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Biomedical subjects

C Salmon

Publications and source records attributed to C Salmon.

At least 19 recordsLinked to original sources
PubMed1991

[Passive immunotherapy in AIDS: transfusion of plasma rich in anti-p25 antibody (phase I trial)].

The present study is a therapeutic trial of phase I, based on the principle of passive immunotherapy in acquired immunodeficiency syndrome (AIDS). Eighteen patients with full blown AIDS (stage IV C2 of CDC) were subdivided into two groups: nine receiving every two weeks 300 ml of plasma collected from HIV-1 seropositive symptomless (stage II or III of CDC) individuals, and nine (control group) receiving 300 ml of seronegative plasma at the same rythm and for the same period. Each patient received seven transfusions. Clinical and biological results during the transfusional and post-transfusional periods are reported.

Acquired Immunodeficiency Syndrome↗
PubMed1991

[DNA polymorphism: comparison of allelic frequencies obtained with two HVR probes in a population from Northeastern Brazil and in two other ethnic groups].

DNAS from 325 individuals representing 2 different populations (234 Brazilians and 91 Asiatics) were analysed for Restriction Fragment Length Polymorphisms (RFLPs). These DNAs were digested with Pvu II enzyme and successively hybridized to two HVR probes: alpha globin-3'HVR and Mucin-HVR. An allele frequency distribution was determined for each couple probe/enzyme and each ethnic groupe studied. The results were compared with frequencies observed in french population and we showed that there is no statistic significant difference between allele frequencies obtained with the couple probe/enzyme 3'HVR/Pvu II in Brazilian and French populations. We also showed that both populations studied (Brazilian and Asiatic) do not follow a Hardy-Weinberg balance.

Adolescent↗
PubMed1991

[Restriction fragment study (RFLP) of DNA polymorphism in criminology: quantitative and qualitative analyses beginning with dried blood and semen on various supports].

Deoxyribonucleic acid (DNA) purified from various forensic samples was characterized by RFLP (Restriction Fragments Length Polymorphism). DNA isolated from dried bloodstains and dried semen stains was digested with restriction endonucleases (HinfI, PvuII, TaqI) and fractionated by electrophoresis on 0.7% agarose gels. After transfer to a filter, DNA was hybridized with different radioactively labeled recombinant probes: with HinfI, G3, MS1, MS8, MS31, MS43, cloned from 33.6 and 33.15 Jeffreys probes, with PvuII 3'HVR, with TaqI MR24/1. This probes recognize polymorphic DNA regions, so variation between individuals at fundamental level of their DNA can be used to discriminate them. Therefore, pattern of RFLP detected by this test was used to determine the probable identity of different samples. DNA was isolated and detected by Southern blot from since 50 microliters dried bloodstains (HinfI, G3, MS1, MS31), from various specimen bloodstains and from dried semen stains. Highly variable minisatellites cloned from Jeffreys probes took more satisfaction for sensitivity than others. However, DNA polymorphism analysis can be used with caution because method limits can be perturbed interpretation of results.

Base Sequence↗
PubMed1991

[Purification and characterization of presumed thyrotropic hormone subunits of a teleost fish, the eel (Anguilla anguilla)].

We have only a partial knowledge of fish thyrotropins (TSH) and no data about peptide sequence are available. Various HPLC techniques allowed us to purify a 31.4 kDa, heterodimeric protein from saline eel pituitary extracts containing TSH activity. Partial N-terminal sequence (24 amino acids) from one subunit was strictly identical to that of eel gonadotropin II (GTH II) alpha subunit. With regard to the second subunit, 41% of the 22 amino acids identified at the N-terminus were homologous to those of bovine beta TSH and 36% were homologous to those of eel beta GTH II. Thus, the purified protein exhibits biochemical characteristics similar to those of mammalian TSH, in particular an alpha subunit common with GTH.

Amino Acid Sequence↗
PubMed1990

[Prediction of the clinical course of AIDS in a cohort of 77 seropositive persons. Importance of p24 antigen in the blood and the level of serum IgA].

Among the HIV-1 seropositive subjects detected through blood donation in Paris area who every six months voluntarily went through a thorough clinical and biological follow-up in the Institut National de Transfusion Sanguine, a cohort of 77 subjects had completed at least four biannual controls by september 1988. Upon inclusion in the study, all subjects were CDC stage II or III, none had received any treatment. Biological parameters in entrance in the study were assessed in a attempt to forecast the CD4 lymphocyte count decrease during the 18 months follow-up time. Multivariate forward stepwise analysis indicated that only p24 ag level, IgA level, and partially neopterin or Beta-2-Microglobulin levels were independently predictive of CD4 count at the end of the follow-up and of the CD4 loss at the same time. The prediction by these biological parameters is a rather poor one, 39% of the variation of CD4 lymphocyte count or 30% of the variation of CD4 loss after 18 months of the cohort follow-up being explained. In conclusion, HIV-infection seems to possess an intrinsic evolution which escapes our surveillance by biological parameters.

