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C Svanborg-Edén

Publications and source records attributed to C Svanborg-Edén.

49 records · Page 3Linked to original sources
PubMed1982

Different binding specificities of P. mirabilis compared to E. coli.

The target cell specificities of P. mirabilis and E. coli strains were compared using human urinary tract epithelial cells and erythrocytes from human, guinea pig, sheep, ox and horse blood. P. mirabilis strains agglutinated the erythrocytes both in mannose-resistant and mannose-sensitive patterns. No relation was, however, found between hemagglutination and adhesion to uroepithelial cells. This is in contrast to E. coli where uroepithelial cell attachment is paralleled by mannose-resistant agglutination o human erythrocytes. The difference in receptor recognition between P, mirabilis and E. coli was further indicated by the fact that P. mirabilis strains attaching to human uroepithelial cells did not agglutinate guinea pig red cells coated with globotetraosylceramide, acting as a receptor for E. coli on human uroepithelial cells erythrocytes.

Animals↗
PubMed1981

Glycolipid receptors for uropathogenic Escherichia coli on human erythrocytes and uroepithelial cells.

A specific family of glycolipids, the globoseries, was shown to act as receptors on human uroepithelial cells and erythrocytes for the majority of uropathogenic Escherichia coli strains attaching to or hemagglutinating those cells. This was demonstrated in three different ways: (i) correlation between the natural presence of glycolipid in the target cell (erythrocytes of different species) and binding of bacteria; (ii) inhibition of attachment to human uroepithelial cells by preincubation of bacteria and glycolipid; and (iii) induction of binding to unreactive cells by coating of these cells with glycolipid. Strains reacting with the receptor agglutinated guinea pig erythrocytes in a mannose-resistant way after, but not before, coating of the cells with globotetraosylceramide. Unrelated glycolipids were not recognized. The reaction was made independent of simultaneous occurrence of mannose-sensitive adhesions on the strains by addition of D-mannose. The receptor-coated cells were used as a tool to screen for prevalence of receptor recognition in a collection of 453 E. coli strains isolated from patients with urinary tract infection or from the stools of healthy children. Of 150 strains attaching to human uroepithelial cells and agglutinating human erythrocytes, 121 bound to globotetraosylceramide (81%). Globoside recognition was especially frequent among pyelonephritis strains (74/81). The glycolipid composition of the urogenital epithelium and kidney tissue and the ability of uropathogenic E. coli to bind to these glycolipids may be a determinant in host-parasite interaction leading to urinary tract infection.

Animals↗
PubMed1979

Effects of subinhibitory amounts of ampicillin, amoxycillin and mecillinam on the adhesion of Escherichia coli bacteria to human urinary tract epithelial cells: a preliminary study.

Attachment to mucous surfaces may be a prerequisite for bacteria colonizing these surfaces or invading underlying tissues. Subinhibitory amounts of ampicillin and amoxycillin but not mecillinam decreased the attachment of Escherichia coli bacteria to human uro-epithelial cells in vitro. No significant synergistic effect on the attachment by the antibiotics was obtained. The present report indicates a new parameter for the study of antibacterial actions of drugs.

Adhesiveness↗
PubMed1979

Attachment of Escherichia coli to urinary sediment epithelial cells from urinary tract infection-prone and healthy children.

Escherichia coli isolated from patients with recurrent urinary tract infections were tested for ability to attach to human urinary sediment epithelial cells in vitro. Higher mean capacity to bind bacteria was found for epithelial cells of the patient from whom the E. coli strain had been isolated than for epithelial cells from subjects without a history of urinary tract infection. The two populations were age matched. No relation was found between the age of the cell donor (0 to 15 years) and the capacity for E. coli attachement.

Adolescent↗
PubMed1978

Secretory immunoglobulin A and G antibodies prevent adhesion of Escherichia coli to human urinary tract epithelial cells.

The adhesion of Escherichia coli to human urinary tract epithelial cells was inhibited by commercial gamma globulin, the total immunoglobulin fraction of human breast milk and urine, as well as the isolated immunoglobulin G and secretory immunoglobulin A fractions of urine from patients with acute pyelonephritis. Urinary anti-O6 antibodies reduced the adhesion of several O6 strains. Absorption of antibodies to the lipopolysaccharide of the adhering strain markedly decreased the antiadhesive capacity of all the immunoglobulin preparations, whereas elimination of antibodies to the capsular polysaccharide antigen consistently had a small but not significant effect. When urine was absorbed with whole, live bacteria of the patients' infecting strains, the antiadhesive effect completely disappeared. Absorption with bacteria lacking pili only partially reduced this effect.

