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C Thierry

Publications and source records attributed to C Thierry.

32 records · Page 2Linked to original sources
PubMed1979

Human autologous rosettes. II. Prognostic significance of variations in autologous rosette-forming cells in the peripheral blood of cancer patients.

The distribution of autologous rosette-forming cells (A-RFC) was determined in 55 samples of peripheral blood mononuclear cells from healthy adult donors and 407 samples from 112 cancer patients. The mean percentage and absolute number of A-RFC were significantly reduced in the cancer patients compared to the healthy adult donors. These values for A-RFC were even more significantly reduced in patients with cancer relapse than in patients with complete remission of cancer. Thus diminution of this pertinent subset of T-lymphocytes has interesting clinical value by its being closely related to an adverse prognosis.

Adolescent↗
PubMed1979

Thymosin modulation of suppressor function in mice and man.

The effect of thymosin on suppressor-cell function was evaluated in vivo in a murine tumor system and in vitro on human lymphocytes. In mice, the Lewis tumor system was used. We showed that splenocytes from tumor-bearing animals were able to enhance tumor growth in a syngeneic system. This enhancement was similar when thymocytes from tumor-bearing animals were used and disappeared after anti-Thy 1-2 antiserum treatment, suggesting a T-dependence. Treatment of the tumor-growth-enhancing lymphocytes with corticosteroids or irradiation caused this effect to disappear completely suggesting that the tumor-growth-enhancing T-lymphocytes were suppressor T-cells. Furthermore thymosin (fraction 5)-treated, tumor-growth-enhancing T-lymphocytes were not able to enhance tumor growth and even significantly decreased it. In the human system we showed that Con A-stimulated lymphocytes were able to suppress the response of normal lymphocytes to PHA, PWM, and Con A, and in MLC. This effect was significantly blocked in presence of thymosin fraction 5.

Animals↗
PubMed1979

Regulation of human suppressor cell function by thymosin.

Suppressor cells were induced in vitro by Con. A using human peripheral blood lymphocytes. Suppressor cell function was evaluated by mitogen stimulation (PHA and Con. A) and in mixed lymphocyte culture (MLC), Thymosin fraction V was able to block the effector phase more significantly than the induction phase of suppressor cell expression. This effect was additive if thymosin fraction V was present both during the induction and effector phases.

Concanavalin A↗
PubMed1978

[Kinetics of decrease and apparent transformation of fluorescence spectra of benzo(a)pyrene absorbed by living cells: case of lymphocytes and macrophages].

A home made microspectrofluorimeter is used in order to follow the decrease of fluorescence intensity of Benzo(a)Pyrene after its absorption by single living cells. The kinetics look to be a first order one; fluorescent metabolite can be detected when peritoneal macrophages of Mice are used but not with human periferic lymphocytes pretreated with mitogens.

Absorption↗
PubMed1978

Quantitative comparison of K cell potential in human T and null cells.

Using a short-term quantitative assay of the antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral blood lymphocytes towards 51Cr-labeled mouse lymphoma cells, it is possible to estimate the relative involvement of various lymphocyte populations in the overall ADCC potential displayed by unfractioned lymphocytes. Lymphocytes were separated by means of an anti-F(ab')2 column combined with sedimentation of E rosette-forming cells or EA rosette-forming cells, and a discontinuous serum albumin gradient, and were characterized by several surface markers. ADCC results were expressed as the cytotoxic capacity of 10(6) cells of each population (lytic units/10(6) cells) as well as the cytotoxic potential of each population by taking into account their relative numerical sizes. Although the null cells had the highest cytotoxic capacity, highly purified T cells also disclosed a cytotoxic capacity which, because of the large numerical excess of T cells over null cells, could account for closely equivalent cytotoxic potentials of these two populations. ADCC was entirely abolished by further removal of Fc receptor-bearing-cells, in all the subsets studied. Our data underline the role that the small proportion of Fc receptor-carrying T cells might play in ADCC.

Animals↗
PubMed1977

Mouse red cell rosette formation by subpopulations of human lymphocytes.

In six separate experiments, 7-2% of purified peripheral blood lymphocytes from normal donors were shown to form rosettes with mouse erythrocytes. Normal T and B lymphocytes were separated according to their membrane properties, by E-rosette formation or fractionation on anti F (ab')2 column. The results obtained in both the separation procedures used were in good agreement: T lymphocytes were never found to form mouse red cell rosettes. On the contrary, enrichment of suspensions in B lymphocytes resulted in an increased percentage of mouse red-cell rosette-forming cells. Under the technical condition used, peroxidase-positive monocytes were not shown to form such rosettes. However, if all the mouse red cell rosette-forming cells were shown to be of B nature, not all the B lymphocytes form rosettes with mouse erythrocytes and it is not unlikely that this marker could be characteristic for a subset of Ig-bearing B lymphocytes.

Animals↗
PubMed1975

[Isolation and electrophoretic characteristics of 3 lymphocyte populations of normal human blood].

Cell electrophoresis allows separation of normal human blood lymphocytes into two main groups which are a function of their relative rates of migration, with regard to the reference speed (1 mum.sec.-1V-1.cm): the lymphocytes which have a greater mobility than this value seem to be T-lymphocytes (80,1 per cent for 42 healthy adults); on the contrary, B-lymphocytes have an inferior mobility (19,9 per cent). Two known methods are used for the selection of the lymphoid populations: spontaneous rosetting with sheep's red blood cells, which are characteristic of T lymphocytes, and adherence to nylon wool columns, which is dominant in the case of B-lymphocytes. This method confirms the fact that T-lymphocytes have a rapid migration and B-lymphocytes a slow migration. We have isolated a third population, having neither the T markers, nor the B markers. It has a very homogeneous migration, centered on the two classes 1,05 and 1,10 mum.sec.-1.V.-1.cm.

B-Lymphocytes↗