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C Rufener

Publications and source records attributed to C Rufener.

At least 19 recordsLinked to original sources
PubMed1977

Immunocytochemical evidence for glucagon-containing cells in the human stomach.

To determine if glucagon-containing cells could be identified in the human fundus, stomachs attained at autopsy within 4-hours of death from persons previously considered to be in good health were examined by the indirect immunoperoxidase technique using antiglucagon serum 30K. Glucagon-containing cells were demonstrated in one of eight gastric fundi examined. The glucagon content of acid alcohol extracts of the fundi examined. The glucagon content of acid alcohol extracts of the funci was low in all cases. Glucagon content was also low in canine stomach removed 4-hours after death. It is concluded that glucagon-containing cells, demonstrable by immunocytochemical techniques, may be present in the gastric fundus of humans.

3,3'-Diaminobenzidine↗
PubMed1976

Immunofluorescent localization of secretin in pancreatic monolayer culture.

Immunofluorescent cells to synthetic secretin were identified in monolayer culture of neonatal rat pancreas. No cross reaction of anti-secretin was observed with either glucagon, somatostatin or gastrin. The presence of cells containing secretin or a secretin-like peptide adds a new cell type to the three already characterized (insulin, glucagon and somatostatin containing cells) in monolayer culture.

Animals↗
PubMed1976

[Immunocytochemical identification of the glucagon-secreting cells in gastric mucosa].

Immunocytochemical investigations at the light and electron microscope level, using specific antiglucagon serum, revealed in the gastric oxyntic mucosa of the Dog, the presence of positive cells, undistinguishable ultrastructurally from pancreatic A-cells. No positive reaction was detected in any other gastrointestinal segment. These results suggest that gastric A-cells are responsible for the secretion of gastric glucagon.

Animals↗
PubMed1976

Hypertrophy and hyperplasia of somatostatin-containing D-cells in diabetes.

Insulin-, glucagon-, and somatostatin-contianing cells, identified by immunofluorescent staining, were quantitated morphometrically in sections of pancreas obtained from diabetic and nondiabetic humans and rats. Both the volume density and number of somatostatin- and glucagon-containing cells were significantly increased in the islets of juvenile-type human diabetics and of streptozotocin diabetic rats.

Adult↗
PubMed1976

Identification of glucagon-producing cells (A cells) in dog gastric mucosa.

An immunocytochemical technique using specific antiglucagon serum reveals the presence of glucagon-containing cells situated exclusively in the oxyntic glandular mucosa of the dog stomach. Electron microscope examination of the mucosa demonstrated endocrine cells containing secretory granules with a round dense core surrounded by a clear halo, indistinguishable from secretory granules of pancreatic A cells. Like the alpha granules of pancreatic A cells, the granules of these gastric endocrine cells exhibited a peripheral distribution of silver grains after Grimelius silver staining. Moreover, the granules of these cells were found to be specifically labeled with reaction product, using the peroxidase immunocytochemical technique at the ultrastructural level. Accordingly, these cells were named gastric A cells. These data suggest that the gastric oxyntic mucosa contains cells indistinguishable cytologically, cytochemically, and immunocytochemically from pancreatic A cells. It is believed that gastric A cells are responsible for the secretion of the gastric glucagon.

Animals↗
PubMed1975

Immuno-fluorescent reactivity to anti-somatostatin in the gastro-intestinal mucosa of the dog.

Evidence is presented that somatostatin-containing cells are present in the gastro-intestinal tract of the dog. Thus immuno-fluorescent cells were detected by the use of antiserum to cyclic somatostatin. These cells were mainly encountered in the antral mucosa and in the neighbourhood of gastrin-producing cells. No cross reaction was observed between gastrin and somatostatin. It is suggested that locally produced somatostatin controls gastrin secretion, and, more generally, that somatostatin-containing cells, multifocally distributed, modulates secretion of a large number of glands.

Animals↗
PubMed1975

Evidence for the D-cell of the pancreas secreting somatostatin.

By immunofluorescence, somatostatin-, glucagon- and insulin-containing cells are localized in serial sections of the pigeon pancreas. The distribution of the somatostatin immunofluorescent-cells corresponds to that of the D-cells (A1-cells), which are particularly numerous in this animal species. This observation, coupled with the finding of D, A and B-cells at the ultrastructural level, indicates that the D-cell is responsible for the secretion of somatostatin.

Animals↗