PubMed Health⌕ Search

Biomedical subjects

C Salerno

Publications and source records attributed to C Salerno.

95 records · Page 6Linked to original sources
PubMedDate not supplied

Expression of HLA class I antigens in human tumors and their involvement in tumor growth.

A decreased expression of major histocompatibility complex (MHC) class I antigens is a common feature of many experimental and human tumors and can often be correlated with malignancy grade. In fact, reduction of class I antigens is associated in most tumors with an enhanced ability to elude immune surveillance. Loss of HLA-A,B,C antigens ranges from a decrease in the percentage of A,B,C-positive cells to selective loss of particular antigens and total loss of class I molecule expression. In man, this has been documented in melanomas, carcinomas, lymphomas, neuroblastoma and acute leukemias. The reduction in membrane antigens is generally associated with a parallel fall in immunoprecipitable intracellular proteins and the corresponding mRNAs in the absence of structural changes in the coding genes. The literature concerning the above mentioned topics is reviewed and discussed.

Genes, MHC Class I↗
PubMedDate not supplied

[Effect of pesticides on Bacillus thuringiensis strains under controlled conditions].

Little is known about native populations of Bacillus thuringiensis (Bt) isolated from soils of Argentina. We undertook this study to determine the resistance to different pesticides of two commercial and fourteen native strains of Bt under in vitro conditions. An agar plate bioassay test conducted with ten pesticides and sixteen strains of Bt showed that Basagran, Scepter, Fungoxan and Decis were not toxic for the bioinsecticide bacteria at recommended application rates (RAR). In contrast, low concentrations (3.2% RAR) of Agil, Select and Isomero showed a deleterious effect on the bacteria investigated. Simultaneously, four of the pesticides were able to produce phenotypical changes on the Bt colonies grown on nutrient agar. Moreover, in a greenhouse experiment, seven pesticides applied at 1.6%, 12.5% and 100% RAR on soybean leaves were not as inhibitory as under in vitro conditions for two Bt strains (HD-1 and A61). However, survival of these strains in the phyllosphere of soybean differed significantly between untreated leaves and leaves treated with pesticides after 20 days of study (P < 0.05). Finally, and from an ecological point of view, these findings suggest that the addition of some pesticides to soybean leaves in lower concentrations than those recommended could be favourable for the persistence of Bt in this environment.

Bacillus thuringiensis↗
PubMedDate not supplied

Fluorescence polarization studies on the binding between glutamate dehydrogenase and cytoplasmic aspartate aminotransferase.

Polarization of fluorescence measurements on aspartate aminotransferase (from pig heart cytosol) labeled with fluorescein isothiocyanate have been used to detect the formation of a soluble complex of this protein wich glutamate dehydrogenase from bovine liver. The binding of the labeled transaminase to dehydrogenase is detectable at catalytic concentrations of the enzymes.

Animals↗