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Biomedical subjects

C Roussel

Publications and source records attributed to C Roussel.

At least 19 recordsLinked to original sources
PubMed1990

Latex enzyme immunoassay for measuring IgG antibodies to rubella virus.

A latex enzyme immunoassay (LEIA) for detecting IgG class antibody to rubella virus was compared with the latex agglutination test (LA) and a haemagglutination inhibition assay (HI). Of 243 sera tested, four discrepant results were observed among all three techniques, corresponding to borderline values. Except for one sample containing specific IgM class antibody, the difference between quantitative results from each pair of tests was always within a value corresponding to two doubling dilutions and was considered to have acceptable variation. The LEIA technique allowed 10 seroconversions to be detected, as determined by the other techniques. The LEIA required a single 1 in 8 sample dilution, took 30 minutes, and provided a useful alternative assay for the quantitation of rubella antibodies.

Antibodies, Viral↗
PubMed1989

Heterophile IgM, IgA, and IgE antibodies in infectious mononucleosis.

Fifty infectious mononucleosis (IM) and 150 non-infectious mononucleosis (non-IM) sera were tested by a hemadsorption immunocapture test (HIT) for the detection of heterophile antibodies of IgM, IgA, and IgE classes. The specificity of Paul-Bunnell (PB) antibodies was ascertained by a differential absorption test. IgM PB-antibodies were demonstrated in 100% of IM sera, IgA in 92%, and IgE in 88%. PB antibodies were present only in IM sera; but other heterophile antibodies of IgM (68%), IgA (6.7%), and IgE (9.3%) classes were demonstrated in non-IM sera. IgA and IgE heterophile antibodies were thus present, especially in IM sera. HIT was found to be more sensitive than Paul-Bunnell Davidsohn test (PBD test) and suitable for the diagnosis of infectious mononucleosis.

Antibodies, Heterophile↗
PubMed1988

The large neurofilament subunit (NF-H) of the rat: cDNA cloning and in situ detection.

A lambda gt11 expression library was screened with a polyclonal antiserum directed against Wla Wolfgram protein which corresponds to the 2',3'-cyclic nucleotide 3'-phosphohydrolase. This antiserum also recognizes epitopes of the high protein subunit of neurofilaments (NF-H). An NF-H cDNA clone (pNF-H1: 1.7 kb) was isolated and characterized. Using pNF-H1 as a probe, a second cDNA clone (pNF-H2: 3 kb) was isolated from the lambda gt11 library. The two clones were sequenced and pNF-H2 was found to encode 80% of rat NF-H protein (coil-2 and C-terminal region). The C-terminal region contains an unusual sequence with stretches of repeats of Lys-Ser-Pro which represent possible phosphorylation sites. Specific localization in neurons of the corresponding mRNA was demonstrated by in situ hybridization using the pNF-H1 as a probe.

Amino Acid Sequence↗
PubMed1988

Endocytic origin for periaxonemal vesicles along the flagellum during mouse spermiogenesis.

In mouse spermatogenesis, formation of the flagellum is associated with the presence of numerous periaxonemal vesicles. These are present in the cytoplasmic portion, limited by the deep invagination of the plasma membrane surrounding the axoneme; the number and size of these vesicles varies during spermiogenesis. The vesicles appear at step 10 in young spermatids and increase in number and size until step 14; they then rapidly decrease and disappear at step 16. Cationic ferritin (CF), an endocytic marker, directly injected in the lumen of the seminiferous tubules, labels periaxonemal vesicles, 1 hour after the injection, showing their endocytic origin. Some vesicles are membrane invaginations, still in continuity with the extracellular space, whereas others probably come from a phagocytic mechanism. The CF also shows that some vesicles flow along the axoneme and they accumulate in small cytoplasmic extensions before disappearing. All these complex endocytic phenomena go on to form certain components of the flagellum.

Animals↗
PubMed1988

Microparticle enzyme immunoassay for determination of immunoglobulin G antibodies to human cytomegalovirus.

