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Biomedical subjects

C Takada

Publications and source records attributed to C Takada.

At least 19 recordsLinked to original sources
PubMed2004

Effects of olopatadine hydrochloride, an antihistamine drug, on skin inflammation induced by repeated topical application of oxazolone in mice.

BACKGROUND: Olopatadine hydrochloride (olopatadine) is one of the second-generation antihistamines, which is prescribed for allergic disorders such as rhinitis, urticaria and eczema dermatitis. OBJECTIVES: To investigate the possible anti-inflammatory effect of olopatadine on the chronic contact hypersensitivity response to repeated topical application of oxazolone in mice. METHODS: The preventive and therapeutic effects of oral olopatadine were quantified by measurements of ear swelling, cytokine protein and mRNA expression in the ear lesion, and were compared with those of topical betamethasone 17-valerate (betamethasone). RESULTS: The ear receiving repeated applications of oxazolone exhibited erythema, oedema and abrasion. Both preventive and therapeutic administration of olopatadine (10 mg kg(-1) day(-1)) significantly inhibited the ear swelling and the increased production of interleukin (IL)-4, IL-1beta, granulocyte-macrophage colony-stimulating factor (GM-CSF) and nerve growth factor. In the histopathological analysis, olopatadine ameliorated epidermal hyperplasia and infiltration of inflammatory cells. Consistent with these results, olopatadine significantly reduced the increased expression of interferon-gamma and IL-4 mRNA. Although betamethasone (0.012 mg ear(-1) day(-1)) showed similar activities to olopatadine against these responses, it caused atrophy of the ear skin. CONCLUSIONS: These results indicate that olopatadine is an antihistamine agent having inhibitory activities against chronic inflammatory dermatitis, possibly resulting from its diminishing effect on elevated cytokines.

Adjuvants, Immunologic↗
PubMed2002

Endosonographic diagnosis of pneumatosis cystoides intestinalis in infancy.

We report pneumatosis cystoides intestinalis in a 10-month-old girl who developed bloody diarrhea following chemotherapy for leukemia. The diagnosis was made only by colonic endoscopic ultrasonography, whereas the abdominal plain radiogram and computed tomography failed to elucidate the diagnosis. She was successfully treated with hyperbaric oxygen therapy. Wider application of endoscopic ultrasonography may lead to the more frequent detection of pneumatosis cystoides intestinalis, currently a rare disorder.

Endosonography↗
PubMed2001

Cloning, sequencing, and expression of the gene encoding an intracellular beta-D-xylosidase from Streptomyces thermoviolaceus OPC-520.

The intracellular beta-xylosidase was induced when Streptomyces thermoviolaceus OPC-520 was grown at 50 degrees C in a minimal medium containing xylan or xylooligosaccharides. The 82-kDa protein with beta-xylosidase activity was partially purified and its N-terminal amino acid sequence was analyzed. The gene encoding the enzyme was cloned, sequenced, and expressed in Escherichia coli. The bxlA gene consists of a 2,100-bp open reading frame encoding 770 amino acids. The deduced amino acid sequence of the bxlA gene product had significant similarity with beta-xylosidases classified into family 3 of glycosyl hydrolases. The bxlA gene was expressed in E. coli, and the recombinant protein was purified to homogeneity. The enzyme was a monomer with a molecular mass of 82 kDa. The purified enzyme showed hydrolytic activity towards only p-nitrophenyl-beta-D-xylopyranoside among the synthetic glycosides tested. Thin-layer chromatography analysis showed that the enzyme is an exo-type enzyme that hydrolyze xylooligosaccharides, but had no activity toward xylan. High activity against pNPX occurred in the pH range 6.0-7.0 and temperature range 40-50 degrees C.

Amino Acid Sequence↗
PubMed1999

Participation of the pyrazine cation radical in the formation of mutagens in the reaction of glucose/glycine/creatinine.

Two classes of the free radical Maillard intermediates, the pyrazine cation radical and the carbon-centered radicals, are detected in the reaction of Glc (glucose)/Gly (glycine) by electron spin resonance and spin trapping technique. Profile of the generation of the pyrazine cation radical in the reaction with different ratios of the reactants was found to be similar to that of the formation of mutagens in the subsequent reaction with creatinine. By contrast, profile of the generation of the carbon-centered radicals was not consistent with that of the mutagen formation. Thiol antioxidants and unsaturated fatty acids (or their esters) effectively scavenged the pyrazine cation radical generated in the reaction of Glc/Gly, and inhibited the formation of the mutagens in the reaction of Glc/Gly and creatinine. Ethanol, a sulfide and a saturated fatty acid were not effective to scavenge the pyrazine cation radical and did not inhibit the mutagen formation. The pyrazine cation radical rather than the carbon-centered radicals may play an important role in the mutagen formation. Thiol antioxidants and unsaturated fatty acids can be evaluated as inhibitors of the pyrazine cation radical-derived formation of the mutagens.

Cations↗
PubMed1994

[A case report of toxic shock-like syndrome associated with prevalence of streptococcal pharingitis in the family].

