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Biomedical subjects

C Vaquero Puerta

Publications and source records attributed to C Vaquero Puerta.

At least 19 recordsLinked to original sources
PubMed2002

Laparoscopic appendectomy by ultrasonically activated scalpel in acute appendicitis: preliminary report.

BACKGROUND AND PURPOSE: Laparoscopic appendectomy (LA) is increasingly being used in treating acute appendicitis. New instruments such as the ultrasonically activated scalpel (UAS) have been introduced for most laparoscopic procedures. We evaluated the use of UAS in the performance of LA, as the potential of this instrument in this type of surgery remains to be defined. PATIENTS AND METHODS: Three patients with acute right lower abdominal pain were managed by the laparoscopic approach. Once the diagnosis of acute appendicitis was established, laparoscopic appendectomy was performed with the UAS. RESULTS: The mean operative time was 42.3 minutes (range 32-49 minutes). There were no complications related to the treatment with UAS of either the vascular pedicle or the appendicecal stump. No electrosurgical coagulation, clips, loops, or endostapler was used in any patient. CONCLUSIONS: Total LA performed with UAS is feasible. Use of the UAS may make dissection and resection of the appendix easier, helping to reduce the mean operative time.

Acute Disease↗
PubMed2002

[A study of spontaneous regeneration of defected short nerve gaps in rats].

Nerve trunk gaps can be repaired with different anastomosis methods. We investigate if spontaneous regeneration can yield results similar to artificial repair, and have compared the degree of nerve regeneration in nerve defects of extratemporal facial nerve trunk in Wistar rats. The lesions measured 4 millimeters long. Three groups were studied: 0. Normal nerve (control). 1. No treatment (therapeutic abstention). 2. Autografts. The considered parameters were: clinical outcome (degree of postoperative facial function, aspect of the defectuous region, nerve conduction latency, and number of axons that managed to penetrate the defect as well as their morphometric characteristics (nerve area). Our conclusion is that in this model placement of autografts yielded significantly better results in all the parameters studied, in spite of the scant defect length. These results likewise allow us to use this model to compare repair by means of different graft types.

Animals↗
PubMed2001

[Repair of facial nerve gaps with cryopreserved allografts in rats immunosuppressed with cyclosporin A].

UNLABELLED: The aim of this study is to assess the feasibility of peripheral nerve allografts pretreated utilizing cold storage and cyclosporin A to improve the facial nerve regeneration in Wistar rat. Three groups were designed: Normal Wistar rats. 4 millimeters nerve gaps repaired with cryopreserved nerve allografts. 4 millimeters nerve gaps repaired with cryopreserved nerve grafts and treated with cyclosporin A. At 16 weeks post-engraftment the animals were evaluated: Facial palsy grading system. Electrophysiologic latency. Axonal counting. Nervous fiber area immediately distal to the graft. The facial function of the experimental groups was similar to the control rats while the latency and morphometric parameters was poor than the normal rats. CONCLUSIONS: 1. Cryopreserved nerve allografts in facial nerve repair in rats are useful in the aquisition of a facial functional nerve recovery as much in immunosuppressed animal as not. 2. Cryopreservation of nerve allografts results a good method of storage of nerve grafts. 3. Cyclosporin A immunosuppression improved not much the grading facial palsy in this model but the electrophysiologic and morphometry is significantly better.

Anastomosis, Surgical↗
PubMed2001

[Effects of chronic alcohol drinking on rat's prostate. Experimental study].

OBJECTIVE: We have studies both the morphologic and morphometric modifications that the alcohol induces in subjected rats to chronic intake of alcohol and to evaluate the reversibility of this alterations after suppressing the ingesta of alcohol. MATERIAL AND METHODS: The animals underwent diverse outlines of chronic intake of alcohol and diverse morphometric parameters of the prostate were valued at the level of optic microscopy. RESULTS: Two morphologic prostatic patterns was observed according to the studied group of animals (experimental or control). It seems to be that the dose of alcohol was the factor that more it influenced in the morphometric variations of the cells.

Alcoholism↗
PubMed2001

[Evaluation methods of the degree of regeneration after experimental nerve repair. Evaluation model for the facial nerve in rats].

