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Biomedical subjects

C Wainwright

Publications and source records attributed to C Wainwright.

10 recordsLinked to original sources
PubMed2004

Total body water in children with cystic fibrosis using bioelectrical impedance.

BACKGROUND: The aim of this study was to assess the ability of commercially available single frequency bioelectrical impedance analysis (BIA) to predict total body water and hence body composition in a cohort of children with cystic fibrosis (CF). METHODS: In 56 (31 girls, 25 boys) children aged 4 to 18 years, total body water was predicted using BIA and measured using a deuterium oxide dilution technique. Each child provided a urine sample before consuming 0.5 g/kg body weight 10% deuterium oxide. A further urine sample was collected between 4 and 6 h later. RESULTS: There was a mean bias between predicted and measured total body water of 1.1 L. The bias was consistent across the range of total body water measured in this study. The relationship between height2/impedance and measured total body water was TBW=0.35+0.74*height2/impedance. This equation is similar to other equations found in healthy children. CONCLUSION: We have shown that a commercially available bioelectrical device underestimates measured total body water. We have generated a prediction equation for the population studied, which we believe might enhance the accuracy of the method in children with cystic fibrosis.

Adolescent↗
PubMed2002

Series of laryngomalacia, tracheomalacia, and bronchomalacia disorders and their associations with other conditions in children.

Laryngomalacia, bronchomalacia, and tracheomalacia are commonly seen in pediatric respiratory medicine, yet their patterns and associations with other conditions are not well-understood. We prospectively video-recorded bronchoscopic data and clinical information from referred patients over a 10-year period and defined aspects of interrelationships and associations. Two hundred and ninety-nine cases of malacia disorders (34%) were observed in 885 bronchoscopic procedures. Cough, wheeze, stridor, and radiological changes were the most common symptoms and signs. The lesions were most often found in males (2:1) and on the left side (1.6:1). Concomitant malacia lesions ranged from 24% for laryngotracheobronchomalacia to 47% for tracheobronchomalacia. The lesions were found in association with other disorders such as congenital heart disorders (13.7%), tracheo-esophageal fistula (9.6%), and various syndromes (8%). Even though the understanding of these disorders is in its infancy, pediatricians should maintain a level of awareness for malacia lesions and consider the possibility of multiple lesions being present, even when one symptom predominates or occurs alone.

Adolescent↗
PubMed2001

UK survey of attitudes to local anaesthesia for vitreoretinal surgery.

BACKGROUND: The use of local anaesthesia (LA) for vitreoretinal (VR) surgery is growing although surgeons in the UK traditionally use general anaesthesia. PURPOSE: To assess the use and attitudes towards local anaesthesia for VR surgery among the members of the British and Eire Association of Vitreo-retinal Surgeons (BEAVRS). METHODS: A questionnaire was sent to members of BEAVRS. Use of LA was assessed for the following procedures: macular hole; diabetic vitrectomy; vitrectomy; cryopexy and gas; buckling and re-buckling procedures. Attitudes of surgeons generally using LA for primary retinal detachment surgery (> 50% of cases) were compared with those generally using general anaesthesia (GA) (> 50% of cases). RESULTS: 33.6% of surgeons preferred LA for macular hole; 26.2% for diabetic vitrectomy; 23.3% for vitrectomy, cryopexy and gas; 21.4% for buckling procedures; and 9.35% for re-buckling procedures. When surgeons routinely using LA were compared with those regularly using GA they considered LA less stressful for patients and surgeon, patients tolerant to longer operations, and buckling or redo surgery no more difficult under LA. However, both groups considered surgery on young patients and teaching relatively more difficult under LA. CONCLUSION: The use of LA may convey benefits for VR surgery and faster rehabilitation for patients. However, we identified wide variations in the use of and attitudes to LA for VR surgery in British surgeons.

Adult↗
PubMed1997

Asthma in children.

Explore the source record for details and available documents.

Administration, Inhalation↗
PubMed1996

Resting energy expenditure, pulmonary inflammation, and genotype in the early course of cystic fibrosis.

To further investigate the early course of cystic fibrosis (CF), specifically to examine factors contributing to energy imbalance, we examined resting energy expenditure (REE) (by indirect calorimetry) per unit body weight and metabolically active body cell mass (by total body potassium), in relation to CF genotype (by genomic DNA analysis), CF pancreatic phenotype, and markers of pulmonary inflammation (from bronchoalveolar lavage fluid). Eighteen subjects with presymptomatic CF who were less than 2 years of age (n = 11, delta F508/ delta F508 genotype; n = 15, pancreatic insufficiency phenotype), identified by newborn screening, were compared with age-, sex-, and length-matched control subjects (n = 13). Those with the delta F508/ delta F508 genotype had significantly higher mean REE expressed per unit body weight (125%) and body cell mass (115%; p < 0.03). Those with other genotypes (n = 7) did not, as a group, have significantly different mean REEs, but individuals with known "severe" genotypes had REEs in the high range and those with a pancreatic-sufficient phenotype had significantly lower REE than those with a pancreatic-insufficient phenotype (p < 0.05), and REEs were in the normal range. Examination of bronchoalveolar lavage fluid revealed positive culture results (7/10) but variable colony counts, neutrophil percentages, and concentrations of interleukin-8 and interleukin-1 beta equally in both CF genotype groups. These markers of pulmonary inflammation were not correlated, individually or collectively, with REE or genotype. We conclude that genotypic variations in energy balance are detectable early in CF unrelated to lung inflammation. Subclinical defects in body composition and pulmonary integrity occur early in CF and, in combination with increased cellular metabolic activity, have important clinical implications with respect to early diagnosis and management.

