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Biomedical subjects

C Sundström

Publications and source records attributed to C Sundström.

At least 127 records · Page 7Linked to original sources
PubMed1981

Lysis of human B-lymphocyte-derived lymphoma/leukemia cells of established cell lines by interferon-activated natural killer (NK) cells.

Cultured cell lines derived from malignant Epstein-Barr virus (EBV)-negative B cells, and representative of the more common types of naturally occurring non-Hodgkin lymphomas and related leukemias, were found to be sensitive to lysis by human natural killer (NK) cells. The observed lysis of such cell lines was mediated by a population of interferon-augmentable, FcR-positive, non-adherent lymphoid cells, which were also able to kill the "standard" NK targets K562 and Molt-4. When the NK susceptibility of the neoplastic, EBV-negative B cells was compared to that of diploid, EBV-carrying, non-neoplastic B lymphoblastoid cell lines (BLCLs) and of the "standard" NK target K562, several distinct patterns were observed. The killing of the neoplastic B cell lines was significantly less than that of K562, but significantly greater than that of the EBV-derived BLCLs of non-neoplastic origin. An additional finding was a similar NK susceptibility profile for the neoplastic, true histiocytic cell line U-937 (i.e., K562 greater than U937 greater than BLCLs). Furthermore, all cell lines, with the exception of the BLCLs, could effectively compete for the observed killing in cold target inhibition assays. The implications of these findings are discussed in relation to both neoplastic and non-neoplastic targets of NK lysis.

B-Lymphocytes↗
PubMed1981

Malignant thymus tumour progressing into acute leukaemia with non-specific esterase activity.

We describe an 8-year-old girl who presented a massive mediastinal tumour of thymic origin. Surgical excision was unsuccessful. Irradiation treatment (4,500 rad) together with intensive combination chemotherapy resulted in regression of the tumour. After three months, an increase of blasts was observed in the peripheral blood and bone marrow. The terminal phase was characterized by acute leukaemia, which did not respond to chemotherapy. Despite the thymic origin of this malignancy, the blast cells contained non-specific esterase activity as determined by naphthol-AS-acetate as substrate. The NaF-sensitive form of this enzyme is a characteristic of monocytes-macrophages. Other monocyte-macrophage markers were, however, negative. We conclude that the NaF-sensitive naphthol-AS-acetate esterase is not limited to tumours of the monocyte-macrophage system.

Bone Marrow Examination↗
PubMed1980

Phorbol ester-induced differentiation of chronic lymphocytic leukaemia cells.

The croton oil-derived tumour-promoting agent 12-O-tetra-decanoyl-phorbol-13-acetate (TPA) exerts pleiotropic effects on the differentiation and proliferation of both normal and malignant animal and human cells in vitro. TPA is mitogenic in nanomolar concentration to chickens embryo fibroblasts and human T lymphocytes and inhibits the terminal differentiation of various committed embryonic cells and mouse Friend erythroleukaemia or myeloid leukaemia cells. TPA induces a terminal cell differentiation in some murine and human myeloid leukaemia and histiocytic lymphoma cells. We report here the effect of TPA on chronic lymphocytic leukaemia (CLL) biopsy cells in vitro. In four out of five CLL patients studied, TPA induced the appearance of 90-100% of lymphoblastoid and plasmacytoid cells after 4 days of culture. Under the influence of TPA, 86-97% of the cells expressed with time increasing amounts of intracytoplasmic immunoglobulin (C-Ig) of the same phenotype as that detected on the surface (S-Ig) of fresh, non-induced CLL cells. A parallel decrease in both monoclonal S-Ig density and DNA synthesis of the CLL cells was observed. Electron microscopic studies showed a muturation towards plasma cells. We therefore conclude that TPA is capable of inducing differentiation of CLL cells in vitro.

Cell Differentiation↗
PubMed1980

An established human cell line derived from a malignant mediastinal teratoma.

An established cell line derived from a human malignant mediastinal teratoma is described. The cell line was composed of slightly atypical fibroblast cells with a population-doubling time of 24 h at the exponential growth phase. The reason why the emerging cell line became fibroblastic although other tissues were present in the original tumor may be that fibroblasts so readily grow in culture. Chromosome banding showed a hypertriploid stemline number, S = 78. Almost all of the analysed karyotypes had seven markers in common. Eleven other markers, occurring at low frequency, were also detected. The origin of all markers except one could be deduced.

Adult↗
PubMed1980

Liberation of a fibrogenic factor from human blood monocytes, ascites cells, cultured histiocytes and transformed mouse macrophages by treatment with SiO2.