The AA. after sever the facial nerve trunk of Wistar mice (so interrupting its path owing to the loss of about 4 mm of its anatomical continuity) repair the faulty portion with several grafts, and 4 months later consider the gained results achieved in each of the following parameters: functional, morphological, biochemical and electrophysiological and, finally, compare the own outcomes with bibliographic references of literature regarding this subject.

Animals↗
PubMed2001

[Cryopreservation of neural allografts for the nerve reconstruction in experimental surgery].

The possibility of clinical employment of nervous allografts depends on the likehood of its storage and also of the need of decreasing its antigenecity. We report our methodology for cryo-preservation and storage of nervous grafts, in view of animal experimentation as well as a re-examination of other methods in order to remember grafts storages done in previous epochs.

Animals↗
PubMed2000

Acute diverticulitis and diverticular disease of the colon: a safe indication for laparoscopic surgery.

AIM: We analyzed our experience with a laparoscopic method for the treatment of acute diverticular disease. METHODS: Between January 1994 and October 1999 a group of 52 patients who fulfilled the criteria for symptomatic diverticular disease in the descending and sigmoid colon underwent laparoscopy with resection of an average of 40 cm of the bowel. Intraabdominal mechanical anastomosis completed the procedure. RESULTS: The use of ultrasonic scissors made the laparoscopic technique easier and shortened operative time. Operative morbidity was 15%. Two patients with acute diverticulitis and associated sepsis were reconverted to open surgery, and 4 patients presented postoperative rectal bleeding which ceased spontaneously. No long-term complications were found except in 1 patient who developed an incisional hernia through an entry port. Oral intake began between the second and third day. Postoperative hospitalization was 3-8 days (mean: 5.5 days) and mean operative time was 130 min (range: 70-240 min). CONCLUSIONS: Despite the steep learning curve for this type of surgery, the good morbidity and mortality rates with the laparoscopic method, especially with high-risk groups of patients (age > 65 years, high blood pressure, etc.) suggest that this surgical option can be used efficiently and safely, and that it achieves better results than with open surgery. However, we feel that the treatment of patients with acute complications of diverticular colon disease requires extensive experience with laparoscopic colorectal surgery.

Adult↗
PubMed1998

[Morphometric correlation at the experimental level of renogram with renal arterial stenosis].

RATIONALE: A reduction of the renal size seen with the nephrogram (intravenous urography) has been described in renal artery stenosis. Such reduction, however, has not been quantified and the evolution is unknown in terms of duration of stenosis evolution. METHODS: Experimental study in rats which underwent controlled stenosis of a renal artery, with evaluation, using planimetry of the area and perimeter of the nephrogram, of the consequences such stenosis had at different observation intervals (30, 90 and 180 days). The effects of the stenosis relative to the duration of the experiment is also evaluated. RESULTS: The area and perimeter of the nephrogram become reduced in kidneys with artery stenosis. Besides, this reduction increases over time. CONCLUSION: The area and perimeter of the nephrogram are a useful method to study renal artery stenosis in rats.

Analysis of Variance↗
PubMed1998

Surgical treatment of the acute cholecystitis in the laparoscopic age. A comparative study: laparoscopy against laparatomy.

OBJECTIVE: The aim of this study was to assess the complications and results of the laparoscopic opposite to open treatment of the acute cholecystitis. METHODS: A retrospective randomized study with two groups of 30 patients each one. The parameters tested were age, sex, risk factors, surgical time, hospital stay, cholecystitis type, and early or late complications. RESULTS: In the two groups there were no significant differences in age, sex, risk factors, type of cholecystitis and surgical time. The average of hospital stay was significantly longer for open cholecystectomy (9.5) than for laparoscopic technique (2.30) (p < 0.001). The complication rate was higher (7.30%) in open cholecystectomy. CONCLUSIONS: The laparoscopic cholecystectomy should be the standard procedure for the treatment of the acute cholecystitis.

Acute Disease↗
PubMed1997

Repair of experimental mandibular defects in rats with autogenous, demineralised, frozen and fresh bone.

This prospective experimental study aimed to assess the regenerative capability of demineralised bone autografts resected and replaced orthotopically, compared with traditional fresh and deep frozen mandibular autografts in rats. In 60 adult Wistar rats, a bone defect 4 x 4 mm was created at the left ascending mandibular ramus and the removed bone was used as a fresh (n = 20), deep frozen (n = 20), or demineralised (n = 20) graft which was implanted orthotopically 2 weeks later. Ten rats in each group were killed at 2 and 6 weeks later. Outcome was measured by cellular proliferation on histological examination. The number of mesenchymal cells was significantly greater (P < 0.05) at both 2 and 6 weeks in the demineralised grafts than in the other two groups. There were no differences between the 2- and 6-week examinations of deep frozen bone, nor between the medullary and peripheral aspects. It was concluded that demineralised bony autografts cause greater osteoinduction both in the short (2 weeks) and the medium (6 weeks) term.