Body Composition↗
PubMed1992

Accumulation of a Brazil nut albumin in seeds of transgenic canola results in enhanced levels of seed protein methionine.

We have increased the methionine content of the seed proteins of a commercial winter variety of canola by expressing a chimeric gene encoding a methionine-rich seed protein from Brazil nut in the seeds of transgenic plants. Transgenic canola seeds accumulate the heterologous methionine-rich protein at levels which range from 1.7% to 4.0% of the total seed protein and contain up to 33% more methionine. The precursor of the methionine-rich protein is processed correctly in the seeds, resulting in the appearance of the mature protein in the 2S protein fraction. The 2S methionine-rich protein accumulates in the transgenic seeds at the same time in development as the canola 11S seed proteins and disappears rapidly upon germination of the seed. The increase in methionine in the canola seed proteins should increase the value of canola meal which is used in animal feed formulations.

Gene Expression↗
PubMed1991

Postoperative myocardial damage in patients with coronary artery disease undergoing major non cardiac surgery.

A prospective study was carried out in a group of 50 patients with coronary artery disease, presenting for major non-cardiac surgery, to investigate the timing and incidence of further perioperative myocardial damage. A standardised anaesthetic was used. A standard 12-lead ECG was taken immediately before surgery and at 24, 48, and 72 hr after the start of anaesthesia. Blood samples were taken immediately preoperatively and at 6, 24, 48, and 72 hr after anaesthesia for total CK and CK-MB assay. Thirty-three patients (66%) showed ECG evidence suggestive of further infarction, and of these, two (4%) died in the immediate perioperative period. The first ECG change occurred in 27/31 (87%) by 24 hr, in 3/31 (10%) by 48 hr, and 1/31 (3%) by 72 hr. Twenty-nine patients (58%) including the two deaths showed CK-MB enzyme changes. The first elevation in CK-MB was nil at 6 hr and 72 hr, with 23/27 (85%) at 24 hr, and 4/27 (15%) at 48 hr. In 22/50 (44%) ECG and enzymes were correlative. Goldman and Cooperman risk indices were calculated for each patient. The Cooperman risk index was superior to the Goldman scale in the correlation of observed with predicted myocardial morbidity. Patients with ECG changes only before surgery were just as liable to further myocardial damage as those patients with ECG changes and a documented history of a previous infarct and/or symptoms. Myocardial damage is maximal in the first 24 hr after surgery, and may not be adequately predicted by current risk indices.

Adult↗
PubMed1991

Early postoperative myocardial morbidity in patients with coronary artery disease undergoing major non-cardiac surgery: correlation with perioperative ischaemia.

As a part of a study assessing early postoperative myocardial morbidity in 50 patients with active coronary artery disease undergoing major non-cardiac surgery, the ECG was monitored continuously for 24 hr after the onset of anaesthesia, using a frequency modulated (FM) Holter monitor. Concurrent automated blood pressure and pulse were measured non-invasively at three-minute intervals during anaesthesia and subsequently at five-minute intervals. Thirty patients were monitored with two-site ECG recordings, from modified V1 and V5 (Group A). Twenty patients had seventeen-site ECG monitoring, multiplexing a four by four array of precordial electrodes onto one channel of the frequency modulated recorder (Group B). Tapes were analyzed for noise, supraventricular and ventricular dysrythmias, runs of tachy- and bradycardia, and ST segment changes. These data were correlated with serial standard 12-lead ECGs and CK-MB assay in the 72 hr after surgery. Seven tapes from Group A could not be analyzed. Change (greater than 1 mm) on ST monitoring from both Groups A (14/23), B (14/20), correlated with serial 12-lead ECG and/or CK-MB changes. The majority of first ST change 19/28 (70%) occurred after anaesthesia. In 14/28 (50%) ST change occurred during episodes of tachycardia and elevated blood pressure (greater than 20% above baseline). Nine patients (9/23) in Group A had no ST change; however, six had serial 12-lead ECG and/or CK-MB changes. Six patients (6/20) in Group B had no ST changes, and none of these patients had any change of serial 12-lead ECGs or CK-MB assay. No patient complained of chest pain during the Holter monitoring period. Continual monitoring of heart rate and blood pressure and accurate ST monitoring are essential to detect and treat perioperative myocardial ischemia. A multiple-lead precordial system is substantially more sensitive than traditional two-lead ECG holter monitoring in detecting myocardial ischaemia.

Arrhythmias, Cardiac↗