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C Sadakata

Publications and source records attributed to C Sadakata.

5 recordsLinked to original sources
PubMed1997

Flow-cytometric analysis on kainate-induced decrease in the cellular content of non-protein thiols in dissociated rat brain neurons.

In order to study the kainate-induced oxidative stress on brain neurons, the effect of kainate on cellular content of glutathione in rat cerebellar neurons were examined using a flow cytometer and 5-chloromethylfluorescein, a fluorescent dye for cellular non-protein thiols (mainly glutathione). Kainate at concentrations ranging from 30 microM to 1 mM produced a dose-dependent decrease in cellular content of glutathione. Exposure of neurons to kainate at concentrations of 300 microM or greater seemed to deplete cellular glutathione. Potency of kainate in reducing cellular content of glutathione was greater than those of glutamate and N-methyl-D-aspartate (NMDA). Kainate-induced decrease in cellular content of glutathione was partly attenuated by 6-nitro-7-cyano-quinoxaline-2,3-dione, a blocker of non-NMDA receptors and removal of external Ca2+. Results indicate that kainate causes Ca2(+)-dependent oxidative stress that decreases the cellular content of glutathione via activation of non-NMDA type of glutamate receptors.

Animals↗
PubMed1996

Oxidative stress-induced increase in intracellular Ca2+ and Ca(2+)-induced increase in oxidative stress: an experimental model using dissociated rat brain neurons.

In order to study the oxidative stress-induced change in intracellular concentration of Ca2+ ([Ca2+]i) and Ca(2+)-induced oxidative stress, effects of hydrogen peroxide and ionomycin, a calcium ionophore, on rat cerebellar neurons were examined using a flow cytometer and fluorescent dyes: fluo-3 for monitoring [Ca2+]i; 2',7'-dichlorofluorescin, for reactive oxygen species; and 5-chloromethylfluorescein, for cellular nonprotein thiols. Oxidative stress induced by hydrogen peroxide dose-dependently increased [Ca2+]i and decreased the content of nonprotein thiols. Ionomycin increased oxidative metabolism and decreased the content of nonprotein thiols. Results suggest that oxidative stress induces an increase in [Ca2+]i while an increase in [Ca2+]i increases oxidative stress in neurons.

Aniline Compounds↗