PubMed Health⌕ Search

Biomedical subjects

C Sanchez-Acedo

Publications and source records attributed to C Sanchez-Acedo.

6 recordsLinked to original sources
PubMed2001

A method for the sequential study of eimerian chromosomes by light and electron microscopy.

In the present study, the authors describe a simple method to isolate chromosomes from eimerian oocysts and to submit them to sequential study by light and electron microscopy. This method includes a reliable and reproducible technique for transferring eimerian chromosomes from slides to grid that fulfills the essential requirements for generalized use in cytogenetics. In addition, this method overcomes the difficulty of the resistance of protozoan oocysts to disruption and permits the release of intact meiotic chromosomes. The observation by the authors of synaptonemal complexes in meiotic chromosomes of different Eimeria species by applying the above-mentioned method to oocysts revealed its importance to future applications.

Animals↗
PubMed1998

Expression and localization of an S100 protein-like molecule in Eimeria tenella.

We investigated the expression of a calcium-binding protein, the S100 protein, in Eimeria tenella. Cecal paraffin sections from experimentally infected chickens were treated with anti-alpha-S100 (anti-alpha subunit of S100 protein) and anti-beta-S100 (anti-beta subunit of S100 protein) monoclonal antibodies and anti-S100 whole molecule polyclonal antibody. The avidin-biotin peroxidase method was used for immunocytochemical staining. Our results demonstrated a positive immunoreaction within the schizonts, macrogametes, and oocysts. These stages were all beta subunit and S100 whole-molecule positive. Immunoblot studies of the total proteins of E. tenella merozoites and sporozoites of the original strain and 2 precocious lines have demonstrated that precocious attenuation produced different S100 protein isotypes.

Animals↗
PubMed1996

Identification of a fibronectin-like molecule on the surface of Leishmania amastigotes.

The major surface glycoprotein of Leishmania gp63, a fibronectin-like molecule, plays a key role in parasite-macrophage interaction. In this article, we describe a cross-reactivity between an anti-fibronectin monoclonal antibody and the amastigote gp63 by means of immunoelectron microscopy and immunoblot. Immunoreactivity was found on the amastigote membrane and in the flagellar pocket. We suggest that gp63 may play a role in the protection and/or nutrition process of the parasite in the phagolysosomes of the macrophage.

Animals↗
PubMed1996

Identification of a fibronectin-like molecule on Eimeria tenella.

The attachment of Eimeria tenella to its target cells as an obligatory intracellular pathogen is essential for the development of disease. Previous reports have established that other intracellular protozoa parasites have either fibronectin, an adhesion protein, or fibronectin receptors, both of which are involved in the interaction with the host cells. In this current research, studies have been undertaken to visualize a surface component that may be involved in the attachment of E. tenella to host cells. For this purpose, monoclonal antibodies, both anti-chicken and anti-human fibronectin, and also anti-chicken integrin were used. Our results show a fibronectin-like molecule with an apparent molecular weight of 110 kDa in mature schizonts and microgametes. Staining with serum directed against chicken integrin revealed immunoreactivity within mature schizonts. Both the fibronectin-like molecule and the integrin may play an important role in the parasite stage-cell interaction and the promotion of parasite uptake.

Animals↗
PubMed1995

Detection of Blastocystis sp. in pigs in Aragon (Spain).

Infection by Blastocystis sp. has been detected for the first time in pigs in Spain. Detection was carried out by the ethyl acetate-formalin concentration method and direct microscopy. Vacuolated cells of Blastocystis sp. were found in stool samples from 27 of 360 pigs examined (7.5%). The protozoan was present on nine of 17 pig farms studied (53%). Infected pigs ranged from 1 month old to adults, but the organism was most frequent in 1-2 month old (18.4%) and 2-6 month old pigs (15.4%). No correlation was found between Blastocystis sp. infection and occurrence of diarrhoea in pigs.

Age Factors↗