A microparticle enzyme immunoassay (LEIA) that uses passive latex agglutination was used to detect cytomegalovirus immunoglobulin G antibodies in 495 serum samples. LEIA was in excellent concordance with latex agglutination (97%) and an enzyme-linked immunosorbent assay (98.3%). The sensitivity and specificity of LEIA compared with those of the enzyme-linked immunosorbent assay were 100 and 92.5%, respectively.

Antibodies, Viral↗
PubMed1987

Solid phase reverse passive hemadsorption test for hepatitis B surface antigen.

A solid phase reverse passive hemadsorption test (SP-RPHAd) for hepatitis B surface antigen detection is described. It was compared with a commercial reverse passive hemagglutination assay (Hepatest, Wellcome, U.K.). SP-RPHAd is four-fold less expensive than Hepatest and undiluted sera can be used instead of eight-fold diluted sera without risk of non-specific hemagglutination. Thus, the threshold of detection is lowered to about 5 ng/ml by SP-RPHAd.

Enzyme-Linked Immunosorbent Assay↗
PubMed1986

[Technical parameters influencing the reactivity of the vascular endothelium in relation to lectin of Evonymus europaeus].

Some parameters characterizing sample preparation for coloration with lectins were investigated in the model of sheep myocardial capillaries, revealed by EEA after formol fixation and paraffin embedding. The most influential factors are: addition of lysine-periodate to paraformaldehyde, duration of fixative immersion, oldness of paraformaldehyde dilution, delay of fixation (if below 8 h). All of them happen before embedding. On the other hand, those happening later are out of influence.

Animals↗
PubMed1985

[Current concepts on therapeutic possibilities in alcohology].

Therapeutic possibilities in the field of alcoholism are certainly more extensive than accepted by most general practitioners and internists whose attitude is, according to an inquiry, rather fatalistic. The actual therapy of alcoholism proves to be effective, the earlier the therapy being started the better are the results. On this account, the necessity of preventive measures and of an early treatment is to be underlined. General practitioners and internists (whose training in this area appears to be still deficient) as well as centers for nutritional hygiene and alcohology (presently about 200 consultation centers in France) may play here a cardinal role. The treatment of alcohol-addicts often justifies hospitalisation, more and more in general hospitals, where a therapeutic program, at the first place a long-term psychotherapy, is set up. The alcoholic should constantly be assisted by his treating physician, by specialised centers and also by fellowships of former alcohol-addicts.

Alcoholism↗
PubMed1979

[Nidation in the Triton W.R. 1339 treated mouse: effect of progesterone and estradiol].

In the mouse injection of 800 mg/kg of Triton W.R. 1339 during the first four days of gestation prevents nidation. Examination of the uterine horns between days 6-12 reveals that a daily injection of 5 mg progesterone up from day 1 of gestation assures the survival of the blastocysts in Triton W.R. 1339 treated females and determines a delayed nidation. Nidations occur only after injection of 0.1 microgram of oestradiol benzoate at the beginning of the 5th day.

Animals↗
PubMed1976

[Implantation defect in the mouse treated by WR 1339 triton: study after transplantation of ova].

Doses of 600 to 800 mg/kg of Triton W.R. 1339 from day 1 to 4 is very noxious in the mouse. In only 12 to 20% of animals the gestation is not interrupted. The morphology of the morulas examined on day 4 and the results of transplantation experiments on pseudopregnant mice gives evidence that Triton W.R. 1339 has no a direct toxic effect on the fertilized egg but acts probably on the maternal organism.

Animals↗
PubMed1976

[Determination of the action of WR 1339 triton on the 1st stages of gestation in the mouse: action of progesterone].

Injection of 600 to 800 mg/kg of Triton W.R. 1339 from day 1 to day 4 of pregnancy is highly noxious in the mouse. Only 12 to 20% of females continue their pregnancy. The causes of this action could be determined. Triton W.R. 1339 has not a direct toxic action on the fertilized ovum, but impairs the hormonal balance of the pregnant mouse, inducing a progesterone deficiency. Daily injection of 5 mg progesterone per animal from day 1-17 or from day 4 to 17 improve pregnancy performance or suppresses completely the noxious action of Triton W.R. 1339.

Animals↗