Preparation of homogenous growth hormone from peritoneal dialysate.
Explore the source record for details and available documents.
Animals↗
Biomedical subjects
C Secchi
Publications and source records attributed to C Secchi.
[Action of some substrates on the oxygen consumption of the mucosa of the abomasum of Bos taurus].
Explore the source record for details and available documents.
Abomasum↗
A simplified procedure for purification of bovine growth hormone on Sephacryl S-200 superfine.
The isolation and purification of bovine growth hormone from pituitary glands by a shorter and efficient sequence of stages which utilizes the Reichert approach (Reichert, 1975) is described. The major innovations are: 1) precipitation in 2 M ammonium sulfate at pH 7.9 instead of pH 7.0 to obtain the extract designated as C-2 by Reichert and 2) early gel-chromatography on Sephacryl S-200 of the C-2 extract. The bGH obtained has higher specific activity than the standard reference hormone NIH-GH-B16. Electrophoretic and size homogeneity is investigated by isoelectric focusing and sodium dodecyl sulfate electrophoresis.
Acrylic Resins↗