A case of streptococcal toxic shock-like syndrome in a previously healthy, 57 year old Japanese female has been reported. Initially, she had a sore throat and low grade fever for 5 days. Because of sudden severe pain on the extremities and erythema on bilateral forearms, she was hospitalized. On admission, her conciousness was clear. Although profound hypotension, anuria and prolonged blood coagulation were observed. Antibiotics, fluid therapy and dopamine were given. Four hours after admission, she died in spite of resuscitation efforts, by sudden cardiac arrest. Streptococcus pyogenes was isolated in her blood. At the same time as when she died, three of the five people of the patient's family living with her, had pharingitis or pneumonia. From the pharynxs of the three people with pharingitis, Streptococcus pyogenes was also isolated. The serotype of all organisms was T11, and they produced exotoxintype B in vitro. This case suggests that infection of Streptococcus pyogenes is not essential for the development of toxic shock-like syndrome.

Family Health↗
PubMed1993

[Infusion therapy for acute superior mesenteric artery occlusion].

We performed transcatheter fibrolytic therapy using a coaxial microcatheter in two cases of acute superior mesenteric artery occlusion in the early stage. The tip of the microcatheter was introduced into the clot of the superior mesenteric artery. Urokinase was infused at a rate of 10,000 IU/min. The clots dissolved immediately after the infusion of 240,000 IU and 480,000 IU, respectively, without any complications. This infusion technique is promising for the treatment of acute superior mesenteric artery occlusion.

Aged↗
PubMed1991

[Result of surgical treatment in 226 cases of primary lung cancer].

From August, 1979 to June, 1990, 226 cases of primary lung cancer were treated surgically at our Department. Of these, 176 cases, excluding hospital deaths from lung cancer and other diseases, were analyzed according to prognosis. The 5-year survival rate of the 176 cases was 40.2% and the 5-year survival rates according to sex were, male, 36.1%; female, 53.2%. The 5-year survival rates according to postoperative stage were, stage I, 64.1%; stage II, 44.1%; stage III A, 22.1%; stage III B & IV, 0% and the 5-year survival rates according to histologic type were, adenocarcinoma, 40.5%; squamous cell carcinoma, 49.7%; large cell carcinoma, 18.9%; small cell carcinoma, 0%. The 5-year survival rates according to primary site were, right side, 43.8%; left side, 34.6%; right upper lobe, 39.7%; right middle lobe, 60.0%; right lower lobe, 44.4%; left upper lobe, 41.7%; left lower lobe, 18.1%. The prognosis of squamous cell carcinoma, and the early stage, female group, right side group, right middle & lower lobe group, were all fairly good.

Adenocarcinoma↗
PubMed1990

Radiation therapy of invasive thymoma.

Between 1978 and 1987, 30 cases of invasive thymomas were treated with radiotherapy after surgery. Surgical therapy consisted of total resection in 15 patients, subtotal resection in 1 patient, and biopsy in 14 patients. Myasthenia gravis (MG) was associated in nine patients (MG(+) group), but in 21 patients there was no evidence of myasthenia gravis (MG(-) group). Irradiation in the dose range of 30 to 58.7 Gy was delivered. The total average 5-year survival rate was 71.8%; it was 39.2% in MG(+) group and 78.3% in MG(-) group, though there was no significant statistical difference. Myasthenia gravis was well controlled by the tumorectomy and associated radiotherapy in 7 of the 9 patients. However, in 3 of 7 patients (42.9%) myasthenia gravis recurred at 2 years, 2 years and 7 months, and 5 years and 8 months after initial therapy. Total body irradiation of 2 Gy with 0.1 Gy fractions was administered for uncontrollable myasthenia gravis in one patient with marked improvement. Radiation therapy is an important therapeutic modality for unresectable malignant thymoma as well as for postoperative combined therapy. Total body irradiation may be an effective method to treat patients with otherwise resistant myasthenia gravis.

Adolescent↗
PubMed1988

[A case of recurrent epipharyngeal cancer showing complete disappearance following suppository administration of tegafur (Sunfural rectal capsules)].

A 55-year-old woman was diagnosed as having epipharyngeal cancer, characterized by T2N1M0 and poorly differentiated squamous cell carcinoma. She was treated by radiotherapy (primary site: 65 Gy, neck: 75 Gy) and the tumor completely disappeared. However, 4 months later, the tumor recurred in the epipharynx. Tegafur in the form of a suppository (Sunfural Rectal Capsules, 750 mg) was given once a day. During the course of epipharyngoscopic follow-up the tumor almost totally disappeared at a dose of 10,250 mg (15 days). Biopsy failed to reveal any cancer cells and computed tomography showed no tumor masses in the epipharynx. At present, 6 months have already passed since the start of Tegafur administration, there is no recurrence of the cancer, and the chemotherapy is being continued at our outpatient clinic. By the standard for the efficacy of cancer chemotherapy proposed by Koyama and Saito, this case corresponds to a state of complete response.

Carcinoma, Squamous Cell↗
PubMed1987

Radiotherapy of spontaneous carotid-cavernous sinus fistulas.

Between 1984 and 1986, 7 patients with spontaneous carotid-cavernous fistulas (CCF) were treated by radiotherapy delivering 3,000 cGy (200 cGy, 5 times per week) to the sellar region. Improvements of clinical signs and symptoms were seen in all patients within 6 months of treatment. During a follow-up period of 7 to 35 months, 6 patients remained in good clinical condition and only one patient developed a recurrence. As an adverse effect, one patient developed early menopause, but no other side-effects or complications were seen. Radiotherapy should be considered in patients with spontaneous CCF, when CCF are seen in middle-high aged patients and progressive without relief of symptoms.

Adult↗