Human monocytes and ascites macrophages from cirrhotic patients were isolated in Percoll-gradient and cultured with and without silica. Similar experiments were carried out also with cultured malignant human histiocytes and transformed mouse macrophages. The fibrogenic activity of the culture media was tested by measuring the incorporation of [3H]proline and [3H]thymidine into cultured rat granuloma and human synovial cells. Media from silica-treated monocytes, ascites macrophages and certain histiocyte and mouse macrophage lines caused an increase in the incorporation of both [3H]proline and [3H]thymidine into collagen and DNA, respectively, in both cell systems. Alkaline RNase activities were decreased markedly in the media from silica-treated ascites macrophages but not in the media of the monocytes or histiocytes.

Adult↗
PubMed1980

Mouse red cell receptors on human hematopoietic cell lines.

Spontaneous rosetting with mouse red blood cells (MRBC) seems to be a marker for a subpopulation of B-lymphocytes. We investigated the capacity of different hematopoietic cell lines to form rosettes with MRBC. The cell lines represent different hematopoietic malignancies and the results with these lines were compared with those obtained with non-malignant EBV carrying lymphoblastoid cell lines (LCL). Among tumor cell lines only exceptional lines, 1 myeloma and 2 leukemia lines, showed rosetting capacity with MRBC. Most LCL, however, bound MRBC. These results indicate that only 1 category of established hematopoietic cell line, the LCL, has MRBC receptors and that thus a further subset of B-lymphocyte, the one represented by LCL, has the capacity for rosetting with MRBC. This MRBC rosetting of LCL is, however, not restricted to those lines with S-IgM. Tumor lines of hematopoietic origin seem, however, in general to lack MRBC receptors, even such lines that are derived from malignant lymphomas which express other B-lymphocyte characteristics.

Animals↗
PubMed1979

Pernicious anaemia in association with argyrophil (Sevier-Munger) gastric carcinoid.

3 cases of pernicious anaemia in association with gastric carcinoid (in 1 case multiple) are described. All tumours were rich in argyrophil cells with the Sevier-Munger and Grimelius staining techniques, indicating that they might be of the enterochromaffin-like cell type. The possible causal relationship between pernicious anaemia and this type of argyrophil carcinoid is discussed.

Aged↗
PubMed1979

Clinical and surgical staging of Hodgkin's disease.

Staging laparotomy was performed in 36 patients with Hodgkin's disease. The surgical procedure changed the stage in 14 cases or 36% and also revealed subdiaphragmatic disease in 14 patients. Mixed cellularity and nodular sclerosis were the main histopathological types, contributing 44% and 42%, respectively, of the whole material. Postoperative complications occurred in 8 cases (22%)--none was fatal. The average hospital stay was 9.5 days, the longest being 22 days.

Adolescent↗
PubMed1978

Presence of alpha-naphthyl acetate esterase activity in human haematopoietic cell lines and in fresh biopsy specimens of lymphoma and myeloma.

We have previously shown that non-proliferating human T- but not B-lymphocytes contain demonstrable amounts of acid alpha-naphthyl acetate esterase (ANAE). The usefulness of this histochemical marker for the diagnosis and classification of malignant lymphoid tumors was investigated by use of a panel of established normal and malignant human haematopoietic cell lines and fresh biopsy cells from malignant lymphomas and myelomas. The results showed that not only the T-cell derived acute leukaemia lines, but also histiocytic lymphoma and myeloma lines and some of the lymphoma (Burkitt and lymphocytic) and non-neoplastic lymphoblastoid cell lines with B-cell surface markers expressed strong ANAE reactivity. Some but not all of the immunoglobulin producing myeloma and lymphocytic lymphoma biopsies were ANAE-positive. Inhibition experiments with sodium fluoride and E-600 demonstrated that although the T-lymphocyte specific esterase is predominantly of 'A'-type, the malignant lines contain also non-specific 'B' esterase and pseudocholinesterase. As the presence of the various esterases did not demonstrate any specific distribution pattern among he haematopoietic cell lines of different origin, we concluded that the ANAE marker is no longer T-specific when malignant lymphoid cells are considered, and that the usefulness of this marker in routine diagnostic work therefore is limited.

B-Lymphocytes↗
PubMed1978

Human malignant lymphomas in vitro. Characterization of biopsy cells and establishment of permanent cell lines.

