the net counts. Resolution [full width at half-maximum (FWHM) and fill width at tenth maximum (FWTM)], normalized residual mean square differences (NRMSD) between the ideal and the processed images, noise reduction and contrast were used as parameters for the evaluation of the different filter combinations. Resolution is decreased by all filter combinations that can effectively control noise; however, FWTM increases less than FWHM. NRMSD indicates that the use of Hann and (optimal) PO-R Metz filter is the most powerful combination from among those tested. A close correlation (r = 0.969) was found between the net counts and the optimal order of the PO-R Metz filter. At the count levels of clinical studies the PO-R Metz filter was found to control noise much more effectively and enhance the contrast when compared to the routinely used Hann filter alone, and produced images of better quality.

Biophysical Phenomena↗

PubMed1997

Isolation of Escherichia coli from cellulitis and other lesions of the same bird in broilers at slaughter.

Cellulitis results in substantial losses to the broiler industry due to condemnations at slaughter. This study was conducted to clarify the association between Escherichia coli isolated from cellulitis and other lesions caused by E. coli in individual birds. Fourteen flocks were sampled and 118 birds with cellulitis were examined. Escherichia coli was isolated from all but 2 of the cellulitis lesions, and serogroups O78, O1, and O2 predominated. Thirty-six birds had at least 1 other lesion in addition to the cellulitis lesion. Isolation of E. coli from cellulitis and other lesions occurred in 7 of the 14 flocks. Escherichia coli of the same serogroup were isolated from cellulitis and other lesions in some birds, suggesting that a single E. coli may sometimes be responsible for both types of lesions.

Animals↗

PubMed1996

Escherichia coli cellulitis in broiler chickens: clonal relationships among strains and analysis of virulence-associated factors of isolates from diseased birds.

Thirty-nine Escherichia coli isolates from broiler chickens with cellulitis were serotyped and analyzed for clonal relationships by multilocus enzyme electrophoresis. The isolates were further characterized with respect to hemagglutination (HA); serum resistance; antibiotic susceptibility; production of aerobactin, colicin V, and hemolysin; expression of K1 or K5 capsule; sensitivity to cloacin DF13 after treatment with diphenylamine; expression of iron-regulated outer membrane proteins; and virulence in 1-day-old chickens. In addition, the isolates were examined for the presence of DNA sequences related to F1A (fim) and P (pap) fimbriae, aerobactin synthesis (iuc) and transport (iut), hemolysin operon hly, and TraT lipoprotein-induced serum resistance (traT). Only 38.4% of the isolates were typeable with standard O antisera, and of these, serogroups O25 and O78 were the most frequently observed. Multilocus enzyme electrophoresis, based on 20 enzymes, resolved 17 electrophoretic types, forming seven clusters. Isolates from four of these clusters fell into E. coli clone complexes that have been previously reported to be commonly associated with avian colibacillosis. All isolates expressed two to five iron-regulated outer membrane proteins, were resistant to serum and cloacin DF13, and possessed DNA sequences homologous to fim and iuc/iut. Most isolates (72%) were positive for traT, and a majority produced colicin V and aerobactin (92 and 82%, respectively). Assays for the presence of fim and pap DNA sequences, for HA, and for virulence gave variable results but suggest that cellulitis isolates may express F1A and/or other mannose-resistant HA fimbriae different from P and may be virulent in 1-day-old chickens. Our results support the hypothesis that cellulitis in broilers in many cases is caused by E. coli clones identical to other pathogenic avian E. coli strains. Certain clones may be specific to cellulitis, because 25% of the isolates tested belong to clusters not related to known clone complexes.

Animals↗

PubMed1995

The approximate inverse and conjugate gradient: non-symmetrical algorithms for fast attenuation correction in SPECT.

Hybrid methods have been known for a long time as very efficient algorithms for attenuation correction in single-photon emission computed tomography, but only recently have efforts been made to formulate them with more rigorous mathematics. This has allowed us to explain their efficiency in terms of approximate inversion, and to establish a convergence condition. The present study focuses on the convergence problem and emphasizes the question of symmetry. Hybrid method operators are not symmetrical; therefore the convergence condition is not easily verified. New schemes based on a modified conjugate gradient method are presented. Convergence is proved and performances are shown to be at least as good as the standard hybrid schemes on perfect and noisy simulated data.

Algorithms↗

PubMed1994

Effect of lighting treatment on the productivity, health, behavior and sexual maturity of heavy male turkeys.

