Biomedical subjects

C Wehrli

Publications and source records attributed to C Wehrli.

6 recordsLinked to original sources

PubMed2001

[Low molecular weight heparin (dalteparin) in treatment of patients with thromboembolism incidents].

UNLABELLED: Low molecular weight heparine (LMWH) is an established treatment for deep venous thrombosis during the initial phase of the illness as well as for prophylaxis of thrombosis. There are only few studies concerning the use of LMWH in the initial treatment of lung embolism. 138 Patients with either deep venous thrombosis at least reaching the vena poplitea and/or lung embolism have been randomised into two groups. One group was treated with unfragmented heparin. The second group received a single dose of 200 I.E./kg body weight/d LMWH (Dalteparin). The results were compared with regard to the recurrence of thrombosis and embolism, bleeding, heparin-induced thrombopenia and mortality. RESULTS: A total of 138 patients were randomised. 13 patients were excluded later. The two groups were comparable regarding age, sex, weight and diagnosis. The average treatment lasted 8.3 (5-26) days in the LMWH-group and 7.4 (4-14) days in the heparine-group. The average hospital stay was 10.4 days in the LMWH-group, and 11.0 days in the heparine-group. The complication rate was 5 (3.1%) (5 events: 3 hemorrhages, 1 reembolism, 1 death) in the LMWH-group and 4 (2.5%) (4 events: 3 bleedings, 1 sepsis of the venous catheter) in the heparine-group. We found no significant outcome difference between the two treatment groups. CONCLUSION: Weight-adapted (200 IE/kg/day) subcutaneous application of Dalteparin once daily in the treatment of patients with deep venous thrombosis or lung embolism is as safe as intravenous treatment with unfragmented heparine.

Aged↗

PubMed2000

[Differential diagnostic considerations in CK-MB level increase].

We present two patients who were hospitalized due to thrombo-embolic disease. Both patients had an increase in total creatine kinase activity with the creatine kinase MB fraction value exceeding the total creatine kinase activity. We determined that the high values for creatine kinase MB fraction in the immunoinhibition assay were due to the existence of macro creatine kinase type I in one patient and a highly elevated creatinine kinase BB fraction in the other patient. The patient with macro CK type I had ulcerative colitis and the other patient with elevated CK BB fraction was diagnosed with prostatic carcinoma.

Aged↗

PubMed1996

Comparison of genetic groups determined by molecular and immunological analyses of Giardia isolated from animals and humans in Switzerland and Australia.

Nine axenic isolates of Giardia originating from four different host species in Switzerland were subjected to genetic analysis using the polymerase chain reaction (PCR) to amplify segments of genes encoding different trophozoite variant surface proteins (VSPs). Three genotypes were identified on the basis of product yield, size, and restriction-fragment-length polymorphisms (RFLPs). Five G. duodenalis isolates (O1, B1, B2, B3-1A1 and C1--from a sheep, three calves and a dog, respectively) were classified as belonging to genetic group I of Andrews et al. (1989). DNA amplified from the VSP genes tsp11, tsa417 and vsp1267 of these isolates was indistinguishable in size and restriction characteristics from that amplified from group-I Giardia isolated from humans in Australia. One human-derived Swiss isolate (H2-17A1), typed as belonging to genetic group II, yielded a vsp1267-specific PCR product that was indistinguishable by size or restriction sites from the equivalent 1.6-kb product amplified from human-derived Australian group-II organisms. This isolate also yielded 1.8-kb tsp11 and 0.52-kb tsa417/tsp11-like PCR products possessing RFLPs typical of group-II organisms. Three isolates (O2-4A1, O3 and H3-15K2--originating from two sheep and a human, respectively) represent a novel genotype that is closely related to genetic groups I and II. These three isolates exhibited identical RFLPs in their tsp11 PCR products and failed to yield a vsp1267 PCR product. An antiserum specific for the 90-kDa VSP of the sheep-derived clone O2-4A1 reacted strongly by immunofluorescence and on Western blots with surface proteins from the O2, O3 and H3 isolates only--consistent with the genetic classification determined above. The data provide no evidence for the occurrence of host-specific genotypes.

