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Biomedical subjects

C Rugarli

Publications and source records attributed to C Rugarli.

106 records · Page 6Linked to original sources
PubMedDate not supplied

Impairment of lymphocyte suppressive system in recent onset insulin-dependent diabetes mellitus. Correlation with blood glucose and serum insulin levels.

In a previous study, we observed an impairment of the theophylline-induced suppressive system in recent onset IDDM patients, and demonstrated also a correlation with metabolic derangement. The aim of this study was to better investigate the relationship between theophylline sensitivity (ThS) and blood glucose/plasma insulin levels in recent onset IDDM patients subjected to preprogrammed variations by an insulin/glucose clamp with artificial pancreas. Eight patients were studied within 8 weeks from the onset of IDDM. ThS was evaluated as the ability of theophylline to inhibit blastogenic response of peripheral blood lymphocytes (PBL) to Concanavalin A (ConA), after 120 min preincubation of the cells. All patients were connected to an artificial pancreas. Through i.v. continuous insulin infusion (0.02 U/kg/h) and/or i.v. continuous glucose and saline infusion, the following experimental conditions, lasting at least 1h, were obtained: T1: relative euglycemia and normal insulinemia; T2: relative euglycemia and hyperinsulinemia; T3: hyperglycemia and normal insulinemia; T4: hyperglycemia and hyperinsulinemia. ThS was maintained in 6/8 patients at T1 and in 8/8 patients at T4. ThS was lost in 4/8 patients at T2 and T3. These data suggest that the loss of ThS induced by hyperglycemia can be corrected by hyperinsulinemia, and that it is maintained when euglycemia is accompanied by hypoinsulinemia. It is lost when these two parameters lose their interrelationship.

Activity Cycles↗
PubMedDate not supplied

B and T cell surface markers on peripheral lymphocytes from diabetic patients.

A study of T and B cell surface markers was carried out on peripheral blood lymphocytes from diabetic patients, employing immunofluorescent staining for membrane Ig, and the E rosette test. According to the immunofluorescent studies the % of sIg bearing lymphocytes decreases with age; this trend is more evident in healthy individuals. Lymphocytes from diabetic patients posses a higher amount of membrane IgG than age-matched controls. It was only in the younger diabetic patients that a definite decrease was observed in the % of IgM bearing lymphocytes. The evaluation of E rosette-forming lymphocytes revealed a similar age-associated reduction both in diabetics and in controls. Some common features of the pattern of T and B cell markers on lymphocytes from younger diabetic patients and older controls suggest that the former undergo, at least from the immunological standpoint, a more precocious ageing process. It is possible that some metabolic disorders in lymphocyte activity may play a role in this, resulting in a deficiency in the control of immunological mechanisms.

Adult↗
PubMedDate not supplied

Circulating immune complexes in patients with lung neoplasms.

A search was made for circulating immune complexes in 42 patients with biopsy-proven lung cancer; sex- and age-matched healthy volunteers were used as normal controls. Two different mthods were employed: Clq--binding assay and the conglutinin-binding test. Patients and controls were also examined for delayed hypersensitivity by a skin testing using a set of recall antigens. A significant difference was found in the incidence of immune complexes and the response to skin tests between neoplastic subjects and normal controls. However, the incidence of immune complexes was not related to delayed hypersensitivity or the other clinical features investigated.

Adenocarcinoma↗
PubMedDate not supplied

Association of lupus anticoagulant with polyarteritis nodosa: report of a case.

A patient with a documented diagnosis of polyarteritis nodosa and laboratory evidence of a circulating lupus anticoagulant is described. Additional clinical features suggestive of the antiphospholipid antibody syndrome were found. The patient underwent amputation of the first two digits of the foot due to ischemic necrosis. Steroid and immunosuppressive treatment resulted in clinical improvement and disappearance of the circulating anticoagulant, without necessitating additional treatment with oral anticoagulants. The presence of the lupus anticoagulant might have worsened the vascular damage done by polyarteritis nodosa in this patient.

Amputation, Surgical↗
PubMedDate not supplied

Age-related changes in interleukin 2 responsiveness of resting and activated human mononuclear cells.

BACKGROUND: Although the "T cell model" on the immunological decline associated with aging is gaining increasing support, the relationship between response to IL2 and aging has not yet been investigated. METHODS: Human peripheral blood mononuclear cells (PBMC) from healthy elderly donors were cultured with increasing concentrations (1-1000 U/ml) of recombinant interleukin 2 (rIL2). RESULTS: PBMC from older donors proliferated as much as those from younger donors until the fifth day of culture, but showed a reduced capability to proliferate in the following days (8th and 10th). Culturing PBMC with PHA we obtained the opposite result. In fact, the known depressed proliferative response of elderly people to the mitogen PHA is detectable only in the early days of culture. Furthermore, the response to rIL2 of 1% or 0.125% PHA-stimulated PBMC showed an important impairment of the proliferative rate in the elderly population with respect to younger controls, although no differences in the number of IL2 receptor-positive cells were detected. This phenomenon was demonstrable mainly in the first several days of culture. CONCLUSIONS: These data suggest a delay in the activation rather than a real functional impairment of PBMC from old donors.

Adolescent↗
PubMedDate not supplied

Interleukin 2 and mitogen driven proliferation in human peripheral mononuclear cells: a kinetic analysis.

The role of the T lymphocyte growth factor, interleukin 2 (IL2), on human lymphocyte proliferation was investigated. The results define the extent and the chronological sequence of IL2 effects on unstimulated mononuclear cells and on an activated population enriched in IL2 receptor positive cells. Important differences were observed in the proliferative response to the mitogen PHA when the IL2 concentration in the culture medium was varied. The data suggest that IL2 concentration is critical in the responsiveness to a mitogenic stimulus.

Adult↗
PubMedDate not supplied

Immunological status of aged subjects with reference to serological evidence of autoimmunity.

When a group of 104 aged subjects was screened for autoimmune reactions, positive reactions for the rheumatoid factor and/or autoantibodies (ANA, anti-thyroid, PCA, anti-smooth muscle, anti-mitochondria) were recorded in 40.4%. Immunological functions were studied in 32 positive aged subjects, 32 age- and sex-matched negative controls, and 32 young subjects. Some differences attributable to the process of aging were quite evident, such as a depression in the percentage of E rosette forming peripheral lymphocytes and in their response to PHA, and an increase in the percentage of IgG-bearing peripheral lymphocytes and in the serum levels of IgA and three complement fractions (C'3, C'4, and C'3-PA). No clear-cut picture was noted when autoimmunity-positive and -negative, aged subjects were compared. However, some differences between sexes suggest that autoimmune reactions are linked to a depressed T cell function mainly in males, whereas the reverse is true for females.

Adult↗