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C Seidl

Publications and source records attributed to C Seidl.

59 records · Page 4Linked to original sources
PubMed1988

Evaluation of the biological effects of low-molecular-weight heparins.

In experiments with a laser thrombosis model in rat mesenteric venules, where laser beams induce defined injuries at the vessel endothelium, antithrombotic potency of low-molecular-weight (LMW) heparins was calculated by the number of laser injuries required to induce vessel occluding thrombi. The antithrombotic effect of various LMW heparins was measured at defined time intervals after subcutaneous application. Simultaneously, rat blood was collected and aXa and aIIa plasma levels were determined by chromogenic substrate assays. The antithrombotic effect calculated in the animal model lasted longer than 48 h after a single subcutaneous heparin injection whereas the anticoagulative effect measured as aXa or aIIa activity was measurable only within the first 6 h after subcutaneous application. LMW heparins showed an antithrombotic effect at dosages where no influence on the coagulation system could be demonstrated.

Animals↗
PubMed1988

The effects of two physical fitness programs designed for mentally retarded adults.

The purpose of this study was to design a systematic program of fitness training that enhances physical fitness of mentally retarded adults in sheltered workshops. The first phase of the study was a six-month program conducted by physical education graduate students while the second phase was four months in duration and led by workshop employees. Instructors followed a manual containing 48 lesson plans that were specifically prepared for mentally retarded participants. The physical fitness programs were evaluated using the Canadian Standardized Test of Fitness. MANOVA results revealed that both the six-month and four-month programs increased the physical fitness of the mentally retarded workers, although cardiovascular endurance improved only as a function of the four-month program.

Adult↗
PubMedDate not supplied

Analysis of glycoprotein Ia, Ib, IIb and IV RNA in platelets: quantitative determination using fluorescence-based polymerase chain reaction.

The aim of this study was to analyze the RNA level of glycoprotein (GP) receptors in platelets. We have therefore established a quantitative fluorescence-based polymerase chain reaction (PCR) to analyze GP Ia, Ib, IIb, and IV RNA. Isolation of platelet RNA was performed by guanidium isothiocyanate/phenol chloroform extraction. An internal standard consisting of cRNA copies from plasmid pAW109 was included before reverse transcription in each RNA sample and PCR amplification was performed using fluorescence-labeled primers. Subsequently, PCR fragments were separated by gel electrophoresis and quantitation of the GP-specific fragments was done by measuring the fluorescence intensities in comparison to the internal standard. Relative amount of GP RNA/platelet were calculated taking into account the number of platelets used for isolation of platelet RNA and the platelet size as determined by flow-cytometric analysis. Using this method we analyzed the GPIa, Ib, IIb and IV RNA content of platelets in healthy blood donors. In parallel experiments the number of GP cell surface receptors was measured by flow-cytometric analysis and correlated with the GP-specific RNA content. This method may be useful to study the GP-specific RNA content in platelets as well as in other tissues, such as megakaryocytes, especially in patients with congenital or acquired platelet function disorders.

Antigens, CD↗
PubMedDate not supplied

Value of quantitative DNA analysis in endocrine tumors of the pancreas.

BACKGROUND: The diagnosis of malignancy can be difficult in endocrine tumors of the pancreas. Moreover prognostically relevant factors are not available. The aim of this study was to evaluate retrospectively whether the DNA distribution pattern can differentiate between benign and malignant pancreatic endocrine tumors and secondly whether the DNA content of tumor cells gives prognostic information. METHOD: Image cytometry of paraffin-embedded tumor material of 42 pancreatic endocrine tumors. RESULTS: In 27 benign endocrine pancreatic tumors (25 insulinomas, 2 benign nonfunctioning endocrine tumors) we could differentiate between 6 diploid, 15 hypotriploid and 6 triploid DNA histograms. In 15 malignant endocrine tumors of the pancreas we could differentiate between 1 diploid, 1 hypotriploid, 9 triploid and 4 hypertriploid tumors. All 4 patients with a hypertriploid tumor died as a consequence of their disease in contrast to only 1 patient with a diploid, hypotriploid or triploid tumor even in case of a nonradical resection. CONCLUSION: With the help of quantitative DNA measurement a differentiation between malignant and benign endocrine pancreatic tumors is not possible even if the risk of malignancy increases with the DNA content. The DNA content of malignant endocrine pancreatic tumors has an influence on the long-term survival. Hypertriploid tumors have a statistically significantly worse prognosis than diploid, hypotriploid or triploid tumors.

Adenoma↗