Animals↗

PubMed1996

Cloning and characterization of the gene encoding pyruvate phosphate dikinase from Giardia duodenalis.

This paper reports the cloning and molecular characterization of the gene encoding pyruvate phosphate dikinase (PPDK) from Giardia. The ORF is 2652 nucleotide residues in length and not interrupted by introns. The gene appears to exist as a single copy in the genome and predicts a 97629 Da protein containing 884 amino acid residues. Comparison of the deduced Giardia PPDK sequence with those of homologous enzymes from other organisms revealed high sequence similarities and the presence of various conserved domains known to be essential for substrate binding and catalysis. Analysis of the ppdk gene and 19 other protein-coding genes from the protist revealed no typical TATA boxes, positioned at around -30, but the presence of two novel consensus sequence motifs in the 5' flanking regions. One is an AT-rich element immediately preceding the translation initiation codon and the other a 14-bp box centered at -30. These shared consensus sequence patterns present in the 5' flanking region of Giardia genes are suggested to play a role in the control of transcription initiation.

Animals↗

PubMed1994

The mannose transporter of Escherichia coli K12: oligomeric structure, and function of two conserved cysteines.

The mannose transporter of E. coli is a member of the phosphotransferase system. It consists of two membrane spanning subunits, IICMan (27.64 kDa) and IIDMan (31.02 kDa) and a peripheral subunit IIABMan (35.02 kDa). It acts by a mechanism that couples vectorial translocation to phosphorylation of the substrate. The subunit ratio determined from densitometric scans of polyacrylamide gels is close to IIABMan2 IICMan1 IIDMan2. A molecular mass of 100 +/- 20 kDa was calculated from electronmicrographs of freeze fractured proteoliposomes containing particles of the IICMan/IIDMan subcomplex with a mean diameter of 6.3 +/- 1.1 nm. This is most compatible with IICMan:IIDMan subunit compositions of 1:2 (89.7 kDa). Fusion proteins between IICMan and IIDMan were generated, with the subunits connected either by a two-residue linker or a 20 residue Ala Pro rich hinge. The fusion proteins had 5%-15% of control phosphotransferase activity. The one with the Ala Pro rich linker could be cleaved with trypsin resulting in a 7 fold increase of activity while the fusion with the two residue linker was resistant to limited trypsinolysis. Taking into account the inside-out orientation of the membrane vesicles the C-terminus of IICMan and the N-terminus of IIDMan are both predicted to be on the cytoplasmic side of the membrane. Two cysteines in IICMan and IIDMan which are conserved in the homologous subunits of the fructose transporter of Bacillus subtilis and of sorbose transporter of Klebsiella pneumoniae are not necessary for phosphotransferase function.

Amino Acid Sequence↗

PubMed1991

Impaired affinity for phenylalanine in Escherichia coli phenylalanyl-tRNA synthetase mutant caused by Gly-to-Asp exchange in motif 2 of class II tRNA synthetases.

Phenylalanyl-tRNA synthetase (PheRS; alpha 2 beta 2 subunit structure) is a member of class II of tRNA synthetases. We report here the genetic analysis of an Escherichia coli mutant strain which is auxotrophic for phenylalanine because it has a PheRS with a decreased affinity for phenylalanine. The mutant pheS gene encoding the PheRS alpha subunit was cloned and sequenced, and the deviation from the wild-type gene was found to result in a Gly191-to-Asp191 exchange. This alteration is located within motif 2, one of 3 conserved sequence motifs characteristic for class II aminoacyl-tRNA synthetases. Motif 2 may thus participate in the formation of the phenylalanine binding site in PheRS.

Amino Acid Sequence↗