A series of 55 biopsies from different types of malignant lymphomas were characterized in short-term culture experiments and during prolonged growth in vitro. The majority of the lymphocytic lymphomas and half of the histiocytic lymphomas expressed surface immunoglobulin, either in monoclonal or polyclonal form, indicating B-lymphocyte derivation. No lysozyme production was noted in either type of lymphoma, giving further support to the notion that histiocytic lymphomas are not truly histiocytic. Production of beta2-microglobulin was higher in histiocytic than in lymphocytic lymphoma and Hodgkin's disease but did not significantly differ from the production observed in non-neoplastic lymph node disorders. Incorporation of 3H-thymidine varied greatly within each category of lymphoma; the highest mean labelling index was noted in histiocytic lymphoma, possibly reflecting the generally more malignant course in such cases. Epstein-Barr virus-associated nuclear antigen was observed in one case of Hodgkin's disease. Attempts to establish permanent tumor cell lines were successful only from two explants of lymphocytic lymphoma and one pleural effusion from histiocytic lymphoma. The two cell lines derived from lymphocytic lymphomas both exhibited B-lymphocyte characteristics. The histiocytic lymphoma line lacked lymphocyte markers, produced lysozyme and was found to be rich in cytoplasmic esterases. These features are consistent with a "true" histiocytic derivation of this line. Lymphoblastoid cell lines representing non-neoplastic EBV-carrying lymphocytes contaminating the biopsies were derived from 19 biopsies, with the highest frequency noted in cultures of biopsies from Hodgkin's disease. The tumor lines were all EBV-genome negative.

Antigens, Viral↗
PubMed1977

Cytochemical profile of human haematopoietic biopsy cells and derived cell lines.

Twenty-three human haematopoietic cell lines, normal and mitogen stimulated peripheral blood lymphocytes and tumour material from fresh leukaemias, myelomas and lymphomas were investigated with a panel of cytochemical reactions. Normal and mitogen stimulated lymphocytes, non-neoplastic lymphoblastoid cell lines (LCL), lymphoma lines with B-lymphocyte characteristics, chronic lymphocytic leukaemia and fresh lymphocytic lymphomas reacted weakly or negatively with all stains. T-lymphocyte acute leukaemia lines were PAS and alpha-naphtyl acetate esterase positive. Myeloma lines and fresh myelomas were strongly beta-glucoronidase positive. A histiocytic lymphoma cell line was strongly esterase positive with naphtol AS-D acetate esterase inhibited by NaF. The three fresh histiocytic lymphomas, however, reacted as the lymphocytic lymphomas suggesting a lymphoid origin. A myeloid leukaemia line was strongly positive for acid phsophatase. No major disagreement was noted between the reactivity of established neoplastic lines and the corresponding fresh biopsy cells indicating an unaltered qualitative expression of enzyme production after prolonged in vitro culture.

Cell Line↗
PubMed1977

Pneumatosis cystoides intestinalis. Report of two cases.

The histories of two patients with pneumatosis cystoides intestinalis are reported. In the first case a left hemicolectomy was performed because of suspicion of polyposis coli. The other patients was given symptomatic treatment. Conservative treatment is recommended by most authors, but since the disorder is seldom seen, unnecessary surgery is often performed because of incorrect preoperative diagnosis.

Aged↗
PubMed1976

Establishment and characterization of a human histiocytic lymphoma cell line (U-937).

A human hematopoietic cell line (U-937) with exceptional characteristics was derived from a patient with generalized histiocytic lymphoma. The morphology of the cell line was identical to that of the tumor cells in the pleural effusion from which the line was derived. Since Epstein-Barr virus (EBV) carrying diploid lymphoblastoid cell lines unrelated to the tumor population often become established in vitro from non-Burkitt lymphoma explants, several parameters were studied to discriminate the U-937 from such lines: morphology in vitro, growth characteristics, cytochemistry, surface receptor pattern, Ig production, lysozyme production, beta2-microglobulin production, presence of EBV genome and karyotype. In all these respects U-937 differed from prototype lymphoblastoid cell lines. The histiocytic origin of the cell line was shown by its capacity for lysozyme production and the strong esterase activity (naphtol AS-D acetate esterase inhibited by NaF) of the cells. It is therefore concluded that the U-937 is a neoplastic, histiocytic cell line.

Adult↗
PubMed1976

A highly differentiated exocrine pancreatic tumour in a young boy.

A primary pancreatic tumour in a 6-year-old boy was removed by radical excision. From both light and electron microscopic observations the tumour was considered to be of exocrine origin, with some differentiation towards acinar cells. Although the tumour was highly differentiated, it was regarded as probably malignant because of tumour growth into the surrounding fibrous capsule. The case is discussed in relation to earlier rare reports of pancreatic tumours in infants and children.

Child↗
PubMed1976

Thymoma in a case of scleroderma.

A case of scleroderma with manifestations in the oesophagus and both lungs and a concomitant thymic lympho-epithelioma is presented. This case represents a further documentation of the well-established association between thymoma and collagen disorders.

Aged↗