Analysis of photoenzymatic repair of UV lesions in DNA by single light flashes. VII. Photolysis of enzyme-substrate complexes in vitro.
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DNA, Bacterial↗
Biomedical subjects
C S Rupert
Publications and source records attributed to C S Rupert.
Shapes of the U.V. inactivation curves for single and linked double markers in Haemophilus influenzae transforming DNA.
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DNA, Bacterial↗
Presence of photoreactivating enzyme in blue-green algal cells.
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Darkness↗
Concerning the mechanism of formation of uv-induced thymine photoproducts in DNA.
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Journal Article↗
Addendum: concerning the mechanism of formation of UV-induced thymine photoproducts in DNA.
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DNA↗
The effects of host-cell reactivation on assay of U.V.-irradiated Haemophilus influenzae transforming DNA.
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DNA, Bacterial↗
Relation of photoreactivation to photoenzymatic repair of DNA in Escherichia coli.
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DNA, Bacterial↗
Analysis of photoenzymatic repair of UV lesions in DNA by single light flashes. I. In vitro studies with Haemophilus influenzae transforming DNA and yeast photoreactivating enzyme.
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DNA, Bacterial↗
Analysis of photoenzymatic repair of UV lesions in DNA by single light flashes. II. In vivo studies with Escherichia coli cells and bacteriophage.
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Coliphages↗
The effect of post-UV dark incubation on survival of chick-embryo fibroblasts after photoreactivation.
A survival assay with chick-embryo fibroblasts was used to study photoreactivation of ultraviolet (UV) irradiation-induced damage. The kinetics of the photoreactivation was studied as a function of the length of a post UV dark incubation period of from 0 to 18 h at 38.5 degrees C. The logarithmic survival curve with no photoreactivation had a Do of about 4.3 J/m2 giving approximately 0.8% relative plating efficiency after a UV dose of 21 J/m2. At this dose the efficiency of photoreactivation (survival increase per unit blacklight dose) increased with post UV incubation time reaching a maximum at 4-6 h, then declining until there was little photoreactivation observed for times longer than about 11 h. The possibility that this effect was produced by pre-UV perturbations of the cell cycle was eliminated by the fact that the same results were achieved after several rather different trypsinization protocols. The shape of the photoreactivation vs. blacklight curve at the time of peak efficiency showed a threshold up to about 3 kJ/m2, a rising portion and a plateau after 12-16 kJ/m2 when the survival increased by a factor of roughly 8.
Animals↗