Acquired Immunodeficiency Syndrome↗
PubMed1990

Molecular cloning and protein structure of a human blood group Rh polypeptide.

cDNA clones encoding a human blood group Rh polypeptide were isolated from a human bone marrow cDNA library by using a polymerase chain reaction-amplified DNA fragment encoding the known common N-terminal region of the Rh proteins. The entire primary structure of the Rh polypeptide has been deduced from the nucleotide sequence of a 1384-base-pair-long cDNA clone. Translation of the open reading frame indicates that the Rh protein is composed of 417 amino acids, including the initiator methionine, which is removed in the mature protein, lacks a cleavable N-terminal sequence, and has no consensus site for potential N-glycosylation. The predicted molecular mass of the protein is 45,500, while that estimated for the Rh protein analyzed in NaDodSO4/polyacrylamide gels is in the range of 30,000-32,000. These findings suggest either that the hydrophobic Rh protein behaves abnormally on NaDodSO4 gels or that the Rh mRNA may encode a precursor protein, which is further matured by a proteolytic cleavage of the C-terminal region of the polypeptide. Hydropathy analysis and secondary structure predictions suggest the presence of 13 membrane-spanning domains, indicating that the Rh polypeptide is highly hydrophobic and deeply buried within the phospholipid bilayer. In RNA blot-hybridization (Northern) analysis, the Rh cDNA probe detects a major 1.7-kilobase and a minor 3.5-kilobase mRNA species in adult erythroblasts, fetal liver, and erythroid (K562, HEL) and megakaryocytic (MEG01) leukemic cell lines, but not in adult liver and kidney tissues or lymphoid (Jurkat) and promyelocytic (HL60) cell lines. These results suggest that the expression of the Rh gene(s) might be restricted to tissues or cell lines expressing erythroid characters.

Amino Acid Sequence↗
PubMed1979

[Lewis blood group activity in Fasciola hepatica].

Lewis blood group specificities are detected in the liver Fluke: Fasciola hepatica. Such specificities had never been reported in nature, other than in the human body. The investigation of this phenomenon might contribute to a better understanding of the mechanisms involved in parasite-host relationship.

Animals↗
PubMed1979

[K-Lymphocyte cytotoxicity: role of human blood group ABO, rhesus and P alloantibodies].

Using human erythrocytes of known antigenic density, sensitized by ABO and Rhesus (D) alloantibodies, it is shown that K cell cytotoxicity mediated by peripheral blood lymphocytes is directly correlated with the number of IgG molecules specifically bound to the target cell surface. The lytic sensitivity of P1k but not P2 or p erythrocytes coated with anti-Tja (anti-P + P1 + Pk) antibodies, demonstrates that effector K cells are mainly triggered through the IgG ANTI-Pk component of such sera.

ABO Blood-Group System↗
PubMed1979

[Association of acquired polyagglutinabilities of types T and B. An observation].

Although T and acquired B polyagglutinabilities are not exceptional, simultaneous occurence of the two types is a rarer phenomenon. Ten days after an open heart surgery operation, the patient, age 55, had an infectious syndrom : two strains, Clostridium perfringens and Peptococcus variabilis were isolated. Simultaneously, her red cells were found to be polyagglutinable. The association of T and acquired B polyagglutinabilities could be demonstrated by serological studies of the patient's red cells, and by in vitro transformation of normal red cells using culture supernatants obtained from the two isolated strains.

ABO Blood-Group System↗
PubMed1979

Detection of the H and I blood group antigens in normal plasma. A comparison with A and i antigens.

The levels of A, H, I and i antigens were measured in the plasma of 185 normal subjects by the agglutination-inhibition method. The presence of H in the plasma was only detectable with immune anti-H. The level of H in the plasma was directly correlated with the amount on the red cells, and was affected by the donor secretor status. The plasma of group O secretors contained more H than the plasma of donors of other phenotypes. On the other hand, I and i plasma antigens were not related to the other systems studied. Unlike the Ii antigens on the red cell membrane, there was no relationship between the levels of I and i in plasma.

ABO Blood-Group System↗
PubMed1979

Human IgE response to the administration of blood components. II. Repeated gammaglobulin injections.

43 adults from a renal dialysis unit staff have received regularly spaced gamma-globulin administrations for hepatitis B prophylaxis. Several blood samples were collected over a prolonged period of time (160 days). Following gamma-globulin administration, anti-immunoglobulin antibodies of the IgE class were detected in 80% of this population, a fortnight after the first injection using serum absorptions on polymerized gamma-globulins or a specific inverse RAST method. The reactivity pattern of these IgE anti-immunoglobulin antibodies was similar to that observed for the anti-immunoglobulin antibodies with "limited specificity" detected by passive hemagglutination, in that they reacted with only one of the immunoglobulins of the panel used for their detection. A decrease of the overall IgE levels was observed in 62% of the subjects for a prolonged period of time following gamma-globulin administration. This suggests a feedback regulation mechanism for the reagin production in man, as it has already been observed in animals. A high incidence of anti-immunoglobulin antibodies of various classes was observed in this study. However, only a small number (4/43) of adverse reactions appeared following gamma-globulin administration. For some of these subjects, the presence of specific IgE anti-immunoglobulin, detected by the inverse-RAST technique, suggests a possible role of such antibodies in some intolerance reactions to gamma-globulin administration.

Absorption↗