Antibodies, Bacterial↗
PubMed1978

High frequency of K15 antigen in enterotoxigenic Escherichia coli: lack of correlation to adherence to epithelial cells or sensitivity to bactericidal effect of normal serum.

Typing of E. coli O and K antigens, testing of sensitivity to bactericidal effect of normal serum and adherence to uroepithelial as well as buccal epithelial cells was performed in enterotoxinogenic (ETEC) and normal E. coli strains. The 99 ETEC faecal strains were obtained from Swedish and Ethiopian children and adults and Swedish soldiers in Cyprus with diarrhoea. The reference faecal strains were isolated from 120 healthy Swedish children. E. coli K15 was significantly more often seen in ETEC strains than in the reference strains, always in combination with O6 antigen. K15 is hardly ever seen in nonenterotoxinogenic strains. There was no significant difference in sensitivity to bactericidal effect of serum or adherence to epithelial cells between ETEC strains and normal strains. The epidemiological significance of the findings is discussed.

Adult↗
PubMed1977

Antigens of Escherichia coli, human immune response, and the pathogenesis of urinary tract infections.

Acute pyelonephritis (but not cystitis or "asymptomatic" bacteriuria) due to Escherichia coli induces serum antibodies to O-but rarely to K-antigens, especially not to the most common antigen, K1. Locally produced secretory IgA and IgG antibodies to O-and K-antigens appear in urine during most infections. The E. coli in urine of patients with asymptomatic bacteriuria are different from those in patients with acute pyelonephritis and cystitis and undergo continuous changes, presumably caused by the local antibody response. The E. coli become less virulent and are less able to attach to uroepithelial cells than E. coli causing acute symptomatic infections. Antibodies in urine prevent epithelial adherence. Parenteral and intravesicular injections of killed bacteria can protect against ascending pyelonephritis in rats. A few K-antigens dominate among E. coli that cause urinary tract infections. Vaccination of problem cases is a possibility because of the protective nature of K-antibodies. The mechanism of renal scarring that appears in some patients with urinary tract infections is unknown. Autoantibodies to the Tamm-Horsfall protein that increase after acute pyelonephritis or the cross-reactions noted between certain E. coli and antigens on the kidney may be involved.

Animals↗
PubMedDate not supplied

Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis.

Thirty-two Escherichia coli strains from 30 children with pyelonephritis were examined for their haemagglutination patterns and O and K serotypes. 29 (91%) of the strains showed mannose-resistant haemagglutination (MRHA). By use of well-defined target cells, these MRHA+ strains could be shown to recognise human cells either in a P-specific manner (recognising a specific galactosyl-galactose structure which is part of P blood groups antigens) or in a separate, X-specific manner. Both recognition mechanisms could occur separately or together on the same bacteria, the frequencies of P and X specificity being 81 and 19%, respectively. Both MRHA and P specificity were significantly associated with the O antigens 01, 04, 06, 016, and 018, and the capsular antigen K1, which have previously been associated with pyelonephritis. However, the association of MRHA and P specificity with upper urinary tract infection in children is greater than that of any other laboratory-defined bacterial characteristic.

Antigens, Bacterial↗
PubMedDate not supplied

Effects of subminimal inhibitory concentrations of ampicillin, chloramphenicol, and nitrofurantoin on the attachment of Escherichia coli to human uroepithelial cells in vitro.

An in vitro system with epithelial cells of the human urinary tract and Escherichia coli isolated from the urine of pateints with acute pyelonephritis was used for tests of the effect of antibacterial agents on bacterial adhesion. E. coli treated with subminimal inhibitory amounts of ampicillin attached less than untreated control bacteria. Subminimal inhibitory amounts of chloramphenicol and nitrofurantoin had no effect on adhesion of the 15 strains of E. coli tested. The combination of bacterial pretreatment with ampicillin and addition of antibodies to the adhesion test tube decreased adhesion more than did either treatment separately.

Adhesiveness↗
PubMedDate not supplied

Attachment of Streptococcus pneumoniae to human pharyngeal epithelial cells in vitro.

The aim of this study has been to develop a test system for attachment of pneumococci to pharyngeal cells. Cells scraped from a healthy person were washed and quantitated, bacteria added and after incubation the suspension was washed and the number of adhering bacteria counted. Among strains from different infectious foci the highest number of strains with adhesive capacity was found in the otitis media group. Capacity to attach to the pharyngeal mucosa might be a virulence factor of pneumococci causing otitis media. There was a lack of distinct relation between capsular serotype and adhesive capacity indicating that the polysaccharide is not primarily responsible for the binding to epithelial cells. If attachment to the pharyngeal mucosa is a first step necessary for colonization and subsequent invasion of the tissues, then antibiotics in the secretions preventing such colonization might be of protective value in otitis-prone children.

Adhesiveness↗