1. Turkey poults (1620) were used to compare the effects of three lighting programmes on heavy strain males reared to 188 d: constant light (24L:0D, CON); increasing light (6L:18D at 7 d gradually increasing to 20L:4D by 63 d, INC); a pattern identical to INC followed by a decrease in daylength from 84 d to 10L:14D at 112 d (DID). 2. Lighting affected growth pattern but had no effect on body weight at 118 d or overall food to gain ratio. 3. Both INC and DID lighting reduced overall mortality in comparison to CON light primarily because of a reduction in the incidence of skeletal disease and spontaneous cardiomyopathy. INC and DID lighting increased the incidence of cannibalism. 4. Turkeys given INC or DID lighting had a superior ability to walk in comparison to those birds given CON light. 5. INC and DID males stood, ate and drank more frequently, and sat less often than CON turkeys during behavioural observation. 6. There were no lighting effects on carcase composition except that INC and DID birds had heavier keel bones. The ultimate force per cm2 (stress) required to break femora was greater for turkeys given INC and DID lighting (P = 0.065). 7. Plasma testosterone concentrations at 117 d were 272.5, 115.2 and 29.5 pg/ml for turkeys given CON, INC and DID lighting, respectively (P = 0.072). Testosterone concentration was not related to growth rate.

Animal Husbandry↗

PubMed1994

A retrospective study of circovirus infection in pigeons: nine cases (1986-1993).

Circovirus infections were diagnosed in 12 pigeons from the United States 4 pigeons from Australia, and 1 pigeon from Canada (1986-1993). Circovirus was identified by electron microscopic examination of basophilic botryoid cytoplasmic inclusions that had a histologic appearance similar to that of psittacine beak and feather disease virus inclusions. Inclusions were seen in splenic, bursal, gut-associated, and bronchus-associated lymphoid tissue macrophages and in bursal epithelial cells. Inclusions were composed of paracrystalline arrays of tightly packed, nonenveloped icosahedral virions 14-17 nm in diameter. Histologic changes in the spleens ranged from lymphofollicular hyperplasia with mild discrete lymphocellular necrosis to lymphoid depletion and diffuse histiocytosis. Lesions in the bursa of Fabricius ranged from mild lymphocellular necrosis to severe cystic bursal atrophy. Remaining histologic findings coincided with concurrent bacterial, viral, fungal, and parasitic infections. Immunoperoxidase staining and DNA in situ hybridization demonstrated that pigeon circovirus is distinct from psittacine beak and feather disease virus; however both viruses apparently share some homologous DNA sequences. Clinical and diagnostic findings indicate that pigeon circovirus may be similar to psittacine beak and feather disease virus with respect to acquired immunodeficiency and subsequent multiple secondary infections.

Animals↗

PubMed1993

Correction of attenuation in SPECT with an attenuation coefficient map: a new method.

Attenuation coefficient (mu) maps, measured from transmission scan, are now becoming available. A simple method of attenuation correction is needed for routine implementation, however. Significant attenuation compensation can be mathematically obtained by dividing each actual pixel value of emission projections by the average of all the attenuation factors [exp (-sigma mu)] of all voxels along the same projection ray. This simple method, compatible with filtered back projection algorithms, was tested on simulations of cardiac and cerebral transaxial images on a Vax computer using the RECLBL library. In the models, the different structures received different activity and mu values. Three types of emission projections were generated: the ideal projections obtained by summation of the activity along each projection ray, the corresponding attenuated projections, and the projections corrected for attenuation. Comparison of projections on a pixel by pixel basis showed differences of less than 20% between the corrected and ideal projections. After reconstruction, both absolute and relative quantification were greatly improved by the correction of attenuation. Further validation of the method is in progress with actual patient data.

Algorithms↗

PubMed1991

Effects of increasing photoperiod length on performance and health of broiler chickens.

1. Broiler performance and health were compared from 0 to 42 d for lighting programmes consisting of near-continuous light 23L:1D (23L), a lighting programme where photoperiod gradually increased from 6 to 23 h between days 4 and 35 (INC) or an increasing-lighting programme with 1 h of light mid-way through the scotophase (INC + 1). 2. The increasing lighting programmes reduced growth rate and increased gain:food ratio from 0 to 21 d; INC + 1 growth rate was intermediate to the 23 L and INC treatments. From 0 to 42 d there were no differences in growth rate or gain:food ratio among lighting treatments. Males grew faster, ate more food and converted food more efficiently than females. 3. The increasing-lighting programmes (INC and INC + 1) resulted in less skeletal disease than the 23L control. The incidence of sudden death syndrome (SDS) and mortality other than SDS were numerically lower for the increasing lighting treatments. More males died as a result of SDS and were affected by skeletal defects than females. 4. In terms of health, increasing lighting programmes were superior to near-continuous light for broiler chickens.

Animals↗