Animals↗
PubMed1997

Octreotide: effects in experimental severe acute pancreatitis. Analysis of survival, biochemical findings and histomorphometry.

OBJECTIVE: The effect of octreotide in acute experimental pancreatitis was examined. EXPERIMENTAL DESIGN: Acute pancreatitis was induced in 70 male Wistar rats by retrograde injection of 5% sodium taurocholate into the pancreatic duct. Octreotide (50 micrograms = 0.5 ml) or isotonic saline was administered subcutaneously every six hours for 24 hours, beginning at the time of induction of pancreatitis (groups 1 and 2) or 12 hours later (groups 3 and 4). Six hours after the last dose of octreotide or saline, the rats were sacrificed. Ten additional rats were used for basal biochemical determination. For the analysis of survival, another 10 rats were included. The survival rate, serum concentrations of amylase, lipase and lactate dehydrogenase (LDH) and morphometric studies (total area of the pancreas and percentage necrosis) were examined. Statistical analysis involved Student's t test, ANOVA and the Mantel-Haenszel test. RESULTS: No significant differences existed among the groups with respect to survival, morphometric analysis or biochemical determinations, except for a minor increase in LDH levels in the group treated with octreotide at the time of induction. CONCLUSIONS: Octreotide administration has almost no impact on mortality in experimental severe acute pancreatitis. Biochemical and morphometric changes are minimal. Therefore, according to the present study, the efficacy of octreotide administration is very low.

Acute Disease↗
PubMed1997

[Testicular changes produced by alcohol].

Acting as a direct toxic through different mechanisms, alcohol causes many hormonal changes which induce sexual function impairment and fertility problems. We have used 36 white Wistar Lewis rats, divided in three large groups: 10% alcoholic, 25% alcoholic and control group for 55 and 95 days. The parameters studied were the rat's ponderal evolution, testicular volume and weight, testes and body weights ratio, as well as testes density and seminiferous tubule area. We found a decreased ponderal gain in animals receiving high doses of alcohol. Decreased testicular volume and increased density in long-term alcohol regime. At high dose and medium-term increased testicular weights were seen as compared to total weight of the rat. The area of the seminiferous tubule is reduced in all alcoholic groups.

Animals↗
PubMed1995

Effect of particulate porous hydroxyapatite on osteoinduction of demineralized bone autografts in experimental reconstruction of the rat mandible.

In an experimental model in the rat, a nonhealing bone defect was created in the left ascending mandibular ramus to test the effect of particulate porous hydroxyapatite (HA) on osteoinduction of demineralized bone autografts. The bone fragment removed was demineralized in HCl and used as an autograft for mandibular reconstruction. Granules of HA were added to the lingual and vestibular surfaces of the graft. The effect of this material was evaluated by determining the number of mesenchymal cells induced in the biomaterial and the central and peripheral zones of the bone graft, at 2-and 6-week intervals. The results show that the sites containing HA showed inhibition of osteoinduction by the bone matrix. In all groups, a proliferative gradient from the peripheral zone toward the center of the bone was observed. Similarly, the HA experienced a greater cellular increase in the regions in contact with the demineralized bone matrix.

Animals↗
PubMed1992

[Perianal ulcers of tubercular origin. A report of 3 new cases].

The rate of patients with extrapulmonary tuberculosis has increased in the last years (about 5% of all cases), and displaying a wide spectrum of clinical manifestations. Among them, the anal localization is a rare occurrence (0.7%) according to available published data. During the last two years, we have diagnosed and treated three patients with perianal tuberculous lesions manifested by ulcers. All three were males, associated with active pulmonary tuberculosis, and one with intestinal involvement which required surgical treatment. Based on this experience, we have reviewed the different etiopathogenic, clinical, diagnostic and therapeutic manifestations of this picture, emphasizing the importance of suspicion, and its diagnostic easiness if the different infectious and inflammatory diseases at this level are taken into account.

Abscess↗