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C T Dolan

Publications and source records attributed to C T Dolan.

At least 19 recordsLinked to original sources
PubMed1982

Performance of yeast identification systems. An analysis of the College of American Pathologists Special Mycology Survey data.

The performance of approximately 400 participants in the CAP Special Mycology Survey in identifying 15 medically important yeasts was analyzed. With nine strains, users of the API 20C system and Uni-Yeast-Tek (UYT) systems performed comparably. With two strains (Trichosporon cutaneum and Candida guilliermondii) the UYT users performed significantly better than API users; with four strains (Cryptococcus albidus, Saccharomyces cerevisiae, and two strains of Candida tropicalis), API users significantly outperformed UYT users. Analysis of biochemical reaction patterns demonstrated, with a few exceptions, good reproducibility among laboratories with both kits. With one exception (melezitose-variable Candida albicans), the few discrepancies in biochemical reactions had little or no influence on the final identification. From this analysis, we conclude that both API and UYT perform acceptably well in routine clinical laboratories when used in conjunction with morphologic observations and, when indicated, supplemented biochemical test.

Evaluation Studies as Topic↗
PubMed1981

Summary and analysis of the mycology proficiency testing survey results of the College of American Pathologists. 1976-1979.

The results of the CAP Mycology Proficiency Testing Survey Programs for 1976 through 1979 are summarized and analyzed. There has been a slight, but definite, improvement in participant performance over this four year period and considerable improvement over the performance of 1972. The problems in analyzing participant performance are reviewed. The Survey Program itself is examined in the light of the stated objectives, and areas for improvement noted.

Aspergillus flavus↗
PubMed1971

Optimal combination and concentration of antibiotics in media for isolation of pathogenic fungi and Nocardia asteroides.

Strains of Blastomyces dermatitidis, Sporothrix schenckii, Histoplasma capsulatum, Cryptococcus neoformans, Nocardia asteroides, and Coccidioides immitis were tested for in vitro susceptibility to polymyxin, gentamicin, kanamycin, chloramphenicol, and neomycin at concentrations of 1, 2, 4, 8, and 16 mug/ml. Polymyxin was the most inhibitory and gentamicin was the least inhibitory of the five antibiotics. Two Histoplasma mycelial strains were partially inhibited by 2 and 8 mug of gentamicin per ml and showed at least a 2+ growth at the higher antibiotic concentration. Kanamycin and neomycin produced significant inhibition of N. asteroides but otherwise were noninhibitory. A combination of chloramphenicol and kanamycin, each at 16 mug/ml, and gentamicin, at 4 mug/ml, was noninhibitory to the strains tested except for N. asteroides. Chloramphenicol at 16 mug/ml was not inhibitory for N. asteroides. The results suggest that the optimal antibiotic combination to use in the isolation of fungi and higher bacteria is chloramphenicol, 16 mug/ml, and gentamicin, 4 mug/ml. Addition of sheep blood (5%) had no effect on antibiotic susceptibility of the organisms studied.

Agar↗