Biomedical subjects

C Sérié

Publications and source records attributed to C Sérié.

18 recordsLinked to original sources

PubMed1984

Leishmaniasis in Greece I. Isolation and identification of the parasite causing human and canine visceral leishmaniasis.

Three human and 19 canine leishmanial stocks were typed according to their excreted factor serotype and the electrophoretic mobility of their MDH, GPI, G6PDH and 6PGDH and shown to be identical with regard to these characters and, thus with Leishmania donovani infantum. This verifies the opinion of earlier researchers, who suggested that the parasites which cause human and canine visceral leishmaniasis in Greece are the same organism and that dogs are the reservoir for the human infection. The complexities raised by the co-existence of human cutaneous leishmaniasis in Greece caused by L. tropica (formerly L. t. minor) are stressed. A comparison was made of the clinical symptomatology, serological diagnosis by IFA and ELISA tests and parasitological diagnosis of the human cases and canine infections.

Adult↗

PubMed1984

Leishmaniasis in Greece II. Isolation and identification of the parasite causing cutaneous leishmaniasis in man.

The serological and biochemical identity of four Greek leishmanial strains isolated from cases of cutaneous leishmaniasis was determined. All four strains were identical and shown to be Leishmania tropica (formerly L. t. minor). The cases are described; two came from the Greek mainland and two from Greek islands, one of the latter being a case of leishmaniasis recidivans. The significance of the results is discussed, in particular the co-existence in Greece of strains of L. tropica and L. donovani infantum.

Adult↗

PubMed1982

A controlled field trial of live Salmonella typhi strain Ty 21a oral vaccine against typhoid: three-year results.

A controlled field trial of Salmonella typhi strain Ty 21a oral vaccine against typhoid was carried out in Alexandria, Egypt, from March 1978 to March 1981. A total of 32,388 children was included in the study. The children were divided in two comparable groups, one given three doses of vaccine and the other three doses of placebo. Each dose of vaccine contained 1-8 X 10(9) live Ty 21a bacteria. The population was monitored, and each suspected case of typhoid was investigated bacteriologically and serologically. The effectiveness of the vaccine was assessed by analyzing the number of confirmed cases of typhoid fever in the two groups. The incidence of typhoid fever was 4.9 cases per 10,000 children per year in the control group and 0.2 cases per 10,000 children per year in the vaccine group. The results indicate that, in the dose schedule used, the Ty 21a mutant strain, which is stable and safe, is protective for a period of at least three years.

Administration, Oral↗

PubMed1981

Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium.

One hundred and eighty samples of pork sausages were examined after pre-enrichment in buffered peptone water (P medium), for the presence of salmonellas. From each pre-enrichment four enrichments were made: (1) o.1 ml of P medium was inoculated into 10 ml of Rappaport's medium formula R 10 (R 10/43 degrees C), /2) 1 ml of the P medium was added to 100 ml of R10 broth (R10/100 ml/43 degrees C), (3) 1 ml of P medium was inoculated into 10 ml of Muller-Kauffmann tetrathionate broth (MK medium) prepared in accordance with the International Standards Organization document ISO 3565 (MK/43 degrees C) and (4) 10 ml of P medium were added to 100 ml of MK broth (MK/100 ml/43 degrees C). All the enrichments were incubated at 43 degrees C for 48 h. Forty-six and 47 samples were found positive with the first two enrichment methods (R10/43 degrees C and R10/100 ml/43 degrees C), while only 16 samples were found positive with the method MK/43 degrees C, and 27 with the methods MK/100ml/43 degrees C. The superiority of either one of the two R10 procedures over either one of the two MK methods is statistically highly significant (paired Chi2; P less than 0.001 in all four comparisons). The superiority of procedures MK/100 ml/43 degrees C over the method MK/43 degrees C is also statistically significant (P less than 0.005). Both R10/43 degrees C and R10/100 ml/43 degrees C procedures had a much stronger inhibitory effect on the competing organisms (lactose- and sucrose negative) than the two MK methods.

Animals↗

PubMed1979

[Antimeningococcal antibodies in healthy mothers and their newborn (author's transl)].

Blood specimen from 180 healthy mothers and their newborn were obtained during the delivery for the evaluation of placental transfer of anti-A, anti-B and anti-C antimeningococcal antibodies. The detection of titration of antibodies were performed by passive haemagglutination. Anti-A antibodies were found in 22%, anti-B in 64%, and anti-C in 30% of the mothers. In the newborns the corresponding proportions were 4.5%, 50% and 28%. For anti-A and anti-B antibodies a moderate positive correlation was found between the titre in mothers and their newborn. For anti-A antibodies, titres were generally low among both mothers and newborn and passive transfer was found in only 20%. For anti-B antibodies, titres were higher among both mothers and their newborn. Titre in newborn was as a rule lower than the corresponding titre of mother. Placental transfer of anti-B antibodies was high (78%). Finally, a strong positive correlation between the titre of mothers and their newborn was found for anti-C antibodies. Titres were usually similar in mothers and newborns and the placental transfer of anti-C antibodies was practically free (93%).

Antibodies, Bacterial↗

PubMed1979

[Sero-epidemiological characteristics of the MENINGOCOCCAL carrier state in Greece (author's transl)].

Nasopharyngeal swabs for the isolation and identification of meningococci, and blood specimens for the detection of antimeningococcal A, B and C antibodies were obtained from 341 Army and Navy recruits in the spring and autumn 1977. Samples were taken twice, at the arrival at the training camp and after 25 days. The main findings are as follows. 1) About 1/3 of the inductees at the arrival at the training camp were carriers of meningococci. Strains of serogroup B were more prevalent, followed by non-typable strains and strains of group C. After 25 days of residence in the camp the prevalence of carriers of meningococci has increased by 42%, and about half of the recruits were carriers. The prevalence of serogroup C has increased by 86%, of the non-typable strains by 48% and of serogroup B by 36%. 2) At the induction, 73% of the recruits had anti-B antibodies, whereas the corresponding figure was 33% for anti-A and 24% for anti-C. After 25 days in the camp the prevalence of anti-B antibodies was 83%, of anti-A 36% and of anti-C 47%. 3). At the induction the prevalence of meningococci B was higher among those who had no anti-B antibodies. Furthermore, the probability of a recruit who was not carrier of meningococcus B at the induction to become a carrier of meningococcus B at the second sampling was higher when he had no anti-B antibodies at the induction. Similar associations were not evident for serogroups A and C probably on account of the much smaller numbers in these groups. 4) The carrier state is associated with an increase of the titre of the corresponding antibodies.

Antibodies, Bacterial↗

PubMed1972